Hostname: page-component-cd9895bd7-q99xh Total loading time: 0 Render date: 2024-12-27T10:23:05.507Z Has data issue: false hasContentIssue false
  • English
  • Français

Target Enzyme-Based Resistance to Clethodim in Lolium rigidum Populations in Australia

Published online by Cambridge University Press:  20 January 2017

Rupinder Kaur Saini ,
Jenna Malone ,
Christopher Preston  and
Gurjeet Gill
Rupinder Kaur Saini*
Affiliation:
School of Agriculture, Food and Wine, University of Adelaide, PMB 1, Glen Osmond, South Australia, 5064
Jenna Malone
Affiliation:
School of Agriculture, Food and Wine, University of Adelaide, PMB 1, Glen Osmond, South Australia, 5064
Christopher Preston
Affiliation:
School of Agriculture, Food and Wine, University of Adelaide, PMB 1, Glen Osmond, South Australia, 5064
Gurjeet Gill
Affiliation:
School of Agriculture, Food and Wine, University of Adelaide, PMB 1, Glen Osmond, South Australia, 5064
*
Corresponding author's E-mail: rupinder.saini@adelaide.edu.au
Get access Rights & Permissions [Opens in a new window]

Abstract

Clethodim resistance was identified in 12 rigid ryegrass populations from winter cropping regions in four different states of Australia. Clethodim had failed to provide effective control of these populations in the field and resistance was suspected. Dose–response experiments confirmed resistance to clethodim and butroxydim in all populations. During 2012, the LD50 of resistant populations ranged from 10.2 to 89.3 g ha−1, making them 3 to 34–fold more resistant to clethodim than the susceptible population. Similarly, GR50 of resistant population varied from 8 to 37.1 g ha−1, which is 3 to 13.9–fold higher than the susceptible population. In 2013, clethodim-resistant populations were 7.8 to 35.3–fold more resistant to clethodim than the susceptible population. The higher resistance factor in 2013, especially in moderately resistant populations, could have been associated with lower ambient temperatures during the winter of 2013. These resistant populations had also evolved cross-resistance to butroxydim. The resistant populations required 1.3 to 6.6–fold higher butroxydim dose to achieve 50% mortality and 3 to 27–fold more butroxydim for 50% biomass reduction compared to the standard susceptible population. Sequencing of the target-site ACCase gene identified five known ACCase substitutions (isoleucine-1781-leucine, isoleucine-2041-asparagine, aspartate-2078-glycine, and cysteine-2088-arginine, and glycine-2096-alanine) in these populations. In nine populations, multiple ACCase mutations were present in different individuals. Furthermore, two alleles with different mutations were present in a single plant of rigid ryegrass in two populations.

Keywords

Clethodimrigid ryegrassLolium rigidum Gaudin.ACCase genebutroxydimclethodimresistance mechanismtarget-site mutation
Type
Weed Management
Information
Weed Science , Volume 63 , Issue 4 , December 2015 , pp. 946 - 953
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

Associate Editor for this paper: William Vencill, University of Georgia.

