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Glufosinate metabolism in excised shoots and leaves of twenty plant species

Published online by Cambridge University Press:  20 January 2017

Carin Jansen ,
Ingolf Schuphan  and
Burkhard Schmidt
Carin Jansen
Affiliation:
Department of Biology V (Ecology/Ecotoxicology/Ecochemistry), Technical University of Aachen, Worringer Weg 1, D-52056 Aachen, Germany
Ingolf Schuphan
Affiliation:
Department of Biology V (Ecology/Ecotoxicology/Ecochemistry), Technical University of Aachen, Worringer Weg 1, D-52056 Aachen, Germany
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Abstract

The metabolism of the herbicide 14C-glufosinate (GA) was studied in excised shoots and leaves of 20 weed and nonweed species; GA was fed through the xylem. After 24 or 48 h of incubation, the plant material was examined for phytotoxic symptoms, analyzed by autoradiography, and extracted. The extract was cleaned up and analyzed by high-performance liquid chromatography. GA and its metabolites were identified by cochromatography with authentic 14C-labelled reference compounds. 14C-GA was rapidly absorbed by the excised plant parts. Most of the radioactivity (91.3 to 99.7%) in the shoots and leaves was extractable. The main metabolite observed with all species was 3-(hydroxymethylphosphinyl)propionic acid (MPP); lower amounts of 2-hydroxy-4-(hydroxymethylphosphinyl)butanoic acid (MHB) were also found in 14 species. Metabolic rates of GA varied considerably ranging between 13.1 and 2,836.5 ng GA h−1 mg−1 protein. The species with the highest rates of metabolism of GA were Galium verum (2,836.5 ng GA h−1 mg−1 protein), Lythrum hyssopifolia (846.0 ng GA h−1 mg−1 protein), and Digitalis purpurea (494.8 ng GA h−1 mg−1 protein). The mean value across all species was 275.9 ng GA h−1 mg−1 protein.

Keywords

GA, glufosinate (racemic mixture)DGA, D-glufosinateLGA, L-glufosinateMPA, 2-(hydroxymethylphosphinyl)acetic acidMPB, 4-(hydroxymethylphosphinyl)butanoic acidMPP, 3-(hydroxymethylphosphinyl)propionic acidPPO, 2-oxo-4-(hydroxymethylphosphinyl) butanoic acidAgrostemma githago L. AGOGI, corn cockleAvena fatua L. AVEFA, wild oatDigitalis purpurea L. DIKPU, foxgloveFumaria officinalis L. FUMOF, fumitoryGalium verum L. GALVE, yellow bedstrawGeum urbanum L. GEUUR, common avensMalva sylvestris L. MALSI, high mallowAutoradiographyexcised shoots and leavesAGOGIALOMYAMAREAVEFACMAALCHQMACHEALDIKPUFUMOFGALAPGALVEGEUURGERROLOLPELYTHYMALSIMATCHPOAPRSINARDAUCA
Type
Research Article
Information
Weed Science , Volume 48 , Issue 3 , June 2000 , pp. 319 - 326
Copyright
Copyright © Weed Science Society of America 

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