Hostname: page-component-cd9895bd7-q99xh Total loading time: 0 Render date: 2024-12-26T20:38:37.440Z Has data issue: false hasContentIssue false
  • English
  • Français

Reduced Translocation Is Associated with Tolerance of Common Lambsquarters (Chenopodium album) to Glyphosate

Published online by Cambridge University Press:  20 January 2017

Melinda K. Yerka ,
Andrew T. Wiersma ,
R. Bradley Lindenmayer ,
Philip Westra ,
William G. Johnson ,
Natalia de Leon  and
David E. Stoltenberg
Melinda K. Yerka
Affiliation:
Department of Agronomy, University of Wisconsin-Madison, 1575 Linden Drive, Madison, WI 53705
Andrew T. Wiersma
Affiliation:
Bioagricultural Science and Pest Management, Colorado State University, Fort Collins, CO 80523-1170
R. Bradley Lindenmayer
Affiliation:
Bioagricultural Science and Pest Management, Colorado State University, Fort Collins, CO 80523-1170
Philip Westra
Affiliation:
Bioagricultural Science and Pest Management, Colorado State University, Fort Collins, CO 80523-1170
William G. Johnson
Affiliation:
Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47909
Natalia de Leon
Affiliation:
Department of Agronomy, University of Wisconsin-Madison, 1575 Linden Drive, Madison, WI 53705
David E. Stoltenberg*
Affiliation:
Department of Agronomy, University of Wisconsin-Madison, 1575 Linden Drive, Madison, WI 53705
*
Corresponding author's E-mail: destolte@wisc.edu
Get access Rights & Permissions [Opens in a new window]

Abstract

Common lambsquarters tolerance to glyphosate is problematic because of the species' widespread distribution, competitive ability with many crop species, the widespread use of glyphosate in agriculture, and the weed's potential to develop decreased sensitivity to multiple herbicide sites of action. The mechanism that confers common lambsquarters tolerance to glyphosate is not known. Therefore, we conducted experiments to determine the mechanism of tolerance to glyphosate in an accession of common lambsquarters from Indiana relative to a sensitive accession from Wisconsin. The ED50 (the effective dose that reduced shoot mass 50% relative to nontreated plants) value for the tolerant accession (1.6 kg ae ha−1 ± 0.4 standard error of the mean [SEM]) was eightfold greater than the ED50 for the sensitive accession (0.2 kg ae ha−1 ± 0.2 SEM) 28 d after treatment. The glyphosate target-site (EPSPS) DNA sequence at proline 106, shikimate accumulation as an estimate of EPSPS sensitivity, and EPSPS protein abundance did not differ between accessions. Absorption of 14C-glyphosate was slightly greater in the tolerant accession than it was in the sensitive accession at 48 and 72 h after treatment (HAT). However, the tolerant accession translocated a smaller percentage of absorbed 14C-glyphosate to the tissue above the treated leaf, which included the shoot apical meristem, at 24, 48, and 72 HAT (P ≤ 0.05, 0.01, and 0.10, respectively). These results suggest an important role of reduced translocation in conferring tolerance of common lambsquarters to glyphosate.

Keywords

EPSPS, 5-enolpyruvylshikimate-3-phosphate synthaseglyphosatecommon lambsquarters, Chenopodium album L. CHEALHerbicidemechanism of actionresistance
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 61 , Issue 3 , September 2013 , pp. 353 - 360
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

