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Target and Nontarget Resistance Mechanisms Induce Annual Bluegrass (Poa annua) Resistance to Atrazine, Amicarbazone, and Diuron

Published online by Cambridge University Press:  20 January 2017

Andrej W. Svyantek ,
Phillipe Aldahir ,
Shu Chen ,
Michael L. Flessner ,
Patrick E. McCullough ,
Sudeep S. Sidhu  and
J. Scott McElroy
Andrej W. Svyantek
Affiliation:
Department of Horticulture, Auburn University, Auburn, AL 36849
Phillipe Aldahir
Affiliation:
Department of Crops, Soils, and Environmental Sciences, Auburn University, Auburn, AL 36849
Shu Chen
Affiliation:
Department of Grassland Science, College of Forestry and Architecture, South China Agricultural University, Guangzhou, China
Michael L. Flessner
Affiliation:
Department of Plant Pathology, Physiology, and Weed Science, Virginia Tech University, Blacksburg, VA 24061
Patrick E. McCullough
Affiliation:
Department of Crop and Soil Sciences, University of Georgia, Athens, GA 30602
Sudeep S. Sidhu
Affiliation:
Department of Crop and Soil Sciences, University of Georgia, Athens, GA 30602
J. Scott McElroy*
Affiliation:
Department of Crops, Soils, and Environmental Sciences, Auburn University, Auburn, AL 36849
*
∗Corresponding author's E-mail: jsm0010@auburn.edu
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Abstract

Annual bluegrass is a weed species in turfgrass environments known for exhibiting resistance to multiple herbicide modes of action, including photosystem II (PSII) inhibitors. To evaluate populations of annual bluegrass for susceptibility to PSII inhibitors of varied chemistries, populations were treated with herbicides from triazolinone, triazine, and substituted urea families: amicarbazone, atrazine, and diuron, respectively. Sequencing of the psbA gene confirmed the presence of a Ser264 to Gly amino acid substitution within populations that exhibited resistance to both atrazine and amicarbazone. A single biotype, DR3, which lacked any previously reported psbA gene point mutation, exhibited resistance to diuron, atrazine, and amicarbazone. DR3 had a significantly lower rate of absorption and translocation of atrazine and had enhanced atrazine metabolism when compared with both the Ser264 to Gly resistant mutant and susceptible biotypes. We thus report possible nontarget mechanisms of resistance to PSII-inhibiting herbicides in annual bluegrass.

Poa annua es una especie de maleza en ambientes de céspedes conocida por presentar resistencia a múltiples modos de acción de herbicidas, incluyendo inhibidores del fotosistema II (PSII). Para evaluar la susceptibilidad de poblaciones de P. annua a inhibidores PSII de diferentes características químicas, varias poblaciones fueron tratadas con herbicidas de las familias triazolinone, triazine, y urea sustituidas: amicarbazone, atrazine, y diuron, respectivamente. La secuenciación del gen psbA confirmó la presencia de una sustitución de amino ácidos de Ser264 a Gly en poblaciones que presentaron resistencia a atrazine y amicarbazone. Un solo biotipo, DR3, el cual carecía de cualquier reporte previo de mutaciones puntuales en el gen psbA, presentó resistencia a diuron, atrazine, y amicarbazone. DR3 tuvo una tasa de absorción y translocación significativamente menor de atrazine y un mayor metabolismo de atrazine cuando se comparó con biotipos resistentes con la mutación Ser264 a Gly y con biotipos susceptibles. De esta forma, reportamos posibles mecanismos de resistencia a herbicidas inhibidores de PSII en P. annua que no involucran el sitio activo.

Keywords

Amicarbazoneatrazinediuronannual bluegrass, Poa annua L.Herbicideherbicide resistanceresistancetarget-site mutationturfgrass
Type
Research Article
Information
Weed Technology , Volume 30 , Issue 3 , September 2016 , pp. 773 - 782
Copyright
Copyright © Weed Science Society of America 

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Footnotes

Associate editor for this paper: Andrew Kniss, University of Wyoming.

