Hostname: page-component-78c5997874-ndw9j Total loading time: 0 Render date: 2024-11-10T10:30:45.071Z Has data issue: false hasContentIssue false
  • English
  • Français

Target-Site Resistance Mechanisms to Tribenuron-methyl and Cross-resistance Patterns to ALS-inhibiting Herbicides of Catchweed Bedstraw (Galium aparine) with Different ALS Mutations

Published online by Cambridge University Press:  18 December 2018

Wei Deng ,
Yingjie Di ,
Jingxuan Cai ,
Yueyang Chen  and
Shuzhong Yuan
Wei Deng
Affiliation:
Doctor, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China
Yingjie Di
Affiliation:
Graduate Student, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China
Jingxuan Cai
Affiliation:
Graduate Student, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China
Yueyang Chen
Affiliation:
Graduate Student, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China
Shuzhong Yuan*
Affiliation:
Associate Professor, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, China
*
Author for correspondence: Shuzhong Yuan, College of Horticulture and Plant Protection, Yangzhou University, No. 88 of Da Xue Nan Road, Hanjiang District, Yangzhou, China. (Email: yuansz10201@163.com)
Get access Rights & Permissions [Opens in a new window]

Abstract

Catchweed bedstraw (Galium aparine L.) is a problematic dicot weed that occurs in major winter wheat (Triticum aestivum L.) fields in China. Tribenuron-methyl has been widely used to control broadleaf weeds since 1988 in China. However, overuse has led to the resistance evolution of G. aparine to tribenuron-methyl. In this study, 20 G. aparine populations collected from Shandong and Henan provinces were used to determine tribenuron-methyl resistance and target-site resistance mechanisms. In dose–response experiments, 12 G. aparine populations showed different resistance levels (2.92 to 842.41-fold) to tribenuron-methyl compared with the susceptible population. Five different acetolactate synthase (ALS) mutations (Pro-197-Leu, Pro-197-Ser, Pro-197-His, Asp-376-Glu, and Trp-574-Leu) were detected in different resistant populations. Individuals heterozygous for Pro-197-Ser and Trp-574-Leu mutations were also observed in a resistant population (HN6). In addition, pHB4 (Pro-197-Ser), pHB7 (Pro-197-His), pHB8 (Pro-197-Leu), pHB5 (Asp-376-Glu), and pHB3 (Trp-574-Leu) subpopulations individually homozygous for specific ALS mutations were generated to evaluate the cross-resistance to ALS-inhibiting herbicides. The pHB4, pHB7, pHB8, pHB5, and pHB3 subpopulations all were resistant to sulfonylurea, pyrazosulfuron-ethyl, triazolopyrimidine, flumetsulam, sulfonylamino-carbonyl-triazolinone, flucarbazone-sodium, pyrimidinyl thiobenzoate, pyribenzoxim, and the imidazolinone imazethapyr. These results indicated the diversity of the resistance-conferring ALS mutations in G. aparine, and all these mutations resulted in broad cross-resistance to five kinds of ALS-inhibiting herbicides.