Amrhein, N., Deus, B., Gherke, P., and Steinruken, H. C. 1980. The site of inhibition of the shikimate pathway by glyphosate, II: interference of glyphosate with chorismate formation in vivo and in vitro . Plant Physiol. 66:830–834.Google Scholar
Bhargava, A., Shukla, S., and Ohri, D. 2006. Karyotypic studies on some cultivated and wild species of Chenopodium (Chenopodiaceae). Genet. Resour. Crop Evol. 53:1309–1320.Google Scholar
Bolt, M. W. and Mahoney, P. A. 1997. High-efficiency blotting of proteins of diverse sizes following sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Anal. Biochem. 247:185–192.Google Scholar
Bukun, B., Lindenmayer, R. B., Nissen, S. J., Westra, P. H., Shaner, D. L., and Brunk, G. 2010. Absorption and translocation of aminocyclopyrachlor and aminocyclopyrachlor-methyl ester in Canada thistle (Cirsium arvense). Weed Sci. 58:96–102.Google Scholar
Bukun, B., Gaines, T. A., Nissen, S. J., Westra, P. H., Brunk, G., Shaner, D. L., Sleugh, B. B., and Peterson, V. F. 2009. Aminopyralid and clopyralid absorption and translocation in Canada thistle (Cirsium arvense). Weed Sci. 57:10–15.Google Scholar
Conley, S. P., Stoltenberg, D. E., Boerboom, C. M., and Binning, L. K. 2003. Predicting soybean yield loss in giant foxtail (Setaria faberi) and common lambsquarters (Chenopodium album) communities. Weed Sci. 51:402–407.Google Scholar
Conn, J. S. and Werdin-Pfisterer, N. R. 2010. Variation in seed viability and dormancy of 17 weed species after 24.7 years of burial: the concept of buried seed safe sites. Weed Sci. 58:209–215.CrossRefGoogle Scholar
Conover, W. J. 1999. Some methods based on ranks. Pp. 269–427 in Wiley, B. W., ed. Practical Nonparametric Statistics. 3rd ed. New York J. Wiley.Google Scholar
Darmency, H. and Gasquez, J. 1990. Appearance and spread of triazine resistance in common lambsquarters (Chenopodium album). Weed Technol. 4:173–177.Google Scholar
Dinelli, G., Marotti, I., Bonetti, A., Minelli, M., Catizone, P., and Barnes, J. 2006. Physiological and molecular insight on the mechanisms of resistance to glyphosate in Conyza canadensis (L.) Cronq. biotypes. Amsterdam Elsevier. 12 p.Google Scholar
Feng, P.C.C., Tran, M., Chiu, T., Sammons, R. D., Heck, G. R., and Cajacob, C. A. 2004. Investigations into glyphosate-resistant horseweed (Conyza canadensis): retention, uptake, translocation and metabolism. Weed Sci. 52:498–505.Google Scholar
Gaines, T. A., Zhang, W. L., Wang, D., Bukun, B., Chisholm, S. T., Shaner, D. L., Nissen, S. J., Vencill, W. K., Sammons, R. D., Jiang, J., Preston, C., Leach, J. E., and Westra, P. H. 2010. Gene amplification confers glyphosate resistance in Amaranthus palmeri . Proc. Natl. Acad. Sci. U. S. A. 107:1029–1034.Google Scholar
Ge, X., D'avignon, D. A., Ackerman, J.J.H., and Sammons, R. D. 2010. Rapid vacuolar sequestration: the horseweed glyphosate resistance mechanism. Pest Manag. Sci. 66:345–348.Google Scholar
Hall, T. A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp. Ser. 41:95–98.Google Scholar
Harrison, S. K. 1990. Interference and seed production by common lambsquarters (Chenopodium album) in soybeans (Glycine max). Weed Sci. 38:113–118.Google Scholar
Heap, I. 2012. The International Survey of Herbicide Resistant Weeds http://www.weedscience.com/. Accessed February 3, 2012.Google Scholar
Herrmann, K. M. and Weaver, L. M. 1999. The shikimate pathway. Annu. Rev. Plant Physiol. Mol. Biol. 50:473–503.CrossRefGoogle ScholarPubMed
Hite, G. A., King, S. R., Hagood, E. S., and Holtzman, G. I. 2008. Differential response of a Virginia common lambsquarters (Chenopodium album) collection to glyphosate. Weed Sci. 56:203–209.Google Scholar
