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Rapid Identification and Molecular Characterization of Phytoene Desaturase Mutations in Fluridone-Resistant Hydrilla (Hydrilla verticillata)

Published online by Cambridge University Press:  20 January 2017

Lori K. Benoit  and
Donald H. Les
Lori K. Benoit*
Affiliation:
Department of Ecology and Evolutionary Biology, University of Connecticut, 75 N. Eagleville Rd, Unit 3043, Storrs, CT 06269
Donald H. Les
Affiliation:
Department of Ecology and Evolutionary Biology, University of Connecticut, 75 N. Eagleville Rd, Unit 3043, Storrs, CT 06269
*
Corresponding author's Email: lori.benoit@uconn.edu
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Abstract

Florida hydrilla populations have shown an alarming increase in resistance to fluridone, an herbicide used extensively for controlling invasive US hydrilla populations. A rapid PCR and sequencing method was developed to identify and screen hydrilla genomic DNA for three previously identified phytoene desaturase (pds) gene mutations that confer resistance to fluridone. Ninety hydrilla accessions were screened for fluridone resistant genotypes including 46 accessions from the US and 44 accessions from 15 other countries. In Florida, hydrilla from five of nine sites tested was heterozygous for wild-type and herbicide-resistant alleles. Additionally, a new resistant population was identified from Lake Seminole in Georgia, the first genetically confirmed strain of resistant hydrilla outside of Florida. All resistance-conferring mutations were located on the same homologous haplotype of US dioecious hydrilla. All other hydrilla samples tested possessed only wild type alleles, including monoecious strains that had been exposed to fluridone. Management implications are discussed.

Keywords

Fluridonehydrilla, Hydrilla verticillata (L.f.) Royle HYLLIAquatic weedherbicide resistanceinvasive speciesphytoene desaturase genepolymerase chain reaction PCRsequencingcloning
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 61 , Issue 1 , March 2013 , pp. 32 - 40
Copyright
Copyright © Weed Science Society of America 

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