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Target-Site Point Mutation Conferring Resistance to Trifluralin in Rigid Ryegrass (Lolium rigidum)

Published online by Cambridge University Press:  30 October 2017

Benjamin Fleet ,
Jenna Malone ,
Christopher Preston  and
Gurjeet Gill
Benjamin Fleet*
Affiliation:
Post-graduate Student, Post-doctoral fellow, Associate Professor, and Associate Professor, School of Agriculture, Food and Wine, University of Adelaide, PMB 1, Glen Osmond, South Australia, 5064
Jenna Malone
Affiliation:
Post-graduate Student, Post-doctoral fellow, Associate Professor, and Associate Professor, School of Agriculture, Food and Wine, University of Adelaide, PMB 1, Glen Osmond, South Australia, 5064
Christopher Preston
Affiliation:
Post-graduate Student, Post-doctoral fellow, Associate Professor, and Associate Professor, School of Agriculture, Food and Wine, University of Adelaide, PMB 1, Glen Osmond, South Australia, 5064
Gurjeet Gill
Affiliation:
Post-graduate Student, Post-doctoral fellow, Associate Professor, and Associate Professor, School of Agriculture, Food and Wine, University of Adelaide, PMB 1, Glen Osmond, South Australia, 5064
*
Corresponding author’s E-mail: benjamin.fleet@adelaide.edu.au
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Abstract

Populations of rigid ryegrass suspected of resistance to trifluralin due to control failures exhibited varying levels of susceptibility to trifluralin, with 15 out of 17 populations deemed resistant (>20% plant survival). Detailed dose–response studies were conducted on one highly resistant field-evolved population (SLR74), one known multiply resistant population (SLR31), and one susceptible population (VLR1). On the basis of the dose required to kill 50% of treated plants (LD50), SLR74 had 15-fold greater resistance than VLR1, whereas, the multiply resistant SLR31 had 10-fold greater resistance than VLR1. Similarly, on the basis of dose required to reduce shoot biomass by 50% (GR50), SLR74 had 17-fold greater resistance than VLR1, and SLR31 had 8-fold greater resistance than VLR1. Sequencing of the α-tubulin gene from resistant plants of different populations confirmed the presence of a previously known goosegrass mutation causing an amino acid substitution at position 239 from threonine to isoleucine in resistant population SLR74. This mutation was also found in 4 out of 5 individuals in another highly resistant population TR2 and in 3 out of 5 individuals of TR4. An amino acid substitution from valine to phenylalanine at position 202 was also observed in TR4 (3 out of 5 plants) and TR2 (1 out of 5 plants). There was no target-site mutation identified in SLR31. This study documents the first known case of field-evolved target-site resistance to dinitroaniline herbicides in a population of rigid ryegrass.

Keywords

Trifluralinrigid ryegrass Lolium rigidum Gaudingoosegrass Eleusine indica (L.) Gaertn.α-Tubulin mutationdinitroaniline herbicidesherbicide resistancemechanismtarget-site
Type
Weed Management
Information
Weed Science , Volume 66 , Issue 2 , March 2018 , pp. 246 - 253
Copyright
© Weed Science Society of America, 2017 

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Footnotes

Associate Editor for This Paper: Franck E. Dayan, Colorado State University.

References

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