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Effects of Clomazone on IPP Isomerase and Prenyl Transferase Activities in Cell Suspension Cultures and Cotyledons of Solanaceous Species

Published online by Cambridge University Press:  12 June 2017

Jon E. Scott ,
Leslie A. Weston ,
Joseph Chappell  and
Kathleen Hanley
Jon E. Scott
Affiliation:
Dep. of Hortic. and Landscape Arch., Univ. Kentucky, Lexington, KY 40546
Leslie A. Weston
Affiliation:
Dep. of Agron., Univ. Kentucky, Lexington, KY 40546
Joseph Chappell
Affiliation:
Dep. of Agron., Univ. Kentucky, Lexington, KY 40546
Kathleen Hanley
Affiliation:
Biosource Genetics Co., Bowman Gray Tech. Ctr., Bldg. 611, Winston Salem, NC 27102
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Abstract

Laboratory assays were conducted to determine the sensitivity of tomato and tobacco cell suspension cultures and tomato and pepper cotyledons to clomazone. A comparison of fresh weight and carotenoid content indicated up to a three-fold difference between the clomazone-tolerant tobacco and clomazone-susceptible tomato cell suspension cultures. In contrast, an approximate 60-fold difference between the tolerant pepper and susceptible tomato cotyledons was observed when total chlorophyll and carotenoid contents were measured. The effect of clomazone and its possible metabolites on in vivo and in vitro extractable IPP isomerase (EC 5.3.3.2) and prenyltransferase (EC 2.5.1.29) activity was investigated. There was no clear inhibitory effect of clomazone or possible clomazone metabolites upon enzyme activity in tomato or tobacco cell suspension cultures or on light or dark grown tomato or pepper cotyledons. No specific enzymatic target site of clomazone was identified in correlation with the reduction in total chlorophyll or carotenoid content.

Keywords

Clomazone, 2-[(2-chlorophenyl)methyl]-4,4-dimethyl-3-isoxazolidinonepepper, Capsicum annum L. cv Yolo Wonder L.tobacco, Nicotiana tabacum L. ‘KY-14’tomato, Lycopersicon esculentum Mill. ‘Niagara.’CarotenoidschlorophylldimethazoneFMC 57020selectivity
Type
Physiology, Chemistry, and Biochemostry
Information
Weed Science , Volume 42 , Issue 4 , December 1994 , pp. 509 - 516
Copyright
Copyright © 1994 by the Weed Science Society of America 

