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Microarray and Growth Analyses Identify Differences and Similarities of Early Corn Response to Weeds, Shade, and Nitrogen Stress

Published online by Cambridge University Press:  20 January 2017

Janet Moriles ,
Stephanie Hansen ,
David P. Horvath ,
Graig Reicks ,
David E. Clay  and
Sharon A. Clay
Janet Moriles
Affiliation:
Department of Plant Science, South Dakota State University, 1110 Rotunda Lane, Brookings, SD 57007
Stephanie Hansen
Affiliation:
Department of Plant Science, South Dakota State University, 1110 Rotunda Lane, Brookings, SD 57007
David P. Horvath
Affiliation:
USDA-ARS, 1605 Albrecht Blvd. N, Fargo, ND, 58102-2765
Graig Reicks
Affiliation:
Department of Plant Science, South Dakota State University, 1110 Rotunda Lane, Brookings, SD 57007
David E. Clay
Affiliation:
Department of Plant Science, South Dakota State University, 1110 Rotunda Lane, Brookings, SD 57007
Sharon A. Clay*
Affiliation:
Department of Plant Science, South Dakota State University, 1110 Rotunda Lane, Brookings, SD 57007
*
Corresponding author's E-mail: sharon.clay@sdstate.edu
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Abstract

Weed interference with crop growth is often attributed to water, nutrient, or light competition; however, specific physiological responses to these stresses are not well described. This study's objective was to compare growth, yield, and gene expression responses of corn to nitrogen (N), low light (40% shade), and weed stresses. Corn vegetative parameters from V2 to V12 stages, yield parameters, and gene expression using transcriptome (2008) and quantitative polymerase chain reaction (qPCR) (2008/09) analyses at V8 were compared among the stresses and with nonstressed corn. N stress did not affect vegetative parameters, although grain yield was reduced by 40% compared with nonstressed plants. Shade, present until V2, reduced biomass and leaf area > 50% at V2, and recovering plants remained smaller than nonstressed plants at V12. However, grain yields of shade-stressed and nonstressed plants were similar, unless shade remained until V8. Weed stress reduced corn growth and yield in 2008 when weeds remained until V6. In 2009, weed stress until V2 reduced corn vegetative growth, but yield reductions occurred only if weed stress remained until V6 or later. Principle component analysis of differentially expressed genes indicated that shade and weed stress had more similar gene expression patterns to each other than they did to nonstressed or N-stressed tissues. However, corn grown in N-stressed conditions shared 252 differentially expressed genes with weed-stressed plants. Ontologies associated with light/photosynthesis, energy conversion, and signaling were down-regulated in response to all three stresses. Shade and weed stress clustered most tightly together, based on gene expression, but shared only three ontologies, O-METHYLTRANSFERASE activity (lignification processes), POLY(U)-BINDING activity (posttranscriptional gene regulation), and stomatal movement. Based on morphologic and genomic observations, weed stress to corn was not explained by individual effects of N or light stress. Therefore, we hypothesize that these stresses share limited signaling mechanisms.

Keywords

Corn, Zea mays LWeed competitiongenomicstranscriptome analysiscritical weed free period
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 60 , Issue 2 , June 2012 , pp. 158 - 166
Copyright
Copyright © Weed Science Society of America 

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