References

Literature Cited

Ahmad-Hamdani, MS, Owen, MJ, Yu, Q, Powles, SB (2012) ACCase-inhibiting herbicide-resistant Avena spp. populations from the western Australian grain belt. Weed Technol 26:130–136.Google Scholar
Beckie, HJ, Tardif, HJ (2012) Herbicide cross resistance in weeds. Crop Prot 35:15–28.Google Scholar
Boutsalis, P, Gill, GS, Preston, C (2012) Incidence of herbicide resistance in rigid ryegrass (Lolium rigidum) across southeastern Australia. Weed Technol 26:391–398.Google Scholar
Bradley, KW, Wu, J, Hatzios, KK, Hagood, ES. Jr. (2009) The mechanism of resistance to aryloxyphenoxypropionate and cyclohexanedione herbicides in a johnsongrass biotype. Weed Sci 49:477–484.Google Scholar
Broster, J, Koetz, E, Wu, H (2011) Herbicide resistance levels in annual ryegrass (Lolium rigidum Gaud.) in southern New South Wales. Plant Prot Q 26:22–28.Google Scholar
Burke, IC, Price, AJ, Wilcut, JW, Jordan, DL, Culpepper, S, Tredaway-Ducar, J (2004) Annual grass control in peanut (Arachis hypogaea) with clethodim and imazapic. Weed Technol 18:88–92.Google Scholar
Charmet, G, Balfourier, F, Chatard, V (1996) Taxonomic relationships and interspecific hybridization in the genus Lolium (grasses). Genet Resour Crop Evol 43:319–327.Google Scholar
Christopher, JT, Powles, SB, Holtum, JAM (1992) Resistance to acetolactate synthase-inhibiting herbicides in annual ryegrass (Lolium rigidum) involves at least two mechanisms. Plant Physiol 100:1909–1913.Google Scholar
Cruz-Hipolito, H, Domínguez-Valenzuela, JA, Osuna, MD, De Prado, R (2012) Resistance mechanism to acetyl coenzyme A carboxylase inhibiting herbicides in Phalaris paradoxa collected in Mexican wheat fields. Plant Soil 355:121–130.Google Scholar
Cruz-Hipolito, H, Osuna, MD, Domínguez-Valenzuela, JA, Espinoza, N, De Prado, R (2011) Mechanism of resistance to ACCase-inhibiting herbicides in wild oat (Avena fatua) from Latin America. J Agric Food Chem 59:7261–7267.Google Scholar
Délye, C (2005) Weed resistance to acetyl coenzyme A carboxylase inhibitors: an update. Weed Sci 53:728–746.Google Scholar
Devine, MD (1997) Mechanisms of resistance to acetyl-coenzyme A carboxylase inhibitors: a review. Pestic Sci 51:259–264.Google Scholar
Devine, MD, Shimabukuro, RH (1994) Resistance to acetyl coenzyme A carboxylase inhibiting herbicides. Pages 141–169 in Powles, S, Holtum, J, eds. Herbicide Resistance in Plants: Biology and Biochemistry. Boca Raton, FL Lewis Publishers.Google Scholar
Gill, GS (1996) Ecology of annual ryegrass. Plant Prot Q 11:195–198.Google Scholar
Gramshaw, D (1972) Germination of annual ryegrass seeds (Lolium rigidum Gaud.) as influenced by temperature, light, storage environment, and age. Aust J Agric Res 23:779–787.Google Scholar
Gronwald, JW, Eberlein, CV, Betts, KJ, Baerg, RJ, Ehlke, NJ, Wyse, DL (1992) Mechanism of diclofop resistance in an Italian ryegrass (Lolium multiflorum Lam.) biotype. Pest Biochem Physiol 44:126–139.Google Scholar
Harwood, JL (1988) Fatty acid metabolism. Annu Rev Plant Physiol 39:101–138.Google Scholar
Heap, I (2015) The international survey of herbicide-resistant weeds. http://www.weedscience.org/In.asp. Accessed April 2, 2015.Google Scholar
Hochberg, O, Sibony, M, Rubin, B (2009) The response of ACCase-resistant Phalaris paradoxa populations involves two different target site mutations. Weed Res 49:37–46.Google Scholar
Hofer, U, Muehlebach, M, Hole, S, Zoschke, A (2006) Pinoxaden-for broad spectrum grass weed management in cereal crops. J Plant Dis Prot 113:989–995.Google Scholar
Holtum, JAM, Matthews, JM, Häusler, RE, Liljegren, DR, Powles, SB (1991) Cross-resistance to herbicides in annual ryegrass (Lolium rigidum): III. On the mechanism of resistance to diclofop-methyl. Plant Physiol 97:1026–1034.Google Scholar
Jang, S, Marjanovic, J, Gornicki, P (2013) Resistance to herbicides caused by single amino acid mutations in acetyl-CoA carboxylase in resistant populations of grassy weeds. New Phytol 197:1110–1116.Google Scholar
Johnson, BC, Young, BG, Matthews, JL (2002) Effect of postemergence application rate and timing of mesotrione on corn (Zea mays) response and weed control. Weed Technol 16:414–420.Google Scholar
Jones, RE, Vere, DT, Alemseged, Y, Medd, RW (2005) Estimating the economic cost of weeds in Australian annual winter crops. Agric Econ 32:253–265.Google Scholar