Keywords

Franck E. Dayan, Colorado State UniversityALSmutationresistancetarget-sitetribenuron-methyl
Type
Research Article
Information
Weed Science , Volume 67 , Issue 2 , March 2019 , pp. 183 - 188
Copyright
© Weed Science Society of America, 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Chen, J, Huang, Z, Zhang, C, Huang, H, Wei, S, Chen, J, Wang, X (2015) Molecular basis of resistance to imazethapyr in redroot pigweed (Amaranthus retroflexus L.) populations from China. Pestic Biochem Physiol 124:43–47 Google Scholar
Corbett, CA, Tardif, FJ (2006) Detection of resistance to acetolactate synthase inhibitors in weeds with emphasis on DNA-based techniques: a review. Pest Manag Sci 7:584–597 Google Scholar
Cui, HL, Li, XJ, Wang, GQ, Wang, JP, Wei, SH, Cao, HY (2012) Acetolactate synthase proline (197) mutations confer tribenuron-methyl resistance in Capsella bursa-pastoris populations from China. Pestic Biochem Physiol 102:229–232 Google Scholar
Cui, HL, Wang, CY, Xu, LL, Li, XJ (2016) Rapid molecular detection of the resistance of Galium aparine var. tenerum to AHAS inhibitors. J Plant Protect 43:1049–1054. Chinese Google Scholar
Cui, HL, Zhang, CX, Wei, SH, Zhang, HJ, Li, XJ, Zhang, YQ, Wang, GQ (2011) Acetolactate synthase gene proline (197) mutations confer tribenuron-methyl resistance in flixweed (Descurainia sophia) populations from China. Weed Sci 59:376–379 Google Scholar
Deng, W, Cao, Y, Yang, Q, Liu, M, Mei, Y, Zheng, M (2014) Different cross-resistance patterns to AHAS herbicides of two tribenuron-methyl resistant flixweed (Descurainia sophia L.) biotypes in China. Pestic Biochem Physiol 112:26–32 Google Scholar
Deng, W, Liu, MJ, Yang, Q, Mei, Y, Li, XF, Zheng, MQ (2015) Tribenuron-methyl resistance and mutation diversity of Pro197 in flixweed (Descurainia Sophia L.) accessions from China. Pestic Biochem Physiol 117:68–74 Google Scholar
Deng, W, Yang, Q, Jiao, HT, Zhang, YZ, Li, XF, Zheng, MQ (2016) Cross-resistance pattern to four AHAS-inhibiting herbicides of tribenuron-methyl-resistant flixweed (Descurainia sophia) conferred by Asp-376-Glu mutation in AHAS. J Integr Agr 15:2563–2570 Google Scholar
Deng, W, Yang, Q, Zhang, YZ, Jiao, HT, Mei, Y, Li, XF, Zheng, MQ (2017) Cross-resistance patterns to acetolactate synthase (ALS)-inhibiting herbicides of flixweed (Descurainia sophia L.) conferred by different combinations of ALS isozymes with a Pro-197-Thr mutation or a novel Trp-574-Leu mutation. Pestic Biochem Physiol 136:41–45 Google Scholar
Duggleby, RG, McCourt, JA, Guddat, LW (2008) Structure and mechanism of inhibition of plant acetohydroxyacid synthase. Plant Physiol Biochem 46:309–324 Google Scholar
Gao, XJ, Zhang, YM, Wang, QD, Guo, MY, Wang, HL, Ma, YH, Gao, XY, Liu, Y, Li, W (2016) Detection of bedstraw resistance to tribenuron-methyl in Henan province. Plant Protection 42:181–186. Chinese Google Scholar
Han, H, Yu, Q, Purba, E, Li, M, Walsh, MJ, Friesen, S, Powles, SB (2012) A novel amino acid substitution Ala-122-Tyr in ALS confers high-level and broad resistance across ALS-inhibiting herbicides. Pest Manag Sci 68:1164–1170 Google Scholar
Hatami, ZM, Gherekhloo, J, Rojano-Delgado, AM, Osuna, MD, Alcántara, R, Fernández, P, Sadeghipour, HR, Prado, RD (2016) Multiple mechanisms increase levels of resistance in Rapistrum rugosum to ALS herbicides. Front Plant Sci 7:169–181 Google Scholar
Heap, I (2018) The International Survey of Herbicide Resistant Weeds. www.weedscience.com. Accessed: June 16, 2018 Google Scholar
Huang, Z, Chen, J, Zhang, C, Huang, H, Wei, S, Chen, J, Wang, X (2016) Target-site basis for resistance to imazethapyr in redroot amaranth (Amaranthus retroflexus L.). Pestic Biochem Physiol 128:10–15 Google Scholar
Li, D, Li, X, Yu, H, Wang, J, Cui, H (2017) Cross-resistance of eclipta (Eclipta prostrata) in China to ALS inhibitors due to a Pro-197-Ser point mutation. Weed Sci 65:547–556 Google Scholar
Liu, W, Bi, Y, Li, L, Yuan, G, Wang, J (2013) Molecular basis of resistance to tribenuron in water starwort (Myosoton aquaticum) populations from China. Weed Sci 61:390–395 Google Scholar
Liu, W, Wu, C, Guo, W, Du, L, Yuan, G, Wang, J (2015) Resistance mechanisms to an acetolactate synthase (ALS) inhibitor in water starwort (Myosoton aquaticum) populations from China. Weed Sci 63:770–780 Google Scholar
Mazur, BJ, Falco, SC (1989) The development of herbicide resistant crops. Annu Rev Plant Biol 40:441–470 Google Scholar
