Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-30T19:57:19.574Z Has data issue: false hasContentIssue false
  • English
  • Français

Use of Single Leaf Cells to Study Mode of Action of SAN 6706 on Soybean and Cotton

Published online by Cambridge University Press:  12 June 2017

Earl M. Porter  and
Paul G. Bartels
Earl M. Porter
Affiliation:
Dep. Biol. Sci., Univ. of Arizona, Tucson, AS 85721
Paul G. Bartels
Affiliation:
Dep. Biol. Sci., Univ. of Arizona, Tucson, AS 85721
Get access Rights & Permissions [Opens in a new window]

Abstract

Enzymatically isolated leaf cells from cotton (Gossypium hirsutum L. ‘Stoneville’) and soybean (Glycine max (L) Merr. ‘Kino’) were used to study the effect of SAN 6706 [4-chloro-5-(dimethylamino)-2-(α,α,α-trifluoro-m-tolyl)-3(2H)-pyridazinone] on photosynthesis, protein, RNA, and lipid synthesis during the first 19 hr of herbicidal treatment. Cotton plants are tolerant to SAN 6706, whereas soybean plants are susceptible. SAN 6706 inhibited the incorporation of 14C-bi-carbonate, uridine, and acetate into cotton leaf cells but stimulated incorporation of leucine. Incorporation of all of these precursors into soybean cells was inhibited. Cotton and soybean cells each took up equivalent amounts of labeled SAN 6706. The incorporation activity of the cotton and soybean cells appeared to regulate the entrance of the precursors into the cells by depleting the intracellular precursor pools. It is suggested that the inhibition of RNA and lipid synthesis in cotton and soybean cells is an indirect result of inhibited photosynthesis. Isolated cotton cells were not tolerant of the herbicide.

Type
Research Article
Information
Weed Science , Volume 25 , Issue 1 , January 1977 , pp. 60 - 65
Copyright
Copyright © 1977 by the Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

1. Bartels, P.G. and McCullough, C. 1972. A new inhibitor of carotenoid synthesis in higher plants: Sandoz 6706. Biochem. Biophys. Res. Commun. 45:16–22.CrossRefGoogle Scholar
2. Ben-Aziz, A. and Koren, E. 1974. Interference of carotenogenesis as a mechanism of action of the pyridiazinone herbicide Sandoz 6706. Plant Physiol. 54:916–920.Google Scholar
3. Boulware, M.A. and Camper, N.D. 1972. Effects of selected herbicides on plant protoplasts. Physiol. Plant. 26:313–317.Google Scholar
4. Francki, R.I.B., Zaitlin, M., and Jensen, R.G. 1971. Metabolism of separated leaf cells II uptake and incorporation of protein and ribonucleic acid precursors by tobacco cells. Plant Physiol. 48:14–18.Google Scholar
5. Hilton, J.L., Scharen, A.L., St. John, J.B., Moreland, D.E., and Norris, K.H. 1969. Modes of action of pyridazinone herbicides. Weed Sci. 17:541–547.Google Scholar
6. Hilton, J.L., St. John, J.B., Christiansen, M.N., and Norris, K.H. 1971. Interaction of lipoidal materials and a pyridazinone inhibitor of chloroplast development. Plant Physiol. 48:171–177.Google Scholar
7. Jensen, R.G., Francki, R.I.B., and Zaitlin, M. 1971. Preparation of photosynthetically active cells from tobacco. Plant Physiol. 48:9–13.Google Scholar
8. Rehfeld, D.W. and Jensen, R.G. 1973. Metabolism of separated cells. HI Effects of calcium and ammonium on product distribution. during photosynthesis with cotton cells. Plant Physiol. 52:17–22.CrossRefGoogle Scholar
9. Strang, R.H. and Rogers, R.L. 1974. Behavior and fate of two phenylpyridazinone herbicides in cotton, corn and soybean. J. Agric. Food Chem. 22:1119–1125.Google Scholar
10. St. John, J.B. and Christiansen, M.N. 1976. Inhibition of linolenic acid synthesis and modification of chilling resistance in cotton seedlings. Plant Physiol. 57:257–259.CrossRefGoogle Scholar