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Effect of Chlorimuron and Quizalofop on Fatty Acid Biosynthesis

Published online by Cambridge University Press:  12 June 2017

Leslie A. Bjelk  and
Thomas J. Monaco
Leslie A. Bjelk
Affiliation:
Dep. Hortic. Sci., North Carolina State Univ., Raleigh, NC 27695-7609
Thomas J. Monaco
Affiliation:
Dep. Hortic. Sci., North Carolina State Univ., Raleigh, NC 27695-7609
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Abstract

Chlorimuron antagonized the activity of quizalofop on broadleaf signalgrass when applied in greenhouse studies as a postemergence tank mix. In vitro leaf disc assays utilizing 14C-acetate or 14C-pyruvate as substrates were conducted to ascertain the effect of clorimuron and quizalofop on fatty acid biosynthesis and to determine if antagonism between the two herbicides occurs at the biochemical sites of action. Incorporation of 14C-acetate in control treatments was linear with time to 120 min. Acetate incorporation in the presence of quizalofop (1.1 μM) was also linear but was inhibited 30 min after initialization of the reaction. The concentration of quizalofop that inhibited 14C-acetate incorporation 50% (I50) was 0.54 μM. Chlorimuron, up to 155 μM, had no effect on 14C-acetate incorporation. A mixture of quizalofop (1.1 μM) and chlorimuron (4.8 μM) inhibited 14C-acetate incorporation similar to that of quizalofop alone at 1.1 μM. Quizalofop I50 for incorporation of 14C-pyruvate was 1.1 μM, and clorimuron at 4.8 μM decreased incorporation 15%. Excess unlabeled pyruvate (5 μM) had no effect on either 14C-acetate or 14C-pyruvate incorporation in the presence of both herbicides. It is believed that antagonism of quizalofop by clorimuron is not due to an excess pool of pyruvate resulting from inhibition of acetolactate synthase by chlorimuron.

Keywords

Chlorimuron, 2-[[[[(4-chloro-6-methoxy-2-pyrimidinyl)amino]carbonyl]amino]sulfonyl] benzoic acidquizalofop, (±)-2-[4[(6-chloro-2-quinoxalinyl)oxy]phenoxy] propanoic acidbroadleaf signalgrass (Bracharia platyphylla (Griseb.) Nash # BRAPP)Antagonismacetyl coenzyme A carboxylaseacetolactate synthasepyruvateacetateBracharia platyphyllaBRAPP
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 40 , Issue 1 , March 1992 , pp. 1 - 6
Copyright
Copyright © 1992 by the Weed Science Society of America 

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