References

Literature Cited

Arias, R.S, Netherland, MD, Scheffler, BE, Puri, A, Dayan, FE (2005) Molecular evolution of herbicide resistance to phytoene desaturase inhibitors in Hydrilla verticillata and its potential use to generate herbicide-resistant crops. Pest Manag Sci 61: 258–268 Google Scholar
Brosnan, JT, Breeden, GK, Mueller, TC (2012) A glyphosate-resistant biotype of annual bluegrass in Tennessee. Weed Sci 60: 97–100 CrossRefGoogle Scholar
Brosnan, JT, Breeden, GK, Vargas, JJ, Grier, L (2015) A biotype of annual bluegrass (Poa annua) in Tennessee is resistant to inhibitors of ALS and photosystem II. Weed Sci 63: 321–328 CrossRefGoogle Scholar
Burgos, NR, Tranel, PJ, Streibig, JC, Davis, VM, Shaner, D, Norsworthy, JK, Ritz, C (2013) Review: confirmation of resistance to herbicides and evaluation of resistance levels. Weed Sci 61: 4–20.Google Scholar
Burnet, MWM, Loveys, BR, Holtum, JAM, Powles, SB (1993a) A mechanism of chlorotoluron resistance in Lolium rigidum . Planta 190: 182–189 Google Scholar
Burnet, MWM, Loveys, BR, Holtum, JAM, Powles, SB (1993b) Increased detoxification is a mechanism of simazine resistance in Lolium rigidum . Pestic Biochem Physiol 46: 207–218 CrossRefGoogle Scholar
Casler, MD, Duncan, RR (2003) Turfgrass Biology, Genetics, and Breeding. Hoboken, NJ: J. Wiley Google Scholar
Chodová, D, Mikulka, J, Koèová, M, Janáèek, J (1994) Different growth and photosynthetic efficiency of triazine resistant and susceptible biotypes of Poa annua L. Biol Plant (Prague) 36: 341 Google Scholar
Cutulle, MA, McElroy, JS, Millwood, RW, Sorochan, JC, Stewart, CN (2009) Selection of bioassay method influences detection of annual bluegrass resistance to mitotic-inhibiting herbicides. Crop Sci 49: 1088 Google Scholar
Darmency, H, Gasquez, J (1981) Inheritance of triazine resistance in Poa annua: Consequences for population dynamics. New Phytol 89: 487–493 CrossRefGoogle Scholar
Dayan, FE, Trindade, MLB, Velini, ED (2009) Amicarbazone, a new photosystem II inhibitor. Weed Sci 57: 579–583 CrossRefGoogle Scholar
Davis, DE, Gramlich, JV, Funderburk, HH Jr. (1965) Atrazine absorption and degradation by corn, cotton, and soybeans. Weeds 13: 252–255 Google Scholar
Délye, C (2013) Unravelling the genetic bases of non-target-site-based resistance (NTSR) to herbicides: a major challenge for weed science in the forthcoming decade. Pest Manag Sci 69: 176–187 Google Scholar
Délye, C, Jasieniuk, M, Le Corre, V (2013) Deciphering the evolution of herbicide resistance in weeds. Trends Genet 29: 649–658 CrossRefGoogle ScholarPubMed
De Prado, R., De Prado, JL, Menendez, J (1997) Resistance to substituted urea herbicides in Lolium rigidum biotypes. Pestic Biochem Physiol 57: 126–136 Google Scholar
De Prado, R, Menendez, J (1996) Management of herbicide-resistant grass weeds in Europe. Pages 393–398 in Brown, H, ed. Proceedings of the Second International Weed Control Congress. Copenhagen, Denmark: Department of Weed Control & Pesticide Ecology Google Scholar
Devine, MD, Shukla, A (2000) Altered target sites as a mechanism of herbicide resistance. Crop Prot 19: 881–889 Google Scholar
Gronwald, JW (1997) Resistance to PS II inhibitor herbicides. Pages 53–59 in de Prado, R, Jorrin, J, Garcia-Torres, L, eds. Weed and Crop Resistance to Herbicides. Dordrecht, Netherlands: Springer Google Scholar
Gronwald, JW, Andersen, RN, Yee, C (1989) Atrazine resistance in velvetleaf (Abutilon theophrasti) due to enhanced atrazine detoxification. Pestic Biochem Physiol 34: 149–163 Google Scholar
Hall, LM, Moss, SR, Powles, SB (1995) Mechanism of resistance to chlorotoluron in two biotypes of the grass weed Alopecurus myosuroides . Pestic Biochem Physiol 53: 180–192.CrossRefGoogle Scholar
Hanson, BD, Mallory-Smith, CA (2000). Diuron-resistant Poa annua is resistant to norflurazon. Weed Sci 48: 666–668 CrossRefGoogle Scholar
Heap, I (2015) The International Survey of Herbicide Resistant Weeds. http://www.weedscience.org. Accessed February 17, 2015Google Scholar
Hoagload, DR, Arnon, DI (1950) The Water-Culture Method for Growing Plants without Soil. 2nd edn. Berkeley, CA: College of Agriculture, University of California Circ 347. 32 pGoogle Scholar
Hutto, KC, Coats, GE, Taylor, JM (2004) Annual bluegrass (Poa annua) resistance to simazine in Mississippi. Weed Technol 18: 846–849 Google Scholar
Kelly, ST, Coats, GE, Luthe, DS (1999) Mode of resistance of triazine-resistant annual bluegrass (Poa annua). Weed Technol 13: 747–752 Google Scholar