Holm, L. G., Plucknett, D. L., Pancho, J. V., and Herberger, J. P. 1977. Chenopodium album L. Pp. 84–91 in The World's Worst Weeds: Distribution and Biology. Honolulu, HI University Press of Hawaii, Honolulu.Google Scholar
Kaundun, S. S., Dale, R. P., Zelaya, I. A., Dinelli, G., Marotti, I., Mcindoe, E., and Cairns, A. 2011. A novel P106L mutation in EPSPS and an unknown mechanism(s) act additively to confer resistance to glyphosate in a South African Lolium rigidum population. J. Agric. Food Chem. 59:3227–3233.Google Scholar
Kniss, A. R. 2006. Tolerance of common lambsquarters (Chenopodium album L.) to glyphosate. Ph.D Dissertation. Laramie, WY University of Wyoming. 92 p.Google Scholar
Kniss, A. R., Vassios, J. D., Nissen, S. J., and Ritz, C. 2011. Nonlinear regression analysis of herbicide absorption studies. Weed Sci. 59:601–610.Google Scholar
Kniss, A. R., Miller, S. D., Westra, P. H., and Wilson, R. G. 2007. Glyphosate susceptibility in common lambsquarters (Chenopodium album) is influenced by parental exposure. Weed Sci. 55:572–577.Google Scholar
Koger, C. H. and Reddy, K. N. 2005. Role of absorption and translocation in the mechanism of glyphosate resistance in horseweed (Conyza canadensis). Weed Sci. 53:84–89.Google Scholar
Kruger, G. R., Johnson, W. G., Weller, S. C., Owen, M.D.K., Shaw, D. R., Wilcut, J. W., Jordan, D. L., Wilson, R. G., Bernards, M. L., and Young, B. G. 2009. U.S. grower views on problematic weeds and changes in weed pressure in glyphosate-resistant corn, cotton, and soybean cropping systems. Weed Technol. 23:162–166.Google Scholar
Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685.CrossRefGoogle ScholarPubMed
Michitte, P., De Prado, R., Espinoza, N., Ruiz-Santaella, J. P., and Gauvrit, C. 2007. Mechanisms of resistance to glyphosate in a ryegrass (Lolium multiflorum) biotype from Chile. Weed Sci. 55:435–440.Google Scholar
Moechnig, M. J., Stoltenberg, D. E., Boerboom, C. M., and Binning, L. K. 2003. Empirical corn-yield loss estimation from common lambsquarters (Chenopodium album) and giant foxtail (Setaria faberi) in mixed communities. Weed Sci. 51:386–393.Google Scholar
Mueller, T. C., Massey, J. H., Hayes, R. M., Main, C. L., Stewart, J., and C. N. 2003. Shikimate accumulates in both glyphosate-sensitive and glyphosate-resistant horseweed (Conyza canadensis L. Cronq.). J. Agric. Food Chem. 51:680–684.CrossRefGoogle ScholarPubMed
Norsworthy, J. K., Riar, D. S., Jha, P., and Scott, R. C. 2011. Confirmation, control, and physiology of glyphosate-resistant giant ragweed (Ambrosia trifida) in Arkansas. Weed Technol. 25:430–435.CrossRefGoogle Scholar
Perez-Jones, A. K., Park, K. W., Polge, N., Colquhoun, J., and Mallory-Smith, C. 2007. Investigating the mechanisms of glyphosate resistance in Lolium multiflorum . Planta 226:395–404.Google Scholar
Powles, S. B. and Preston, C. 2006. Evolved glyphosate resistance in plants: biochemical and genetic basis of resistance. Weed Technol. 20:282–289.Google Scholar
Powles, S. B. and Yu, Q. 2010. Evolution in action: plants resistant to herbicides. Annu. Rev. Plant Biol. 61:317–347.Google Scholar
Preston, C., Shaner, D. L., Westra, P. H., Culpepper, A. S., Grey, T. L., Vencill, W. K., and Wakelin, A. M. 2006. Can a shikimate assay be used to determine the genotype of glyphosate resistant plants for population genetic studies? Pp. 511–514 in Preston, C., Watts, J. H., and Crossman, N. D., eds. Managing Weeds in a Changing Climate: Papers and Proceedings of the 15th Australian Weeds Conference. Adelaide, South Australia Council of Australasian Weed Societies.Google Scholar
Riar, D. S., Norsworthy, J. K., Johnson, D. B., Scott, R. C., and Bagavathiannan, M. 2011. Glyphosate resistance in a johnsongrass (Sorghum halepense) biotype from Arkansas. Weed Sci. 59:299–304.Google Scholar
