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A Pro106 to Ala Substitution is Associated with Resistance to Glyphosate in Annual Bluegrass (Poa annua)

Published online by Cambridge University Press:  20 January 2017

Robert B. Cross ,
Lambert B. McCarty ,
Nishanth Tharayil ,
J. Scott McElroy ,
Shu Chen ,
Patrick E. McCullough ,
Brian A. Powell  and
William C. Bridges Jr.
Robert B. Cross
Affiliation:
School of Agriculture, Forestry, and Environmental Sciences, E-143 Poole Agriculture Center, Clemson University, Clemson, SC 29634
Lambert B. McCarty*
Affiliation:
School of Agriculture, Forestry, and Environmental Sciences, E-143 Poole Agriculture Center, Clemson University, Clemson, SC 29634
Nishanth Tharayil
Affiliation:
School of Agriculture, Forestry, and Environmental Sciences, E-143 Poole Agriculture Center, Clemson University, Clemson, SC 29634
J. Scott McElroy
Affiliation:
Department of Agronomy and Soils, 201 Funchess Hall, Auburn University, Auburn, AL 36849
Shu Chen
Affiliation:
Department of Agronomy and Soils, 201 Funchess Hall, Auburn University, Auburn, AL 36849
Patrick E. McCullough
Affiliation:
115 Redding Building, Department of Crop and Soil Sciences, University of Georgia, Griffin, GA 30223
Brian A. Powell
Affiliation:
Department of Environmental Engineering and Earth Sciences, 166 Rich Laboratory, Clemson University, Anderson, SC 29625
William C. Bridges Jr.
Affiliation:
Department of Mathematical Sciences, O-110 Martin Hall, Clemson University, Clemson, SC 29634
*
Corresponding author's E-mail: bmccrty@clemson.edu
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Abstract

Glyphosate is used in the transition zone to control annual bluegrass in fully dormant warm-season grasses. A suspected resistant (R) biotype of annual bluegrass was identified on a golf course in South Carolina after at least 10 consecutive years of glyphosate application. Greenhouse bioassays revealed the R biotype was 4.4-fold resistant to glyphosate compared with a standard susceptible (S) biotype. Further studies were conducted to investigate the mechanism conferring glyphosate resistance in the R biotype. Leaf discs of both biotypes accumulated shikimate in response to increasing glyphosate concentration, but the glyphosate concentration resulting in 50% EPSP synthase inhibition as a result of shikimate accumulation (I50) was 4.2-fold higher in the R biotype compared with the S biotype. At the whole plant level, similar levels of shikimate accumulation were observed between biotypes at 6 and 24 h after treatment (HAT) with glyphosate, but greater shikimate accumulation occurred in the S biotype at 72, 120, and 168 HAT. Shikimate levels decreased in the R biotype after 72 HAT. There were no differences in 14C-glyphosate absorption between biotypes. However, more 14C-glyphosate translocated out of the treated leaf in the R biotype and into root tissues over time compared with the S biotype. Partial sequencing of the EPSP synthase gene revealed a point mutation that resulted in an Ala substitution at Pro106. Although other mechanisms may contribute to glyphosate resistance, these results confirm a Pro106 to Ala substitution is associated with resistance to glyphosate in the R annual bluegrass biotype.

Keywords

GlyphosateEPSP, 5-enolpyruvyl-shikimate-3-phosphateshikimate, (3R,4S,5R)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acidannual bluegrass, Poa annua L.EPSP synthasegolf courseshikimate accumulationtarget site resistancetranslocationturfgrass
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 63 , Issue 3 , September 2015 , pp. 613 - 622
Copyright
Copyright © Weed Science Society of America 

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