Matzrafi, M, Gadri, Y, Frenkel, E, Rubin, B, Peleg, Z (2014) Evolution of herbicide resistance mechanisms in grass weeds. Plant Sci 229: 43–52 CrossRefGoogle ScholarPubMed
Mao, Q, Huff, DR (2012). The evolutionary origin of Poa annua L. Crop Sci 52: 1910–1922 CrossRefGoogle Scholar
McElroy, JS, Flessner, ML, Wang, Z, Dane, F, Walker, RH, Wehtje, GR (2013) A Trp 574 to Leu amino acid substitution in the ALS gene of annual bluegrass (Poa annua) is associated with resistance to ALS-inhibiting herbicides. Weed Sci 61: 21–25 Google Scholar
Menendez, J, Jorrin, J, Romera, E, Prado, RD (1994) Resistance to chlorotoluron of a slender foxtail (Alopecurus myosuroides) biotype. Weed Sci 42: 340–344 Google Scholar
Mengistu, LW, Mueller-Warrant, GW, Liston, A, Barker, RE (2000) psbA mutation (valine219 to isoleucine) in Poa annua resistant to metribuzin and diuron. Pest Manag Sci 56: 209–217 3.0.CO;2-8>CrossRefGoogle Scholar
Menne, H (2005) Classification of Herbicide Site of Action. HRAC Herbicide Resistance Action Committee. http://www.hracglobal.com/pages/classificationofherbicidesiteofaction.aspx. Accessed July 17, 2015Google Scholar
Netland, J (1996) Weed species and frequency of simazine-resistant populations of Poa annua and Senecio vulgaris in nursery stocks imported to Norway or inland-raised. Pages 461–468 in Brown, H, ed. Proceedings of the Second International Weed Control Congress. Copenhagen, Denmark: Department of Weed Control & Pesticide Ecology Google Scholar
Pan, G, Si, P, Yu, Q, Tu, J, Powles, S (2012) Non-target site mechanism of metribuzin tolerance in induced tolerant mutants of narrow-leafed lupin (Lupinus angustifolius L.). Crop Pasture Sci 63: 452–458 Google Scholar
Patzoldt, WL, Dixon, BS, Tranel, PJ (2003) Triazine resistance in Amaranthus tuberculatus (Moq) Sauer that is not site-of-action mediated. Pest Manag Sci 59: 1134–1142 Google Scholar
Patzoldt, WL, Hager, AG, McCormick, JS, Tranel, PJ (2006) A codon deletion confers resistance to herbicides inhibiting protoporphyrinogen oxidase. Proc Natl Acad Sci USA 103: 12329–12334 Google Scholar
Perry, DH, McElroy, JS, Dane, F, van Santen, E, Walker, RH (2012) Triazine-resistant annual bluegrass (Poa annua) populations with Ser264 mutation are resistant to amicarbazone. Weed Sci 60: 355–359 CrossRefGoogle Scholar
Powles, SB, Yu, Q (2010) Evolution in action: plants resistant to herbicides. Annu Rev Plant Biol. 61: 317–347 Google Scholar
Preston, C (2003) Inheritance and linkage of metabolism-based herbicide cross-resistance in rigid ryegrass (Lolium rigidum). Weed Sci 51: 4–12 Google Scholar
Preston, C, Powles, SB (1997) Light-dependent enhanced metabolism of chlorotoluron in a substituted urea herbicide-resistant biotype of Lolium rigidum Gaud. Planta 201: 202–208 CrossRefGoogle Scholar
Preston, C, Tardif, FJ, Christopher, JT, Powles, SB (1996) Multiple resistance to dissimilar herbicide chemistries in a biotype of Lolium rigidum due to enhanced activity of several herbicide degrading enzymes. Pestic Biochem Physiol 54: 123–134 CrossRefGoogle Scholar
Putwain, PD (1982). Herbicide resistance in weeds—an inevitable consequence of herbicide use. Pages 719–728 in Proceedings of the 1982 British Crop Protection Conference—Weeds. Farnham, UK: British Crop Protection Council.Google Scholar
Roeth, FW, Lavy, TL (1971) Atrazine uptake by sudangrass, sorghum and corn. Weed Sci 19: 93–97 Google Scholar
Singh, R, Sidhu, SS, McCullough, PE (2015) Physiological basis for triazine herbicide tolerance in bermudagrass, seashore paspalum, and zoysiagrass. Crop Sci 55: 2334–2341 Google Scholar
Smith, AE (1983) Differential bahiagrass (Paspalum notatum) cultivar response to atrazine. Weed Sci 31: 88–92 Google Scholar
Tian, X, Darmency, H (2006) Rapid bidirectional allele-specific PCR identification for triazine resistance in higher plants. Pest Manag Sci 62: 531–536 Google Scholar
Vargas, JM, Turgeon, AJ (2003) Poa annua: Physiology, Culture, and Control of Annual Bluegrass. Hoboken, NJ: J. Wiley Google Scholar
Yelverton, F, Isgrigg, J (1998) Herbicide-resistant weeds in turfgrass. Golf Course Manag 66: 56–61 Google Scholar
Yuan, JS, Tranel, PJ, Stewart, CN (2007) Non-target-site herbicide resistance: a family business. Trends Plant Sci 12: 6–13 Google Scholar
Yu, J, McCullough, PE, Vencill, WK (2013) Absorption, translocation, and metabolism of amicarbazone in annual bluegrass (Poa annua), creeping bentgrass (Agrostis stolonifera), and tall fescue (Festuca arundinacea). Weed Sci 61: 217–221 Google Scholar