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Molecular Responses of Sorghum to Purple Witchweed (Striga hermonthica) Parasitism

Published online by Cambridge University Press:  20 January 2017

Yukihiro Hiraoka  and
Yukihiro Sugimoto
Yukihiro Hiraoka
Affiliation:
Graduate School of Science and Technology, Kobe University, Rokkodai, Nada, Kobe 657-8501, Japan
Yukihiro Sugimoto*
Affiliation:
Graduate School of Science and Technology, Kobe University, Rokkodai, Nada, Kobe 657-8501, Japan
*
Corresponding author's E-mail: yukihiro@kobe-u.ac.jp
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Abstract

Sorghum genes responsive to purple witchweed parasitism were isolated, and their expression was analyzed. Using the suppression-subtractive hybridization strategy, 30 genes that were up-regulated in response to purple witchweed parasitism were isolated from the roots of a susceptible sorghum cultivar ‘Abu 70’. The changes in the expression of each gene were investigated in the roots and leaves of the sorghum cultivars ‘Wad Ahmed’ and ‘Tabat’ were parasitized by purple witchweed. Tabat is more susceptible and Wad Ahmed is less susceptible to purple witchweed than Abu 70. Further, the changes in the gene expression and host susceptibility to purple witchweed were studied in the roots of the three sorghum cultivars after treatment with salicylic acid (SA) or methyl jasmonate (MeJA). Purple witchweed parasitism induced jasmonic acid (JA)-responsive genes and suppressed SA-responsive genes in the roots of the cultivars Abu 70 and Tabat. In contrast, purple witchweed parasitism in the less-susceptible cultivar Wad Ahmed induced SA-responsive genes and induced JA-responsive genes to a small extent. SA, but not MeJA, decreased the susceptibility of all the sorghum cultivars to purple witchweed. Systemic expression of a few genes was observed in the leaves of the purple witchweed–parasitized sorghum cultivars.

Keywords

Purple witchweed, Striga hermonthica (Del) Benth. STRHEsorghum, Sorghum bicolor (L.) Moench SORVUParasitic weedsuppression subtractive hybridizationhost response
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 56 , Issue 3 , June 2008 , pp. 356 - 363
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

