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A Survey of Asymbiotic Nitrogen Fixation in the Rhizosphere of Weeds

Published online by Cambridge University Press:  12 June 2017

A. R. Conklin Jr.
Affiliation:
Agric. Dep., Wilmington College, Wilmington, OH 45177
P. K. Biswas
Affiliation:
Agric. Dep., Tuskegee Inst., Tuskegee, AL 36083

Abstract

The occurrence of asymbiotic nitrogen fixing bacteria in the rhizosphere of twenty weed species has been investigated. Using the roll tube method root-rhizosphere soil samples were diluted and added to test tubes containing low N media. Tubes were flushed with nitrogen, stoppered, and rolled to form a thin film of media on the inner wall. After 7 days incubation the nitrogen fixation rates were estimated using the acetylene reduction technique. Rates ranged from 38 to 783 nmoles acetylene reduced per hour. Of twenty species tested, three showed high rhizosphere populations of asymbiotic nitrogen fixing bacteria.

Type
Research Article
Copyright
Copyright © 1978 by the Weed Science Society of America 

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