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First report of molecular basis of resistance to imazethapyr in common lambsquarters (Chenopodium album)

Published online by Cambridge University Press:  29 October 2019

Zhaofeng Huang Open the ORCID record for Zhaofeng Huang [Opens in a new window] ,
Xinxin Zhou ,
Chaoxian Zhang ,
Cuilan Jiang ,
Hongjuan Huang  and
Shouhui Wei
Zhaofeng Huang
Affiliation:
Associate Professor, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
Xinxin Zhou
Affiliation:
Associate Professor, Institute for the Control of Agrochemicals, Ministry of Agriculture and Rural Affairs, Beijing, China
Chaoxian Zhang
Affiliation:
Professor, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
Cuilan Jiang
Affiliation:
Assistant Professor, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
Hongjuan Huang*
Affiliation:
Associate Professor, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
Shouhui Wei*
Affiliation:
Associate Professor, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China
*
Authors for correspondence: Shouhui Wei and Hongjuan Huang, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. (Emails: shwei@ippcaas.cn, hjhuang@ippcaas.cn)
Authors for correspondence: Shouhui Wei and Hongjuan Huang, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. (Emails: shwei@ippcaas.cn, hjhuang@ippcaas.cn)
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Abstract

Common lambsquarters (Chenopodium album L.) is one of the most troublesome weeds in soybean [Glycine max (L.) Merr.] and corn (Zea mays L.) fields in northeast China. In 2017, a C. album population that survived imazethapyr at the recommended field rate was collected from a soybean field in Heilongjiang Province in China. Experiments were conducted to determine the basis of resistance to imazethapyr and investigate the herbicide-resistance pattern in C. album. Dose–response tests showed that the resistant population (R) displayed high resistance to imazethapyr (20-fold) compared with the susceptible population (S). An in vitro acetolactate synthase (ALS) activity assay indicated that the ALS of the R population was resistant to imazethapyr compared with the ALS of the S population. Sequence analysis of the ALS gene revealed that the GCA was replaced by ACA at amino acid position 122, which resulted in an alanine to threonine substitution (Ala-122-Thr) in the R population. The R population displayed cross-resistance to thifensulfuron-methyl and flumetsulam but susceptibility to bispyribac-sodium, flucarbazone, glyphosate, mesotrione, and fomesafen. These results confirmed that the basis of imazethapyr resistance in C. album was conferred by the Ala-122-Thr substitution in the ALS enzyme. This is the first report of the target-site basis of ALS-inhibiting herbicide resistance in C. album.

Keywords

ALS enzymeherbicideherbicide resistancetarget sitemutation
Type
Research Article
Information
Weed Science , Volume 68 , Issue 1 , January 2020 , pp. 63 - 68
Copyright
© Weed Science Society of America, 2019

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Footnotes

Associate Editor: Franck E. Dayan, Colorado State University

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