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Isozyme and RAPD variation among and within hemp dogbane (Apocynum cannabinum) populations

Published online by Cambridge University Press:  12 June 2017

Corey V. Ransom ,
David S. Douches  and
James J. Kells
Corey V. Ransom
Affiliation:
Department of Crop and Soil Sciences, Michigan State University, East Lansing, MI 48824-1325
David S. Douches
Affiliation:
Department of Crop and Soil Sciences, Michigan State University, East Lansing, MI 48824-1325
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Abstract

Clonal individuals from 16 hemp dogbane populations with phenotypic variation were analyzed using isozyme and randomly amplified polymorphic DNA (RAPD) analysis. Plants originated from populations in Michigan and Illinois. Three known Apocynum species, spreading dogbane, hemp dogbane, and prairie dogbane, were evaluated. Genetic distance among populations was more pronounced with isozyme analysis compared to RAPD analysis. The combined isozyme and RAPD analysis data separated spreading dogbane from all other plants analyzed. Genetic variation was present among the 16 hemp dogbane populations, but was less than expected based on the phenotypic variation present among the collections. The short genetic distance between the 16 hemp dogbane collections and the three Apocynum species suggests that variation among populations of hemp dogbane may be from outcrossing with other closely related Apocynum species. Isozyme and RAPD analyses were also conducted on plants from two populations in Michigan to determine the level of genetic variation among plants within the same population. Genetic analysis revealed that one population was entirely clonal, while the other population was a mixture of clonal and segregating plants.

Keywords

Hemp dogbane, Apocynum cannabinum L. APPCAprairie dogbane, Apocynum sibericum Jacq. APCVEspreading dogbane, Apocynum androsaemifolium L. APCANGenetic variationpolymorphismisozyme analysisRAPD analysisAPPCAAPCVEAPCAN
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 46 , Issue 4 , August 1998 , pp. 408 - 413
Copyright
Copyright © 1998 by the Weed Science Society of America 

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Footnotes

Current address: Malheur Experiment Station, Oregon State University, 595 Onion Avenue, Ontario, OR 97914

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