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ρ-Cymene Inhibits Growth and Induces Oxidative Stress in Rice Seedling Plants

Published online by Cambridge University Press:  20 January 2017

Fengjuan Zhang ,
Fengxin Chen ,
Wanxue Liu ,
Jianying Guo  and
Fanghao Wan
Fengjuan Zhang
Affiliation:
College of life Science, Hebei University, Baoding, Hebei, 071002, China State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection (South Campus), Chinese Academy of Agricultural Sciences, Beijing, 100081, China
Fengxin Chen
Affiliation:
College of life Science, Hebei University, Baoding, Hebei, 071002, China
Wanxue Liu
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection (South Campus), Chinese Academy of Agricultural Sciences, Beijing, 100081, China
Jianying Guo
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection (South Campus), Chinese Academy of Agricultural Sciences, Beijing, 100081, China
Fanghao Wan*
Affiliation:
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection (South Campus), Chinese Academy of Agricultural Sciences, Beijing, 100081, China
*
Corresponding author's E-mail: wanfh@caas.net.cn
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Abstract

ρ-Cymene was one of the major components of volatiles released by croftonweed. The allelopthy of ρ-cymene on the growth of upland rice seedlings was performed. Hydrogen peroxide generation, malondialdehyde (MDA) content, proline content, total ascorbate (ascorbate/dehydroascorbate), reduced/oxidized glutathione, and the levels of induction of antioxidant enzyme were studied in the seedlings of upland rice. ρ-Cymene inhibited the growth of upland rice seedlings. Exposure of upland rice seedlings to ρ-cymene increased levels of H2O2, MDA, and proline, indicating lipid peroxidation and induction of oxidative stress. Activities of the antioxidant enzymes superoxide dismutase, catalase, peroxidase, guaiacol peroxidase, ascorbate peroxidase, and glutathione reductase were significantly elevated during the treatment period (7–15 d) compared with enzymes in the upland rice seedlings unexposed to ρ-cymene, thereby indicating the enhanced generation of reactive oxygen species (ROS) upon ρ-cymene exposure. These results suggest that activation of the antioxidant system by ρ-cymene led to the formation of ROS that resulted in cellular damage and decreased growth of upland rice seedlings.

Keywords

Croftonweed, Ageratina adenophora (Sprenge) King & H.E. Robins. (synonym: Eupatorium adenophora Sprenge)rice, Oryza sativa Lρ-Cymenereactive oxygen speciesantioxidant enzymes
Type
Weed Biology and Ecology
Information
Weed Science , Volume 60 , Issue 4 , December 2012 , pp. 564 - 570
Copyright
Copyright © Weed Science Society of America 

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