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References

Literature Cited

1. Artus, N. N., Ryberg, M., Lindsten, A., Ryberg, H., and Sundqvist, C. 1992. The shibata shift and the transformation of etioplasts to chloroplasts in wheat with clomazone (FMC 57020) and amiprophos-methyl (Tokunol M). Plant Physiol. 98:253–263.Google Scholar
2. Bradford, M. 1976. A rapid and sensitive method for quantitation of micro-gram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248–254.Google Scholar
3. Chappell, J. and Nable, R. 1987. Induction of sesquiterpenoid biosynthesis in tobacco cell suspension cultures by fungal elicitor. Plant Physiol. 85:469–473.Google Scholar
4. Croteau, R. 1992. Clomazone does not inhibit the conversion of isopentenyl pyrophosphate to geranyl, farnesyl, or geranylgeranyl pyrophosphate in-vitro. Plant Physiol. 98:1515–1517.CrossRefGoogle ScholarPubMed
5. Dudley, M. W., Dueber, M. T., and West, C. A. 1986. Biosynthesis of the macrocyclic diterpene casbene in castor bean (Ricinus communis L.) seedlings. Changes in enzyme levels induced by fungal infection and intracellular localization of the pathway. Plant Physiol. 81:335–342.Google Scholar
6. Dudley, M. W., Green, T. R., and West, C. A. 1986. Biosynthesis of the macrocyclic diterpene casbene in castor bean (Riciunus communis L.) seedlings. The purification and properties of farnesyl transferase from elicited seedlings. Plant Physiol. 81:343–348.Google Scholar
7. Duke, S. O. and Kenyon, W. H. 1986. Effects of dimethazone (FMC 57020) on chloroplast development. II. Pigment synthesis and photosynthetic function in cowpea (Vigna unguiculata L.) primary leaves. Pestic. Biochem. Physiol. 25:11–18.CrossRefGoogle Scholar
8. Duke, S. O., Kenyon, W. H., and Paul, R. N. 1985. FMC 57020 effects on chloroplast development in pitted morningglory (Ipomoea lacunosa) cotyledons. Weed Sci. 33:786–794.Google Scholar
9. Duke, S. O., Paul, R. N., Becerril, J. M., and Schmidt, J. H. 1991. Clomazone causes accumulation of sesquiterpenoids in cotton (Gossypium hirsutum L.). Weed Sci. 39:339–346.CrossRefGoogle Scholar
10. Feron, G., Clastre, M., and Ambid, C. 1990. Prenyltransferase compartmentation in cells of (Vitis vinifera) cultivated in vitro. Fed. Eur. Biochem. Soc. 271(1, 2):236–238.Google Scholar
11. Hanley, K. H., Vogeli, U., and Chappell, J. 1992. A study of the isoprenoid pathway in elicitor-treated tobacco cell suspension cultures. In Petroski, R. J., ed., Biosynthesis and metabolism of secondary metabolite natural products, Plenum Press, New York (in press).Google Scholar
12. Heide, L. 1988. Geranylpyrophosphate synthase from cell cultures of (Lithospermum erythrorhizon). Fed. Eur. Biochem. Soc. 237(1, 2): 159–162.Google Scholar
13. Inskeep, W. P. and Bloom, P. R. 1985. Extinction coefficients of chlorophyll a and b in N,N-dimethylformamide and 80% acetone. Plant Physiol. 77:483–485.CrossRefGoogle Scholar
14. Koopmans, L. H. 1981. An introduction to contemporary statistics. Duxbury Press, Boston, MS, p. 89.Google Scholar
15. Lee, C.-Y. 1982. Glucose-6-phosphate dehydrogenase from mouse. Methods Enzymol. 89:252–257.Google Scholar
16. Lützow, M. and Beyer, P. 1988. The isopentenyl-diposphate Δ-isomerase and its relation to the phytoene synthase complex in daffodil chromoplasts. Biochim. Biophys. Acta 959:118–126.Google Scholar
17. Lutzow, M., Beyer, P., and Kleinig, H. 1990. The herbicide Command does not inhibit the prenyl diphosphate-forming enzymes in plastids. Z. Naturforsch. 45c:856–858.Google Scholar
18. Mayfield, S. P., Nelson, T., Taylor, W. C., and Malkin, R. 1986. Carotenoid synthesis and pleiotropic effects in carotenoid-deficient seedling of maize. Planta 169:23–32.Google Scholar
19. Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497.Google Scholar
20. Norman, M. A., Liebl, R. A., and Widholm, J. M. 1990. Uptake and metabolism of clomazone in tolerant-soybean and susceptible-cotton photomixotrophic cell suspension cultures. Plant Physiol. 92:777–784.CrossRefGoogle ScholarPubMed
21. Norman, M. A., Liebl, R. A., and Widholm, J. M. 1990. Site of clomazone action in tolerant-soybean and susceptible-cotton photomixotrophic cell suspension cultures. Plant Physiol. 94:704–709.Google Scholar
22. Poulter, C. D. and Rilling, H. C. 1981. Prenyl transferases and isomerase. Pages 161–224 in Porter, J. W. and Spurgeon, L., eds. Biosynthesis of isoprenoid compounds, Vol I. John Wiley and Sons, New York.Google Scholar
23. Satterwhite, D. M. 1985. Isopentenyldiphosphate Δ-isomerase. Methods Enzymol. 110:92–99.Google Scholar
24. Sandmann, G. and Büger, P. 1986. Interference of dimethazone with formation of terpenoid compounds. Z. Naturforsch. 41c:729–732.Google Scholar
25. Sandmann, G. and Büger, P. 1987. Interconversion of prenyl pyrophosphates and subsequent reactions in the presence of FMC 57020. Z. Naturforsch. 42c:803–807.CrossRefGoogle Scholar
26. Scott, J. E. and Weston, L. A. 1992. Cole crop (Brassica oleracea) tolerance to clomazone. Weed Sci. 40:7–11.Google Scholar
27. Vencill, W. K., Hatzios, K. K., and Wilson, H. P. 1990. Absorption, translocation, and metabolism of 14C-clomazone in soybean (Glycine max) and three Amaranthus weed species. J. Plant Growth Regul. 9:127–132.CrossRefGoogle Scholar
28. Weimer, M. R., Balke, N. E., and Buhler, D. D. 1992. Herbicide clomazone does not inhibit in-vitro geranylgeranyl synthesis from mevalonate. Plant Physiol. 98:427–432.Google Scholar
29. Weimer, M. R., Buhler, D. D., and Balke, N. E. 1991. Clomazone selectivity: absence of differential uptake, translocation, or detoxication. Weed Sci. 39:529–534.CrossRefGoogle Scholar
30. Weston, L. A. and Barrett, M. 1989. Tolerance of tomato (Lycopersicon esculentum) and bell pepper (Capsicum annum) to clomazone. Weed Sci. 37:285–289.Google Scholar
31. Weston, L. A., White, C. D., and Harmon, R. 1990. Differential tolerance of velvetleaf, jimsonweed, and morningglory to clomazone. Abstr. Weed Sci. Soc. Am. 30:83.Google Scholar