Hostname: page-component-cd9895bd7-gxg78 Total loading time: 0 Render date: 2024-12-29T06:17:42.111Z Has data issue: false hasContentIssue false
  • English
  • Français

Physiological and Molecular Characterization of Atrazine Resistance in a Wild Radish (Raphanus raphanistrum) Population

Published online by Cambridge University Press:  20 January 2017

L. J. Shane Friesen  and
Stephen B. Powles
L. J. Shane Friesen
Affiliation:
Western Australian Herbicide Resistance Initiative, The School of Plant Biology, University of Western Australia, 35 Stirling Highway, Crawley, WA, Australia 6907
Stephen B. Powles*
Affiliation:
Western Australian Herbicide Resistance Initiative, The School of Plant Biology, University of Western Australia, 35 Stirling Highway, Crawley, WA, Australia 6907
*
Corresponding author's E-mail: spowles@plants.uwa.edu.au
Get access Rights & Permissions [Opens in a new window]

Abstract

This study documents the physiology and genetics of evolved atrazine resistance in a wild radish population from Western Australia. Plant response to atrazine treatment confirmed a high level of resistance in population WARR5. At 0.25 kg atrazine/ha, all plants from a susceptible population were killed, whereas resistant WARR5 was unaffected at the highest dose tested (4 kg atrazine/ha). Leaf photosynthesis in susceptible plants was inhibited after 1 kg atrazine/ha treatment, whereas leaf photosynthesis in WARR5 plants was unaffected. Furthermore, atrazine resistance was maternally inherited. Sequencing of a psbA gene fragment in resistant WARR5 and susceptible plants revealed a single point mutation resulting in a coding change from Ser264 to Gly of the D1 protein in resistant plants. We are confident that this mutation is the basis of resistance to the photosystem II inhibitors in this wild radish population.

Keywords

Atrazinemetribuzinwild radish, Raphanus raphanistrum L. RAPRAHerbicide resistancepsbA mutationtriazine resistance
Type
Research
Information
Weed Technology , Volume 21 , Issue 4 , December 2007 , pp. 910 - 914
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

