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Confirmation of Flixweed (Descurainia sophia) Resistance to Tribenuron-Methyl Using Three Different Assay Methods

Published online by Cambridge University Press:  20 January 2017

Xian Xu ,
Gui Qi Wang ,
Si Long Chen ,
Cui Qin Fan  and
Bing Hua Li
Xian Xu
Affiliation:
Institute of Food and Oil, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050031, China
Gui Qi Wang*
Affiliation:
Institute of Food and Oil, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050031, China
Si Long Chen
Affiliation:
Institute of Food and Oil, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050031, China
Cui Qin Fan
Affiliation:
Institute of Food and Oil, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050031, China
Bing Hua Li
Affiliation:
Institute of Food and Oil, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050031, China
*
Corresponding author's E-mail: guiqiwang@gmail.com
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Abstract

Research was conducted to establish a method to investigate the resistance level of flixweed to tribenuron-methyl and the evolved biochemical resistance mechanism. Four resistant biotypes were collected from wheat fields in Mazhuangcun, Jiacun, Dishangcun, and Bafangcun in the Hebei province of China where tribenuron-methyl had been continuously used for more than 10 yr. Two susceptible biotypes were collected from wheat fields where tribenuron-methyl was never applied. Different biotypes were assessed by petri-dish bioassay, whole-plant bioassay, and acetolactate synthase (ALS) assay. Comparisons of data indicated a similarity between methods and that experiments demonstrated that petri-dish bioassay was a feasible method to identify flixweed resistant to tribenuron-methyl. Data indicated differences among the flixweed biotypes when assessed by the petri-dish bioassay, whole-plant bioassay, or ALS enzyme assay, and a close association was obtained for the three bioassay methods. ALS resistance varied by biotypes with Mazhuangcun > Jiacun > Dishangcun > Bafangcun. Target-site enzyme assay data indicated that the resistant biotype's enhanced ALS activity was the biochemical mechanism that induced flixweed's evolved resistance to tribenuron-methyl. The concentrations of tribenuron-methyl causing 50% inhibition of ALS activity of the four resistant biotypes were 1,359, 513, 184, and 164 nM; in the susceptible biotypes these concentrations were 64 and 65 nM. Resistance indexes were 21, 8, 3, and 3 for Mazhuangcun, Jiacun, Dishangcun, and Bafangcun biotypes, respectively.

Keywords

Tribenuron-methylflixweed, Descurainia sophia (L.) Webb ex Prantl DESSOwheat, Triticum aestivum L.Resistanceacetolactate synthase
Type
Weed Biology and Ecology
Information
Weed Science , Volume 58 , Issue 1 , March 2010 , pp. 56 - 60
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

