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Rapid necrosis: a novel plant resistance mechanism to 2,4-D

Published online by Cambridge University Press:  05 November 2019

Andrew R. S. de Queiroz Open the ORCID record for Andrew R. S. de Queiroz [Opens in a new window] ,
Carla A. Delatorre Open the ORCID record for Carla A. Delatorre [Opens in a new window] ,
Felipe R. Lucio Open the ORCID record for Felipe R. Lucio [Opens in a new window] ,
Caio V. S. Rossi Open the ORCID record for Caio V. S. Rossi [Opens in a new window] ,
Luiz H. S. Zobiole Open the ORCID record for Luiz H. S. Zobiole [Opens in a new window]  and
Aldo Merotto Jr Open the ORCID record for Aldo Merotto Jr [Opens in a new window]
Andrew R. S. de Queiroz
Affiliation:
Graduate Student, Department of Crop Science, Federal University of Rio Grande do Sul–UFRGS, Porto Alegre, RS, Brazil
Carla A. Delatorre
Affiliation:
Professor, Department of Crop Science, Federal University of Rio Grande do Sul–UFRGS, Porto Alegre, RS, Brazil
Felipe R. Lucio
Affiliation:
Integrated Field Science, Dow AgroSciences Industrial LTDA, São Paulo, Brazil
Caio V. S. Rossi
Affiliation:
Integrated Field Science, Dow AgroSciences Industrial LTDA, São Paulo, Brazil
Luiz H. S. Zobiole
Affiliation:
Integrated Field Science, Dow AgroSciences Industrial LTDA, São Paulo, Brazil
Aldo Merotto Jr*
Affiliation:
Professor, Department of Crop Science, Federal University of Rio Grande do Sul–UFRGS, Porto Alegre, RS, Brazil
*
Author for correspondence: Aldo Merotto Jr, Department of Crop Science, Federal University of Rio Grande do Sul–UFRGS, 7712 Bento Goncalves Avenue, Porto Alegre, RS, Brazil91501-970. (Email: merotto@ufrgs.br)
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Abstract

Plants of Sumatran fleabane [Conyza sumatrensis (Retz.) E. Walker] were identified in a field with an unusual rapid leaf-injury herbicide symptoms after application of 2,4-D in mixture with glyphosate. The objectives of this study were to confirm the occurrence of resistance to 2,4-D herbicide and to characterize the occurrence of rapid necrosis as the mechanism associated with the herbicide resistance in C. sumatrensis. The studies performed were an initial screening, effect of 2,4-D alone and associated with glyphosate, cross- and multiple-resistance evaluation, effect of commercial formulation and analytical product, and rate of H2O2 evolution. The Marpr9-rn accession was identified with rapid necrosis symptoms and survival to 804 g ae ha−1 of 2,4-D. The resistance factor to the herbicide 2,4-D was 18.6 at 49 d after spraying. The analytical product 2,4-D and the commercial formulation resulted in similar symptoms of rapid necrosis. This symptom did not occur for the six other auxinic herbicides (dicamba, florpyrauxifen-benzyl, fluroxypyr, halauxifen-methyl, picloram, and triclopyr), indicating absence of cross-resistance. Multiple resistance to the herbicides paraquat, saflufenacil, and ammonium glufosinate was not identified in the Marpr9-rn population. However, survival following treatment with the herbicides glyphosate and chlorimuron-ethyl occurred. The evolution of H2O2 began at 15 min after application and was less pronounced in low light. These results indicate the first case of resistance to 2,4-D and occurrence of rapid necrosis in C. sumatrensis.

Keywords

Auxinherbicide resistancehypersensitive responserapid responsereactive oxygen speciesSumatran fleabanetall fleabane
Type
Research Article
Information
Weed Science , Volume 68 , Issue 1 , January 2020 , pp. 6 - 18
Copyright
© Weed Science Society of America, 2019

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Footnotes

Associate Editor: William Vencill, University of Georgia

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