McNaughton, KE, Letarte, J, Lee, EA, Tardif, FJ (2005) Mutations in ALS confer herbicide resistance in redroot pigweed (Amaranthus retroflexus) and Powell amaranth (Amaranthus powellii). Weed Sci 53:17–22 Google Scholar
Pandolfo, CE, Presotto, A, Moreno, F, Dossou, I, Migasso, JP, Sakima, E, Cantamutto, M (2016) Broad resistance to acetohydroxyacid-synthase-inhibiting herbicides in feral radish (Raphanus sativus L.) populations from Argentina. Pest Manag Sci 72:354–361 Google Scholar
Peng, XG, Wang, JX, Duan, M, Yang, JH (2008) The resistance to tribenuron-methyl in Galium aparine in winter wheat fields in northern China. Acta Phytophylacica Sin 35:458–462. Chinese Google Scholar
Rey-Caballero, J, Menéndez, J, Osuna, MD, Salas, M, Torra, J (2017) Target-site and non-target-site resistance mechanisms to ALS inhibiting herbicides in Papaver rhoeas . Pestic Biochem Physiol 138:57–65 Google Scholar
Seefeldt, SS, Jensen, JE, Fuerst, EP (1995) Log-logistic analysis of herbicide dose–response relationships. Weed Technol 9:218–227 Google Scholar
Singh, BK, Shaner, DL (1995) Biosynthesis of branched chain amino acids: from test tube to field. Plant Cell 7:935–944 Google Scholar
Sun, J, Wang, JX, Zhang, HJ, Liu, JL, Bian, SN (2011) Study on mutations in ALS for resistance to tribenuron-methyl in Galium aparine L. Agr Sci China 10:86–91 Google Scholar
Tranel, PJ, Wright, TR, Heap, IM (2018) Mutations in Herbicide-Resistant Weeds to ALS Inhibitors. www.weedscience.com. Accessed: June 16, 2018 Google Scholar
Vigueira, CC, Olsen, KM, Caicedo, AL (2013) The red queen in the corn: agricultural weeds as models of rapid adaptive evolution. Heredity 110:303–311 Google Scholar
Wang, HC, Xiao, WZ, Lou, YL, Sun, YC, Xu, XL (2017) Differences in sensitivity to several herbicides among different geographical populations of Galium aparine in Jiangsu province. J Weed Sci 35:16–21. Chinese Google Scholar
Warwick, SI, Sauder, C, Beckie, HJ (2005) Resistance in Canadian biotypes of wild mustard (Sinapis arvensis) to acetolactate synthase inhibiting herbicides. Weed Sci 53:631–639 Google Scholar
Warwick, SI, Xu, R, Sauder, C, Beckie, HJ (2008) Acetolactate synthase target-site mutations and single nucleotide polymorphism genotyping in ALS-resistant kochia (Kochia scoparia). Weed Sci 56:797–806 Google Scholar
Whaley, CM, Wilson, HP, Westwood, JH (2007) A new mutation in plant ALS confers resistance to five classes of ALS-inhibiting herbicides. Weed Sci 55:83–90 Google Scholar
Yang, Q, Deng, W, Wang, S, Liu, H, Li, X, Zheng, M (2018a). Effects of resistance mutations of Pro197, Asp376 and Trp574 on the characteristics of acetohydroxyacid synthase (AHAS) isozymes. Pest Manag Sci 74:1870–1879 Google Scholar
Yang, Q, Li, J, Shen, J, Xu, Y, Liu, H, Deng, W, Li, X, Zheng, M (2018b) Metabolic resistance to acetolactate synthase inhibiting herbicide tribenuron-methyl in Descurainia sophia L. mediated by cytochrome P450 enzymes. J Agric Food Chem 66:4319–4327 Google Scholar
Yu, Q, Han, H, Li, M, Purba, E, Walsh, MJ, Powles, SB (2012) Resistance evaluation for herbicide resistance-endowing acetolactate synthase (ALS) gene mutations using Raphanus raphanistrum accessions homozygous for specific ALS mutations. Weed Res 52:178–186 Google Scholar
Yu, Q, Han, H, Powles, SB (2008) Mutations of the ALS gene endowing resistance to ALS-inhibiting herbicides in Lolium rigidum populations. Pest Manag Sci 64:1229–1236 Google Scholar
Yu, Q, Zhang, XQ, Hashem, A, Walsh, MJ, Powles, SB (2003) ALS gene proline (197) mutations confer ALS herbicide resistance in eight separated wild radish (Raphanus raphanistrum) populations. Weed Sci 51:831–838 Google Scholar
Zhang, L, Guo, W, Li, Q, Wu, C, Zhao, N, Liu, W, Wang, J (2017) Tribenuron-methyl resistance and mutation diversity of the AHAS gene in shepherd’s purse (Capsella bursa-pastoris (L.) Medik.) in Henan Province, China. Pestic Biochem Physiol 143:239–245 Google Scholar
Zhang, LL, Li, Q, Guo, WL, Li, W, Wang, JX (2016) Molecular mechanism of Galium aparine resistance to tribenuron-methyl. Chinese Agricultural Science Bulletin 32:110–113. Chinese Google Scholar
Zhao, B, Fu, D, Yu, Y, Huang, C, Yan, K, Li, P, Shafi, J, Zhu, H, Wei, S, Ji, M (2017) Non-target-site resistance to ALS-inhibiting herbicides in a Sagittaria trifolia L. population. Pestic Biochem Physiol 140:79–84 Google Scholar