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Effect of Sethoxydim and Haloxyfop on Acetyl-Coenzyme a Carboxylase Activity in Festuca Species

Published online by Cambridge University Press:  12 June 2017

David E. Stoltenberg ,
John W. Gronwald ,
Donald L. Wyse ,
James D. Burton ,
David A. Somers  and
Burle G. Gengenbach
David E. Stoltenberg
Affiliation:
Dep. Agron. and Plant Genetics, Univ. Minnesota, St. Paul, MN 55108
John W. Gronwald
Affiliation:
Dep. Agron. and Plant Genetics, Univ. Minnesota, St. Paul, MN 55108
Donald L. Wyse
Affiliation:
Dep. Agron. and Plant Genetics, Univ. Minnesota, St. Paul, MN 55108
James D. Burton
Affiliation:
Dep. Agron. and Plant Genetics, Univ. Minnesota, St. Paul, MN 55108
David A. Somers
Affiliation:
Dep. Agron. and Plant Genetics, Univ. Minnesota, St. Paul, MN 55108
Burle G. Gengenbach
Affiliation:
Dep. Agron. and Plant Genetics, Univ. Minnesota, St. Paul, MN 55108
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Abstract

In greenhouse studies, the calculated I50 (herbicide application resulting in 50% inhibition of shoot regrowth) in tall fescue was approximately 0.004 kg/ha for both sethoxydim and haloxyfop. In red fescue, the I50 for sethoxydim and haloxyfop was 9.4 kg/ha and 0.04 kg/ha, respectively. As measured in crude cell extracts of tall fescue, incorporation of 14C-acetyl-coenzyme A into fatty acids was inhibited 62 and 71% by 10 μM sethoxydim and 10 μM haloxyfop, respectively. In red fescue, 10 μM haloxyfop inhibited 14C-acetyl-CoA incorporation into fatty acids by 29%, whereas 10 μM sethoxydim had no effect. The I50 for inhibition of acetyl-coenzyme A carboxylase activity in tall fescue with sethoxydim and haloxyfop was 6.9 and 5.8 μM, respectively. In red fescue the I50 for haloxyfop was 118 μM. Sethoxydim concentrations as high as 1 mM had little effect on acetyl-coenzyme A carboxylase activity in red fescue. These results suggest that acetyl-coenzyme A carboxylase is a sensitive site of action for sethoxydim and haloxyfop in tall fescue, and that tolerance to these herbicides in red fescue is due to the presence of a more tolerant form of the enzyme.

Keywords

Red fescue, Festuca rubra L. ♯3 FESRU, ‘Pennlawn’tall fescue, Festuca arundinacea Schreb. ♯ FESAR, ‘Kentucky 31′sethoxydim, 2-[1-(ethoxyimino)butyl]-5-[2-(ethylthio)propyl]-3-hydroxy-2-cyclohexen-1-onehaloxyfop, 2-[4-[[3-chloro-5-(trifluoromethyl)-2-pyridinyl]oxy]-phenoxy]propanoic acidacetyl-coenzyme A carboxylase (EC 6.4.1.2)Fatty acid biosynthesiscyclohexanedionearyloxyphenoxypropionateFESARFESRU
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 37 , Issue 4 , July 1989 , pp. 512 - 516
Copyright
Copyright © 1989 by the Weed Science Society of America 

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References

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