Anderson, M. P. and Gronwald, J. W. 1991. Atrazine resistance in a velvetleaf (Abutilon theophrasti) biotype due to enhanced glutathione S-transferase activity. Plant Physiol. 96:104–109.Google Scholar
Burnet, M. W. M., Loveys, B. R., Holtum, J. A. M., and Powles, S. B. 1993a. A mechanism of chlorotoluron resistance in Lolium rigidum . Planta. 190:182–189.Google Scholar
Burnet, M. W. M., Loveys, B. R., Holtum, J. A. M., and Powles, S. B. 1993b. Increased detoxification is a mechanism of simazine resistance in Lolium rigidum . Pestic. Biochem. Physiol. 46:207–218.Google Scholar
Christoffers, M. J., Nandula, V. K., Mengistu, L. W., and Messersmith, C. G. 2004. Altered herbicide target sites: Implications for herbicide-resistant weed management. Pages 199–210. in Inderjit, ed. Weed Biology and Management. New York Kluwer Academic.Google Scholar
Devine, M. D. and Eberlein, C. V. 1997. Physiological, biochemical and molecular aspects of herbicide resistance based on altered target sites. Pages 159–185. in Roe, R.M., Burton, J.D., Kuhr, R.J. eds. Herbicide Activity: Toxicology, Biochemistry and Molecular Biology. Fairfax, VA IOS.Google Scholar
Devine, M. D. and Shukla, A. 2000. Altered target sites as a mechanism of herbicide resistance. Crop Prot. 19:881–889.Google Scholar
Doyle, J. J. and Doyle, J. L. 1990. Isolation of plant DNA from fresh tissue. Focus 12:13–15.Google Scholar
Fraga, M. I. and Tasende, M. G. 2003. Mechanisms of resistance to simazine in Sonchus oleraceus . Weed Res. 43:333–340.Google Scholar
Gray, J. A., Balke, N. E., and Stoltenberg, D. E. 1996. Increased glutathione conjugation of atrazine confers resistance in a Wisconsin velvetleaf (Abutilon theophrasti) biotype. Pestic. Biochem. Physiol. 55:157–171.Google Scholar
Gressel, J. 2002. Molecular biochemistry of resistances that have evolved in the field. Pages 122–218. in. Molecular Biology of Weed Control. London Taylor and Francis.Google Scholar
Gressel, J., Regev, Y., Malkin, S., and Kleifeld, Y. 1983. Characterization of an s-triazine-resistant biotype of Brachypodium distachyon . Weed Sci. 31:450–456.Google Scholar
Gronwald, J. W. 1994. Resistance to photosystem II inhibiting herbicides. Pages 27–60. in Powles, S.B., Holtum, J.A.M. eds. Herbicide Resistance in Plants: Biology and Biochemistry. Boca Raton, FL Lewis.Google Scholar
Gronwald, J. W., Andersen, R. N., and Yee, C. 1989. Atrazine resistance in velvetleaf (Abutilon theophrasti) due to enhanced atrazine detoxification. Pestic. Biochem. Physiol. 34:149–163.CrossRefGoogle Scholar
Hashem, A., Dhammu, H. S., Powles, S. B., Bowran, D. G., Piper, T. J., and Cheam, A. H. 2001. Triazine resistance in a biotype of wild radish (Raphanus raphanistrum) in Australia. Weed Technol. 15:636–641.Google Scholar
Jasieniuk, M., Brule-Babel, A. L., and Morrison, I. N. 1996. The evolution and genetics of herbicide resistance in weeds. Weed Sci. 44:176–193.Google Scholar
Kimura, M. 1968. Genetic variability maintained in a finite population due to mutational production of neutral and nearly neutral isoalleles. Genet. Res. 11:247–269.Google Scholar
Kimura, M. and Crow, J. F. 1964. The number of alleles that can be maintained in a finite population. Genetics 49:725–738.Google Scholar
Kimura, M. and Ohta, T. 1973. The age of a neutral mutant persisting in a finite population. Genetics 75:199–212.Google Scholar
Lewontin, R. C. 1974. The paradox of variation. Pages 189–272. in. The Genetic Basis of Evolutionary Change. New York Columbia University Press.Google Scholar
Patzoldt, W. L., Dixon, B. S., and Tranel, P. J. 2003. Triazine resistance in Amaranthus tuberculatus (Moq) Sauer that is not site-of-action mediated. Pest. Manag. Sci. 59:1134–1142.Google Scholar
Plowman, A. M., Richards, A. J., and Tremayne, M. A. 1999. Environmental effects on the fitness of triazine-resistant and triazine-susceptible Brassica rapa and Chenopodium album in the absence of herbicide. New Phytol. 141:471–485.Google Scholar
Preston, C. and Mallory-Smith, C. A. 2001. Biochemical mechanisms, inheritance, and molecular genetics of herbicide resistance in weeds. Pages 23–60. in Powles, S.B., Shaner, D.L. eds. Herbicide Resistance and World Grains. Boca Raton, FL CRC.CrossRefGoogle Scholar
Shimabukuro, R. H., Lamoureux, G. L., and Frear, D. S. 1978. Glutathione conjugation: a mechanism of herbicide detoxication and selectivity in plants. Pages 133–149. in Pallos, F.M., Casida, J.E. eds. Chemistry and Action of Herbicide Antidotes. New York Academic.CrossRefGoogle Scholar
Thompson, J. D., Higgins, D. G., and Gibson, T. J. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673–4680.Google Scholar
Tian, X. and Darmency, H. 2006. Rapid bidirectional allele-specific PCR identification for triazine resistance in higher plants. Pest Manag. Sci. 62:531–536.Google Scholar
Walsh, M. J., Powles, S. B., Beard, B. R., Parkin, B. T., and Porter, S. A. 2004. Multiple-herbicide resistance across four modes of action in wild radish (Raphanus raphanistrum). Weed Sci. 52:8–13.Google Scholar
Wang, T., Li, Y., Shi, Y., Reboud, X., Darmency, H., and Gressel, J. 2004. Low frequency transmission of a plastid encoded trait in Setaria italica . Theor. Appl. Genet. 108:315–320.Google Scholar