Kells, JJ, Meggitt, WF, Penner, D (1984) Absorption, translocation, and activity of fluazifop-butyl as influenced by plant growth stage and environment. Weed Sci 32:143–149.Google Scholar
Liu, W, Harrison, DK, Chalupska, D, Gornicki, P, O'Donnell, CC, Adkins, SW, Haselkorn, R, Williams, RR (2007) Single-site mutations in the carboxyltransferase domain of plastid acetyl-CoA carboxylase confer resistance to grass-specific herbicides. Proc Natl Acad Sci 104:3627–3632.Google Scholar
Lorraine-Colwill, DF, Powles, SB, Hawkes, TR, Preston, C (2001) Inheritance of evolved glyphosate resistance in Lolium rigidum (Gaud.). Theor Appl Genet 102:545–550.Google Scholar
Malone, JM, Boutsalis, P, Baker, J, Preston, C (2014) Distribution of herbicide-resistant acetyl-coenzyme A carboxylase alleles in Lolium rigidum across grain cropping areas of south Australia. Weed Res 54:78–86.Google Scholar
Martins, BAB, Sánchez-Olguín, E, Perez-Jones, A, Hulting, AG, Mallory-Smith, C (2014) Alleles contributing to ACCase-resistance in an Italian ryegrass (Lolium perenne ssp. multiflorum) population from Oregon. Weed Sci 62:468–473.Google Scholar
McAlister, FM, Holtum, JAM, Powles, SB (1995) Dintroaniline herbicide resistance in rigid ryegrass (Lolium rigidum). Weed Sci 43:55–62.Google Scholar
Muehlebach, M, Boeger, M, Cederbaum, F, Cornes, D, Friedmann, AA, Glock, J, Niderman, T, Stoller, A, Wagner, T (2009) Aryldiones incorporating a [1,4,5] oxadiazepane ring. Part I: discovery of the novel cereal herbicide pinoxaden. Bioorg Medical Chem 17:4241–4256.Google Scholar
Neve, P, Powles, S (2005) Recurrent selection with reduced herbicide rates results in the rapid evolution of herbicide resistance in Lolium rigidum . Theor Appl Genet 110:1154–1166.Google Scholar
Owen, MJ, Powles, SB (2009) Distribution and frequency of herbicide-resistant wild oat (Avena spp.) across the western Australian grain belt. Crop Pasture Sci 60:25–31.Google Scholar
Powles, SB, Lorraine-Colwill, DF, Dellow, JJ, Preston, C (1998) Evolved resistance to glyphosate in rigid ryegrass (Lolium rigidum) in Australia. Weed Sci 46:604–607.Google Scholar
Powles, SB, Yu, Q (2010) Evolution in action: plants resistant to herbicides. Annu Rev Plant Biol 61:317–347.Google Scholar
Preston, C, Roush, RT, Powles, SB (1999) Herbicide resistance in weeds of southern Australia: why are we the worst in the world. Pages 454–459 in Proceedings of the 12th Australian Weeds Conference. Devonport, Australia Tasmanian Weed Society.Google Scholar
Preston, C, Tardif, FJ, Christopher, JT, Powles, SB (1996) Multiple resistance to dissimilar herbicide chemistries in a biotype of Lolium rigidum due to enhanced activity of several herbicide degrading enzymes. Pest Biochem Physiol 54:123–134.Google Scholar
Rerkasem, K, Stern, WR, Goodchild, NA (1980) Associated growth of wheat and annual ryegrass. 1. effect of varying total density and proportion in mixtures of wheat and annual ryegrass. Aust J Agric Res 31:549–658.Google Scholar
Tang, W, Zhou, F, Chen, J, Zhou, X (2014) Resistance to ACCase-inhibiting herbicides in an Asia minor bluegrass (Polypogon fugax) population in China. Pest Biochem Physiol 108:16–20.Google Scholar
Wakelin, A, Preston, C (2006) A target-site mutation is present in a glyphosate-resistant Lolium rigidum population. Weed Res 46:432–440.Google Scholar
Yu, Q, Ahmad-Hamdani, M, Han, H, Christoffers, M, Powles, S (2013a) Herbicide resistance-endowing ACCase gene mutations in hexaploid wild oat (Avena fatua): insights into resistance evolution in a hexaploid species. Heredity 110:220–231.Google Scholar
Yu, Q, Collavo, A, Zheng, M-Q, Owen, M, Sattin, M, Powles, SB (2007) Diversity of acetyl-coenzyme A carboxylase mutations in resistant Lolium populations: evaluation using clethodim. Plant Physiol 145:547–558.Google Scholar
Yu, Q, Han, H, Cawthray, GR, Wang, SF, Powles, SB (2013b) Enhanced rates of herbicide metabolism in low herbicide-dose selected resistant Lolium rigidum . Plant Cell Environ 36:818–827.Google Scholar
Zhang, XQ, Powles, SB (2006) Six amino acid substitutions in the carboxyl-transferase domain of the plastidic acetyl-CoA carboxylase gene are linked with resistance to herbicides in a Lolium rigidum population. New Phytol 172:636–645.Google Scholar