Agre, P., Bonhivers, M., and Borgnia, M. J. 1998. The aquaporins, blueprints for cellular plumbing systems. J. Biol. Chem. 273:14659–14662.CrossRefGoogle ScholarPubMed
Akiyama, K., Matsuzaki, K., and Hayashi, H. 2005. Plant sesquiterpenes induce hyphal branching in arbuscular mycorrhizal fungi. Nature. 435:824–827.Google Scholar
Berner, D. K., Kling, J. G., and Singh, B. B. 1995. Striga research and control. Plant Dis. 79:652–660.Google Scholar
Cai, T., Babiker, A. G., and Butler, L. G. 1993. Morphological response of witchweed (Striga asiatica) to in vitro culture. J. Exp. Bot. 44:1377–1384.Google Scholar
Cheong, Y. H., Chang, H. S., Gupta, R., Wang, X., Zhu, T., and Luan, S. 2002. Transcriptional profiling reveals novel interactions between wounding, pathogen, abiotic stress, and hormonal responses in Arabidopsis . Plant Physiol. 129:661–677.Google Scholar
Diatchenko, L., Lau, Y-F. C., Campbell, A. P., Chenchik, A., Moqadam, F., Huang, B., Lukyanov, S., Lukyanov, K., Gurskaya, N., Sverdlov, E., and Siebert, P. D. 1996. Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proc. Natl. Acad. Sci. U. S. A. 93:6025–6030.Google Scholar
Garbarino, J. E., Rockhold, D. R., and Belknap, W. R. 1992. Expression of stress-responsive ubiquitin genes in potato tubers. Plant Mol. Biol. 20:235–244.CrossRefGoogle ScholarPubMed
Gowda, B. S., Riopel, J. L., and Timko, M. P. 1999. NRSA-1: a resistance gene homolog expressed in roots of non-host plants following parasitism by Striga asiatica (witchweed). Plant J. 20:217–230.Google Scholar
Gurney, A. L., Slate, J., Press, M. C., and Scholes, J. D. 2006. A novel form of resistance in rice to the angiosperm parasite Striga hermonthica . New Phytol. 169:199–208.CrossRefGoogle Scholar
Hamada, T., Nishiuchi, T., Kodama, H., Nishimura, M., and Iba, K. 1996. cDNA cloning of a wounding-inducible gene encoding a plastid ω-3 fatty acid desaturase from tobacco. Plant Cell Physiol. 37:606–611.Google Scholar
Hamilton, E. W. and Coleman, J. S. 2001. Heat-shock proteins are induced in unstressed leaves of Nicotiana attenuata (Solanaceae) when distant leaves are stressed. Am. J. Bot. 88:950–955.Google Scholar
Hipskind, J. D., Hanau, R., Leite, B., and Nicholson, B. L. 1990. Phytoalexin accumulation in sorghum: identification of an apigeninidin acyl ester. Physiol. Mol. Plant Pathol. 36:381–396.CrossRefGoogle Scholar
Hochstrasser, M. 1996. Ubiquitin-dependent protein degradation. Annu. Rev. Genet. 1996 30:405–439.Google Scholar
Kim, K. C., Fan, B., and Chen, Z. 2006. Pathogen-induced Arabidopsis WRKY7 is a transcriptional repressor and enhances plant susceptibility to Pseudomonas syringae . Plant Physiol. 142:1180–1192.Google Scholar
Krishna, P. and Gloor, G. 2001. The hsp90 family of proteins in Arabidopsis thaliana . Cell Stress and Chaperones. 6:238–246.Google Scholar
Kusumoto, D., Goldwasser, Y., Xie, X., Yoneyama, K., Takeuchi, Y., and Yoneyama, K. 2007. Resistance of red clover (Trifolium pratense) to the root parasitic plant Orobanche minor is activated by salicylate but not by jasmonate. Ann. Bot. 100:537–544.Google Scholar
Liechti, R. and Farmer, E. E. 2002. The jasmonate pathway. Science. 296:1649–1650.Google Scholar
Livak, K. J. and Schmittgen, T. D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔC T method. Methods. 25:402–408.Google Scholar
Mason, H. S. and Mullet, J. E. 1990. Expression of two soybean vegetative storage protein genes during development and in response to water deficit, wounding, and jasmonic acid. Plant Cell. 2:569–579.Google Scholar
Matusova, R., Rani, K., Verstappen, F. W. A., Franssen, M. C. R., Beale, M. H., and Bouwmeester, H. J. 2005. The strigolactone germination stimulants of the plant-parasitic Striga and Orobanche spp. are derived from the carotenoid pathway. Plant Physiol. 139:920–934.Google Scholar
Mohamed, A., Ellicott, A., Housley, T. L., and Ejeta, G. 2003. Hypersensitive response to Striga infection in sorghum. Crop Sci. 43:1320–1324.Google Scholar
Nemchenko, A., Kunze, S., Feussner, I., and Kolomiets, M. 2006. Duplicate maize 13-lipoxygenase genes are differentially regulated by circadian rhythm, cold stress, wounding, pathogen infection, and hormonal treatments. J. Exp. Bot. 57:3767–3779.Google Scholar
Nicholson, R. L., Kollipara, S. S., Vincent, J. R., Lyons, P. C., and Cadena-Gomez, G. 1987. Phytoalexin synthesis by the sorghum mesocotyl in response to infection by pathogenic and non pathogenic fungi. Proc. Natl. Acad. Sci. U. S. A. 84:5520–5524.Google Scholar
Niki, T., Mitsuhara, I., Seo, S., Ohtsubo, N., and Ohashi, Y. 1998. Antagonistic effect of salicylic acid and jasmonic acid on the expression of pathogenesis-related (PR) protein genes in wounded mature tobacco leaves. Plant Cell Physiol. 39:500–507.Google Scholar
Sachs, J. V. 1975. Experimental methods for the investigation of plant nutrient requirements. Pages 31–69. In Hewitt, E. J. and Smith, T. A. Plant Mineral Nutrition. London English Universities Press.Google Scholar
Seki, M., Narusaka, M., Abe, H., Kasuga, M., Yamaguchi-Shinozaki, K., Carninci, P., Hayashizaki, Y., and Shinozaki, K. 2001. Monitoring the expression pattern of 1300 Arabidopsis genes under drought and cold stresses by using a full-length cDNA microarray. Plant Cell. 13:61–72.Google Scholar
Sugimoto, Y., Ali, A. M., Yabuta, S., Kinoshita, H., Inanaga, S., and Itai, A. 2003. Germination strategy of Striga hermonthica involves regulation of ethylene biosynthesis. Physiol. Plant. 119:137–145.Google Scholar
Takahashi, R. and Shimosaka, E. 1997. cDNA sequence analysis and expression of two cdd-regulated genes in soybean. Plant Sci. 123:93–104.Google Scholar
Torres, M. J., Tomilov, A. A., Tomilova, N., Reagan, R. L., and Yoder, J. I. 2005. Pscroph, a parasitic plant EST database enriched for parasite associated transcripts. BMC Plant Biol. 5:1–9.Google Scholar
Vieira-Dos-Santos, C., Delavault, P., Letousey, P., and Thalouarn, P. 2003. Defense gene expression analysis of Arabidopsis thaliana parasitized by Orobanche ramosa . Phytopathology. 93:451–457.Google Scholar
Vogel, J. P., Raab, T. K., Schiff, C., and Somerville, S. C. 2002. PMR6, a pectate lyase-like gene required for powdery mildew susceptibility in Arabidopsis . Plant Cell. 14:2095–2106.Google Scholar