Ritz, C. 2010. Toward a unified approach to dose–response modeling in ecotoxicology. Environ. Toxicol. Chem. 29:220–229.Google Scholar
Rogers, S. O. and Bendich, A. J. 1985. Extraction of DNA from milligram amounts of fresh, herbarium, and mummified plant tissues. Plant Mol. Biol. 5:69–76.Google Scholar
Ryerse, J. S., Downer, R. A., Sammons, R. D., and Feng, P. C. C. 2004. Effect of glyphosate spray droplets on leaf cytology in velvetleaf (Abutilon theophrasti). Weed Sci. 52:302–309.Google Scholar
Saini, H. S., Bassi, P. K., and Spencer, M. S. 1986. Use of ethylene and nitrate to break seed dormancy of common lambsquarters (Chenopodium album). Weed Sci. 34:502–506.Google Scholar
Shaner, D. L. 2009. Role of translocation as a mechanism of resistance to glyphosate. Weed Sci. 57:118–123.CrossRefGoogle Scholar
Shaner, D. L., Lindenmeyer, R. B., and Ostlie, M. H. 2012. What have the mechanisms of resistance to glyphosate taught us? Pest. Manag. Sci. 68:3–9.CrossRefGoogle ScholarPubMed
Shaner, D. L., Nadler-Hassar, T., Henry, W. B., and Koger, C. H. 2005. A rapid in vivo shikimate accumulation assay with excised leaf discs. Weed Sci. 53:769–774.Google Scholar
Sivesind, E. C., Gaska, J. M., Jeschke, M. R., Boerboom, C. M., and Stoltenberg, D. E. 2011. Common lambsquarters response to glyphosate across environments. Weed Technol. 25:44–50.Google Scholar
Steel, R.G.D. and Torrie, J. H. 1980. Principles and procedures of statistics: A biometrical approach. 2nd ed. New York McGraw-Hill. 633 p.Google Scholar
Stoltenberg, D. E. and Wiederholt, R. J. 1995. Giant foxtail (Setaria faberi) resistance to aryloxyphenoxypropionate and cyclohexanedione herbicides. Weed Sci. 43:527–535.Google Scholar
Stoltenberg, D., Yerka, M., Glettner, C., Stute, J., and Trower, T. 2012. Giant ragweed resistance to glyphosate in Wisconsin. Wis. Crop Manager. 19:43–44 http://ipcm.wisc.edu/wcm/ Accessed June 21, 2012.Google Scholar
Vila-Aiub, M. M., Balbi, M. C., Gundel, P. E., Ghersa, C. M., and Powles, S. B. 2007. Evolution of glyphosate-resistant johnsongrass (Sorghum halepense) in glyphosate-resistant soybean. Weed Sci. 55:566–571.Google Scholar
Wakelin, A. M. and Preston, C. 2006. Inheritance of glyphosate resistance in several populations of rigid ryegrass (Lolium rigidum) from Australia. Weed Sci. 54:212–219.Google Scholar
Wakelin, A. M., Lorraine-Colwill, D. F., and Preston, C. 2004. Glyphosate resistance in four different populations of Lolium rigidum is associated with reduced translocation of glyphosate to meristematic zones. Weed. Res. 44:453–459.Google Scholar
[WSSA] Weed Science Society of America. 1998. Herbicide resistance and herbicide tolerance defined. Weed Technol. 12:789.Google Scholar
Westhoven, A. M., Kruger, G. R., Gerber, C. K., Stachler, J. M., Loux, M. M., and Johnson, W. G. 2008. Characterization of selected common lambsquarters (Chenopodium album) biotypes with tolerance to glyphosate. Weed Sci. 56:685–691.Google Scholar
Yerka, M. K. 2011. Glyphosate tolerance and gene flow in common lambsquarters (Chenopodium album L.) Ph.D dissertation. Madison, WI University of Wisconsin-Madison. 119 p.Google Scholar
Yerka, M. K., de Leon, N., and Stoltenberg, D. E. 2012. Pollen-mediated gene flow in common lambsquarters (Chenopodium album). Weed Sci. 60:600–606.CrossRefGoogle Scholar
Yu, Q., Abdallah, I., Han, H. P., Owen, M., and Powles, S. B. 2009. Distinct nontarget-site mechanisms endow resistance to glyphosate, ACCase and ALS-inhibiting herbicides in multiple herbicide-resistant Lolium rigidum populations. Planta 230:713–723.Google Scholar