Hostname: page-component-78c5997874-dh8gc Total loading time: 0 Render date: 2024-11-13T02:57:46.295Z Has data issue: false hasContentIssue false
  • English
  • Français

Tolerance of Birdsfoot Trefoil (Lotus corniculatus) to 2,4-D

Published online by Cambridge University Press:  12 June 2017

Cynthia Davis  and
Dean L. Linscott
Cynthia Davis
Affiliation:
U.S. Dep. Agric., Agric. Res. Serv., Dep. Agron., Cornell Univ., Ithaca, NY 14853
Dean L. Linscott
Affiliation:
U.S. Dep. Agric., Agric. Res. Serv., Dep. Agron., Cornell Univ., Ithaca, NY 14853
Get access Rights & Permissions [Opens in a new window]

Abstract

Translocation and metabolism of 14C-2,4-D [(2,4-dichlorophenoxy)acetic acid] and effects of 2,4-D on protein synthesis were compared in ‘T–68’ (2,4-D tolerant) and ‘Viking’ (susceptible) birdsfoot trefoil (Lotus corniculatus L.) in an attempt to elucidate some tolerance mechanisms. After 14C-2,4-D was applied to upper trifoliate leaves, significantly less 2,4-D was found in stems, in leaves below the treated leaves, and in roots of T–68 compared to Viking. More 2,4-D was bound to alcohol-insoluble cellular constituents of T–68 leaves, stems, and roots. When alcohol-soluble components were fractionated, slightly more 14C water-soluble compounds were found in T–68, indicating further inactivation by glycosylation. No amino acid-2,4-D conjugates were found. The rate of 14CO2 evolution from 14C-2,4-D treated seedlings in T–68 was five times that in Viking. Protein synthesis appeared to be more rapid in T–68 but the relationship to 2,4-D was not clear. In part, 2,4-D resistance in T–68 may result from its ability to inactivate 2,4-D by differential binding and conjugation and by side chain breakdown as indicated by 14CO2 release.

Keywords

Herbicide metabolismherbicide degradationherbicide selectivityauxin
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 34 , Issue 3 , May 1986 , pp. 373 - 376
Copyright
Copyright © 1986 by the Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

1. Blacklow, W. M. and Linscott, D. L. 1968. The fate of 2,4-D applied to Viking birdsfoot trefoil and a resistant intercross. Weed Sci. 16:516–519.CrossRefGoogle Scholar
2. Davidonis, G. H., Hamilton, R. H., and Mumma, R. O. 1980. Comparative metabolism of 2,4-dichlorophenoxyacetic acid in cotyledon and leaf callus from two varieties of soybean. Plant Physiol. 65:94–97.CrossRefGoogle Scholar
3. Davidonis, G. H., Hamilton, R. H., and Mumma, R. O. 1982. Metabolism of 2,4-dichlorophenoxyacetic acid in 2,4-dichlorophenoxyacetic acid resistant soybean callus tissue. Plant Physiol. 70:104–107.CrossRefGoogle Scholar
4. Davidonis, G. H., Hamilton, R. H., Vallejo, R. P., Buly, R., and Mumma, R. O. 1982. Biological properties of D-amino acid conjugates of 2,4-D. Plant Physiol. 70:357–360.CrossRefGoogle Scholar
5. Devine, T. E., Seaney, R. R., Linscott, D. L., Hagin, R. D., and Brace, N. 1975. Results of breeding for tolerance to 2,4-D in birdsfoot trefoil. Crop Sci. 15:721–724.CrossRefGoogle Scholar
6. Hoagland, D. R. and Arnon, D. I. 1938. Univ. California Agric. Exp. Stn. Circular No. 347.Google Scholar
7. Luckwill, L. C. and Lloyd-Jones, C. P. 1960. Metabolism of plant growth regulators. I. 2,4-D in leaves of red and black currants. Ann. Appl. Biol. 48:613–625.CrossRefGoogle Scholar
8. Luckwill, L. C. and Lloyd-Jones, C. P. 1960. Metabolism of plant growth regulators. II. Decarboxylation of 2,4-D in leaves of apple and strawberry. Ann. Appl. Biol. 48:626–636.CrossRefGoogle Scholar
9. Murray, M. G. and Key, J. L. 1978. 2,4-dichlorophenoxyacetic acid-enhanced phosphorylation of soybean nuclear proteins. Plant Physiol. 61:190–198.CrossRefGoogle Scholar
10. Scheel, D. and Sanderman, H. 1981. Metabolism of 2,4-D in cell suspension cultures of soybean (Glycine max L.) and wheat (Triticum anestivum L.). Planta 152:253–258.CrossRefGoogle Scholar
11. Seaney, R. R., Heath, M. E., Metcalf, D. S., and Barnes, R. F. 1973. In Forages, the Science of Grassland Agriculture. Iowa State Univ. Press, Ames, IA. Page 178.Google Scholar
12. Swanson, E. B. and Tomes, D. T. 1980. In vitro responses of tolerant and susceptible lines of Lotus corniculatus L. to 2,4-D. Crop Sci. 20:792–795.CrossRefGoogle Scholar
13. Zurfluh, L. L. and Guilfoyle, T. J. 1980. Auxin-induced changes in the patterns of protein synthesis in soybean hypocotyl. Proc. Nat. Acad. Sci.: USA 77:357–361.CrossRefGoogle ScholarPubMed
14. Zurfluh, L. L. and Guilfoyle, T. J. 1982. Auxin-induced changes in the population of translatable messenger RNA in elongating sections of soybean hypocotyl. Plant Physiol. 69:332–337.CrossRefGoogle ScholarPubMed