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Multiple resistance to imazethapyr and atrazine in Powell amaranth (Amaranthus powellii)

Published online by Cambridge University Press:  20 January 2017

R. Shane Diebold ,
Kristen E. McNaughton ,
Elizabeth A. Lee  and
François J. Tardif
R. Shane Diebold
Affiliation:
Department of Plant Agriculture, University of Guelph, Guelph, Ontario, Canada N1G 2W1
Kristen E. McNaughton
Affiliation:
Department of Plant Agriculture, University of Guelph, Guelph, Ontario, Canada N1G 2W1
Elizabeth A. Lee
Affiliation:
Department of Plant Agriculture, University of Guelph, Guelph, Ontario, Canada N1G 2W1
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Abstract

Multiple-herbicide resistance represents an added weed management challenge to growers as it can considerably reduce their options for weed control. The widespread nature of triazine resistance in Ontario coupled with the more recent appearance of resistance to ALS inhibitors in Amaranthus species warranted documenting biotypes with multiple resistance. A collection of Powell amaranth and redroot pigweed biotypes that had previously been characterized for resistance to ALS inhibitors was therefore screened with atrazine. Dose–response analysis with atrazine and imazethapyr was also conducted. High-level resistance to imazethapyr and atrazine was determined in a Powell amaranth biotype from Perth County, Ontario. This biotype had a > 1,860-fold and 109-fold resistance to atrazine and imazethapyr, respectively. Sequence analysis was conducted for the psbA and ALS genes that code for the target sites of the triazines and imidazolinones, respectively. A mutation in the psbA gene was identified that coded for an amino acid substitution of glycine for serine at residue 264 of the D1 protein. This mutation is the most likely cause for triazine resistance in this biotype. Similarly, a nucleotide substitution was identified that codes for threonine in place of serine at position 652 of the ALS protein. This mutation in the ALS gene has only been observed previously in laboratory-selected mutants of arabidopsis and tobacco and is known to endow resistance to imidazolinones in plants. It is concluded that multiple resistance in this Powell amaranth biotype is due to the presence of altered target sites for triazine and imidazolinone herbicides.

Keywords

AtrazineimazethapyrPowell amaranth, Amaranthus powellii S. Wats. AMAPOredroot pigweed, Amaranthus retroflexus L. AMAREArabidopsis thaliana (L.) Heynh. Arabidopsistobacco, Nicotiana tabacum L.Herbicide resistanceacetolactate synthasedose–responsepsbA geneALS genetriazinesimidazolinonestarget site
Type
Research Article
Information
Weed Science , Volume 51 , Issue 3 , June 2003 , pp. 312 - 318
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

