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A Comparison of Various Growth Parameters of Cell Suspension Cultures to Determine Phytotoxicity of Xenobiotics

Published online by Cambridge University Press:  12 June 2017

David G. Davis ,
Rosa L. Stolzenberg  and
Joan A. Dusky
David G. Davis
Affiliation:
U.S. Dep. Agric., Agric. Res. Serv. Metabolism and Radiation Res. Lab., Fargo, ND 58105
Rosa L. Stolzenberg
Affiliation:
U.S. Dep. Agric., Agric. Res. Serv. Metabolism and Radiation Res. Lab., Fargo, ND 58105
Joan A. Dusky
Affiliation:
Bot. Dep., North Dakota State Univ., Fargo, ND 58105
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Abstract

An assessment was made of various parameters to measure growth of soybean [Glycine max (L.) Merr. ‘Wilkin’] and einkorn (Triticum monococcum L.) cell suspension cultures to establish convenient methods of screening the effects of chemicals. Methods assessed were settled cell volumes, packed cell volumes, absorbance at 525 nm of sonicated aliquots, dry weights (of aliquots or entire flask contents), and electrical conductivity and pH of the culture medium. Settled cell volumes, conductivity, and dry-weight changes were the most useful of the methods tested for determining the phytotoxicity of a nonionic linear alcohol ethylene oxide detergent (an adduct of 1-dodecanol containing eight ethylene oxide units) and the methyl ester of diclofop {2-[4-(2,4-dichlorophenoxy)phenoxy] propanoic acid}. Because 3 to 4 weeks were required to assess whether the cultures could grow out of the initial inhibition by the detergent or herbicide, none of the methods was rapid. Advantages and disadvantages of the various methods and their relative values for screening compounds are described.

Keywords

SoybeaneinkornphytotoxicityGlycine maxTriticum monococcumdetergentdiclofop-methyl
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 32 , Issue 2 , March 1984 , pp. 235 - 242
Copyright
Copyright © 1984 by the Weed Science Society of America 

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References

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