Beckie, H. J., Friesen, L. F., Nawolsky, K. M., and Morrison, I. N. 1990. A rapid bioassay to detect trifluralin-resistant green foxtail (Setaria viridis). Weed Technol. 4:505–508.CrossRefGoogle Scholar
Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248–254.CrossRefGoogle ScholarPubMed
Corbett, C. A. L. and Tardif, F. J. 2006. Detection of resistance to acetolactate synthase inhibitors in weeds with emphasis on DNA-based techniques: a review. Pest Manag. Sci. 62:584–597.CrossRefGoogle ScholarPubMed
Cotterman, J. C. and Saari, L. L. 1992. Rapid metabolic inactivation is the basis for cross-resistance to chlorosulfuron in diclofop-methyl resistant rigid ryegrass (Lolium rigidum) biotype SR4/84. Pest. Biochem. Physiol. 43:182–192.CrossRefGoogle Scholar
Cui, H. L., Zhang, C. Z., Zhang, H. J., Liu, X., Liu, Y., Wang, G. Q., Huang, H. J., and Wei, S. H. 2008. Confirmation of flixweed (Descurainia sophia) resistance to tribenuron in China. Weed Sci. 56:775–779.CrossRefGoogle Scholar
Defelice, M. D., Guardiola, J., Esposito, B., and Iaccarino, M. 1974. Structural genes for a newly recognized acetolactate synthase in Escherichia coli K-12. J. Bacteriol. 120:1068–1077.Google Scholar
Heap, I. M. 2009. International Survey of Herbicide Resistant Weeds. http://www.weedscience.com. Accessed: January 5, 2009.Google Scholar
Huang, S. X. 2004. Studies on biology and resistance of Alopecurus aequalis Sobol. to acetyl-coenzyme A carboxylase inhibitors. . Nanjing, China Nanjing Agricultural University.Google Scholar
InKuk, Y., Burgos, N. R., and Talbert, R. E. 2000. Cross- and multiple resistance of diclofop-resistant Lolium spp. Weed Sci. 48:412–419.Google Scholar
InKuk, Y., Jung, H., Kwon, O. D., Lee, D. J., Burgos, N. R., and Guh, J. O. 2003. Rapid diagnosis of resistance to sulfonylurea herbicides in monochoria (Monochoria vaginalis). Weed Sci. 51:305–311.Google Scholar
Letouze, A. and Gasquez, J. 1999. A rapid reliable test for screening aryloxyphenoxypropionic acid resistance within Alopecurus myosuroides and Lolium spp. populations. Weed Res. 39:37–48.CrossRefGoogle Scholar
Li, X. J., Wang, G. Q., Fan, C. Q., and Duan, M. S. 2004. Weeds and their chemical control in winter wheat in Hebei Province. Hebei J. Agric. Sci. 8:14–17 [In Chinese].Google Scholar
Maxwell, B. D. and Mortimer, A. M. 1994. Selection for herbicide resistance. Pages 1–26. in Powles, S. B. and Holtum, J. A. M. Herbicide Resistance in Plants: Biology and Biochemistry. Boca Raton, FL Lewis.Google Scholar
Moss, S. R. 1995. Techniques for determining herbicide resistance. Brighton Crop Protection Conference—Weeds. 2:547–556.Google Scholar
Murray, B. G., Friesen, L. F., Beaulieu, K. J., and Morrison, I. N. 1996. A seed bioassay to identify acetyl-CoA carboxylase inhibitor resistant wild oat (Avena fatua) populations. Weed Technol. 10:85–89.CrossRefGoogle Scholar
Park, K. W. and Mallory-Smith, C. A. 2004. Physiological and molecular basis for ALS inhibitor resistance in Bromus tectorum biotypes. Weed Res. 44:71–77.CrossRefGoogle Scholar
Ray, T. B. 1984. Site of action of chlorsulfuron inhibition of valine and isoleucine biosynthesis in plants. Plant Physiol. 75:827–831.CrossRefGoogle ScholarPubMed
Saari, L. L., Cotterman, J. C., and Primiani, M. M. 1990. Mechanism of sulfonylurea herbicide resistance in the broad leaf weed, Kochia scoparia . Plant Physiol. 93:55–61.CrossRefGoogle Scholar
Saari, L. L., Cotterman, J. C., Smith, W. F., and Primiani, M. M. 1992. Sulfonylurea herbicide resistance in common chickweed, perennial ryegrass, and Russian thistle. Pest. Biochem. Physiol. 42:110–118.CrossRefGoogle Scholar
Seefeldt, S. S., Jensen, J. E., and Fuerst, E. P. 1995. Log-logistic analysis of herbicide dose-response relationships. Weed Technol. 9:218–225.CrossRefGoogle Scholar
Smeda, R. J., Barrentine, W. L., Snipes, C. E., and Rippee, J. H. 1995. Identification of johnsongrass resistance to graminicides and alternative control methods. Pages 262–263. in. Proceedings of the International Symposium on Weed and Crop Resistance to Herbicides. Cordoba.Google Scholar
Sun, B. Y. 1996. Studies on growth and decline of Alopecurus weed populations in wheats and identification of chlortoluron resistance. . Nanjing, China Nanjing Agricultural University.Google Scholar
Tal, A., Syka, E. K., and Rubin, B. 2000. Seed-bioassay to detect grass weeds resistant to acetylcoenzyme A carboxylase inhibiting herbicides. Crop Protect. 19:467–472.CrossRefGoogle Scholar
Tranel, P. J. and Wright, T. R. 2002. Resistance of weeds to ALS-inhibiting herbicides: what have we learned? Weed Sci. 50:700–712.CrossRefGoogle Scholar
Trucco, F., Hager, A. G., and Tranel, P. J. 2006. Acetolactate synthase mutation conferring imidazolinone-specific herbicide resistance in Amaranthus hybridus . Plant Physiol. 163:475–479.CrossRefGoogle ScholarPubMed
Xu, X., Wang, G. Q., Zhang, H. J., Fan, Q. C., Li, B. H., Su, L. J., and Wang, J. P. 2008. Preliminary study on the resistance of Descurainia sophia to tribenuron-methyl in Hebei Province. Acta Agri. Boreali-occidentalis Sinica. 17:270–273. [In Chinese].Google Scholar
Yang, C. H., Dong, L. Y., Li, J., and Moss, S. R. 2007. Identification of Japanese foxtail (Alopecurus japonicus) resistant to haloxyfop using three different assay techniques. Weed Sci. 55:537–540.CrossRefGoogle Scholar
Zhou, S. D. and Luo, P. 1998. The effects of various chemicals on breaking dormancies of Descrainia Sophia seeds. J. Sichuan Teachers College. 19:300–303. [In Chinese].Google Scholar