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A Rapid Method to Determine Cereal Plant Response to Glyphosate

Published online by Cambridge University Press:  20 January 2017

M. C. Escorial ,
H. Sixto ,
J. M. García-Baudín  and
M. C. Chueca
M. C. Escorial
Affiliation:
Departamento Proteccion Vegetal, INIA, Carretera La Coruña Km 7, Madrid 28040, Spain
H. Sixto
Affiliation:
Departamento Proteccion Vegetal, INIA, Carretera La Coruña Km 7, Madrid 28040, Spain
J. M. García-Baudín
Affiliation:
Departamento Proteccion Vegetal, INIA, Carretera La Coruña Km 7, Madrid 28040, Spain
M. C. Chueca*
Affiliation:
Departamento Proteccion Vegetal, INIA, Carretera La Coruña Km 7, Madrid 28040, Spain
*
Corresponding author's E-mail: chueca@inia.es.
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Abstract

A rapid method for determining the response of cereals to glyphosate is described. This method detects the differential responses of plants in 4 d, allowing for the rapid selection of glyphosate-tolerance response. Two types of tests determined the efficacy of this rapid method: differential response to different dosages of herbicide in a coleoptile growth test and in sprayed plants. In seedling assay, barley cultivars showed a higher level of tolerance to glyphosate (the dose that causes 50% of the total effect [I50] = 0.066 and I50 = 0.060 mM) than wheat cultivars (I50 = 0.018 and I50 = 0.014 mM). The response in seedling assay is well correlated (r2 = 0.95 and r2 = 0.98) with the response in plant-sprayed assays. The method was employed to verify the tolerance level of a sensitive barley cultivar and the four tolerant and sensitive mutant lines derived from it.

Keywords

Glyphosatebarley, Hordeum vulgare L.wheat, Triticum aestivum L.Herbicide responseAN, Amaji NijoANOVA, analysis of varianceCS, Chinese SpringDAT, days after treatmentI50, dose that causes 50% of the total effect
Type
Research
Information
Weed Technology , Volume 15 , Issue 4 , December 2001 , pp. 697 - 702
Copyright
Copyright © Weed Science Society of America 

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Footnotes

Current address of second author: Departamento Silvopascicultura, INIA, Carretera La Coruña Km 7, Madrid 28040, Spain

References

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