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The Tetrazolium Reaction as a Measure of the Action of Diquat in Elodea

Published online by Cambridge University Press:  12 June 2017

P. J. Davies  and
D. E. Seaman
P. J. Davies
Affiliation:
Department of Botany, University of California, Davis, California
D. E. Seaman
Affiliation:
Department of Botany, University of California, Davis, California
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Abstract

The production of red triphenyl formazan (hereinafter referred to as TPF) from colorless 2,3,5-triphenyl tetrazolium chloride (hereinafter referred to as TTC) by living cells was used as a vitality indicator for examining the effects of 6,7-dihydrodipyrido[1,2-a:2′,1′-c] pyrazidiinium salt (diquat) in elodea (Elodea canadensis Michx) shoots at varying light intensities. Diquat at 10 ppmw completely killed the elodea in 6 hr at 183 ft-c and at almost the same rate at 13,000 ft-c due to light saturation near the lower level. Over 24 hr were required for this effect in the dark. Between 29 and 183 ft-c, TPF production varied inversely with the light intensity. A comparison is made with the destruction of chlorophyll by diquat.

Type
Research Article
Information
Weed Science , Volume 16 , Issue 3 , July 1968 , pp. 329 - 331
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

1. Blackburn, R. D., Lawrence, J. M., and Davis, D. E. 1961. Effects of light intensity and quality on the growth of Elodea densa and Heteranthera dubia . Weeds 9:251–257.CrossRefGoogle Scholar
2. Calderbank, A. 1960. Diquat: A new herbicide and desiccant. Agr. Vet. Chem. 1:197–200.Google Scholar
3. Cronshey, J. F. H. 1961. A review of experimental work with diquat and related compounds. Weed Res. 1:68–77.Google Scholar
4. Currier, H. B. and Day, B. E. 1954. The tetrazolium reaction in yeast. Science 119:817.CrossRefGoogle ScholarPubMed
5. Currier, H. B. and Peoples, S. A. 1954. Phytotoxicity of hydrocarbons. Hilgardia 23:155–173.CrossRefGoogle Scholar
6. Currier, H. B. and van der Zweep, W. 1955. Plasmolysis and the tetrazolium reaction in Anacharis canadensis . Protoplasma 45:126–132.Google Scholar
7. Davenport, H. E. 1963. The mechanism of cyclic phosphorylation by illuminated chloroplasts. Proc. Roy. Soc. (B) 157:332–345.Google ScholarPubMed
8. Davies, P. J. and Seaman, D. E. 1964. Physiological effects of diquat on submersed aquatic weeds. WSA Abstr. p. 100.Google Scholar
9. Davies, P. J. and Seaman, D. E. 1968. Uptake and translocation of diquat in Elodea . Weed Science (In press).Google Scholar
10. Funderburk, H. H. Jr. and Lawrence, J. M. 1964. Mode of action and metabolism of diquat and paraquat. Weeds 12:259–264.CrossRefGoogle Scholar
11. Homer, R. F., Mees, G. C., and Tomlinson, G. C. 1960. Mode of action of dipyridyl quatenary salts as herbicides. J. Sci. Food Agr. 6:309–315.Google Scholar
12. Mees, G. C. 1960. Experiments on the herbicidal action of 1,1′-ethylene-2,2′—dipyridylum dibromide. Ann. Appl. Biol. 48:601–612.CrossRefGoogle Scholar
13. Smith, F. E. 1951. Tetrazolium Salt. Science 113:751–754.CrossRefGoogle ScholarPubMed