Bernasconi, P., Woodworth, A. R., Rosen, B. A., Subramanian, M. V., and Siehl, D. L. 1995. A naturally occurring point mutation confers broad range tolerance to herbicides that target acetolactate synthase. J. Biol. Chem. 270:17 381–17 385.Google Scholar
Bernasconi, P., Woodworth, A. R., Rosen, B. A., Subramanian, M. V., and Siehl, D. L. 1996. A naturally occurring point mutation confers broad range tolerance to herbicides that target acetolactate synthase. J. Biol. Chem. 271:13,925.Google Scholar
Burnet, M.W.M., Hart, Q., Holtum, J.A.M., and Powles, S. B. 1994. Resistance to nine herbicide classes in a population of rigid ryegrass (Lolium rigidum). Weed Sci. 42:369–377.Google Scholar
Chong, C. K. and Choi, J. D. 2000. Amino acid residues conferring herbicide tolerance in tobacco acetolactate synthase. Biochem. Biophys. Res. Commun. 279:462–467.CrossRefGoogle ScholarPubMed
Devine, M. D. and Eberlein, C. V. 1997. Physiological, biochemical, and molecular aspects of herbicide resistance based on altered target sites. Pages 159–185 In Roe, R. M., Burton, J. D., and Kuhr, R. J., eds. Herbicide Activity: Toxicology, Biochemistry, and Molecular Biology. Burke, VA: IOS Press.Google Scholar
Ferguson, G. M., Hamill, A. S., and Tardif, F. J. 2001. ALS-inhibitor resistance in populations of Amaranthus powellii and Amaranthus retroflexus . Weed Sci. 49:448–453.Google Scholar
Foes, M. J., Liu, L., Tranel, P. J., Wax, L. M., and Stoller, E. W. 1998. A biotype of common waterhemp (Amaranthus rudis) resistant to triazine and ALS herbicides. Weed Sci. 46:514–520.CrossRefGoogle Scholar
Foes, M. J., Liu, L., Vigue, G., Stoller, E. W., Wax, L. M., and Tranel, P. J. 1999. A kochia (Kochia scoparia) biotype resistant to triazine and ALS-inhibiting herbicides. Weed Sci. 47:20–27.Google Scholar
Gronwald, J. W. 1994. Resistance to photosystem II inhibiting herbicides. Pages 83–139 In Powles, S. B. and Holtum, J.A.M., eds. Herbicide Resistance in Plants: Biology and Biochemistry. Boca Raton, FL: Lewis Publishers.Google Scholar
Hall, L. M., Holtum, J.A.M., and Powles, S. B. 1994. Mechanisms responsible for cross resistance and multiple resistance. Pages 243–261 In Powles, S. B. and Holtum, J.A.M., eds. Herbicide Resistance in Plants: Biology and Biochemistry. Boca Raton, FL: Lewis Publishers.Google Scholar
Heap, I. M. 1997. The occurrence of herbicide-resistant weeds worldwide. Pestic. Sci. 51:235–243.3.0.CO;2-N>CrossRefGoogle Scholar
Heap, I. M. 2001. The International Survey of Herbicide Resistant Weeds: Web page: http://www.weedscience.com. Accessed: July 4, 2001.Google Scholar
Lee, Y., Chang, A. K., and Duggleby, R. G. 1999. Effect of mutagenesis at serine 653 of Arabidopsis thaliana acetohydroxyacid synthase on the sensitivity to imidazolinone and sulfonylurea herbicides. FEBS Lett. 452:341–345.CrossRefGoogle ScholarPubMed
Masabni, J. G. and Zandstra, B. H. 1999. A serine-to-threonine mutation in linuron-resistant Portulaca oleracea . Weed Sci. 47:393–400.Google Scholar
Mengistu, L. W., Mueller-Warrant, G. W., Liston, A., and Barker, R. E. 2000. psbA Mutation (valine219 to isoleucine) in Poa annua resistant to metribuzin and diuron. Pest Manag. Sci. 56:209–217.Google Scholar
Morden, C. W. and Golden, S. S. 1989. psbA genes indicate common ancestry of prochlorophytes and chloroplasts. Nature. 337:382–385.Google Scholar
Patzoldt, W. L., Tranel, P. J., Alexander, A. L., and Schmitzer, P. R. 2001. A common ragweed population resistant to cloransulam-methyl. Weed Sci. 49:485–490.CrossRefGoogle Scholar
Preston, and Mallory-Smith. 2001. Biochemical mechanisms, inheritance, and molecular genetics of herbicide resistance in weeds. Pages 23–60 In Powles, S. B. and Shaner, D. L., eds. Herbicide Resistance and World Grains. Boca Raton, FL: CRC Press.Google Scholar
Saari, L. L., Cotterman, J. C., and Thill, D. C. 1994. Resistance to acetolactate synthase inhibiting herbicides. Pages 83–139 In Powles, S. B. and Holtum, J.A.M., eds. Herbicide Resistance in Plants: Biology and Biochemistry. Boca Raton, FL: Lewis Publishers.Google Scholar
[SAS] Statistical Analysis Systems. 1996. The SAS System for Windows. Release 6.12. Cary, NC: Statistical Analysis Systems Institute.Google Scholar
Seefeldt, S. S., Jensen, J. E., and Fuerst, E. P. 1995. Log-logistic analysis of herbicide dose-response relationships. Weed Technol. 9:218–227.Google Scholar
Sibony, M., Michel, A., Haas, H. U., Rubin, B., and Hurle, K. 2001. Sulfometuron-resistant Amaranthus retroflexus: cross-resistance and molecular basis for resistance to acetolactate synthase-inhibiting herbicides. Weed Res. 41:509–522.Google Scholar
Smeda, R. J. and Vaughn, K. C. 1997. Mechanisms of resistance to herbicides. Pages 79–123 In Ebing, W., ed. Chemistry of Plant Protection: Molecular Mechanisms of Resistance to Agrochemicals. Berlin: Springer-Verlag.Google Scholar
Stephenson, G. R., Dykstra, M. D., McLaren, R. D., and Hamill, A. S. 1990. Agronomic practices influencing triazine-resistant weed distribution in Ontario. Weed Technol. 4:199–207.Google Scholar
Weaver, S. E. and McWilliams, E. L. 1980. The biology of Canadian weeds. 44. Amaranthus retroflexus L., A. powellii S. Wats., and A. hybridus L. Can. J. Plant Sci. 60:1215–1234.Google Scholar
Woodworth, A. R., Bernasconi, P., Subramanian, M. V., and Rosen, B. A. 1996. A second naturally occurring point mutation confers broad based tolerance to acetolactate synthase inhibitors. Plant Physiol. 111:S105.Google Scholar
Wright, T. R., Bascomb, N. F., Sturner, S. F., and Penner, D. 1998. Biochemical mechanism and molecular basis for ALS-inhibiting herbicide resistance in sugarbeet (Beta vulgaris) somatic cell selections. Weed Sci. 46:13–23.Google Scholar