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A Seed Bioassay to Identify Acetyl-CoA Carboxylase Inhibitor Resistant Wild Oat (Avena fatua) Populations

Published online by Cambridge University Press:  12 June 2017

Bruce G. Murray ,
Lyle F. Friesen ,
Kelly J. Beaulieu  and
Ian N. Morrison
Bruce G. Murray
Affiliation:
Dep. Plant Sci., Univ. Manitoba, Winnipeg, Manitoba, Canada, R3T 2N2
Lyle F. Friesen
Affiliation:
Dep. Plant Sci., Univ. Manitoba, Winnipeg, Manitoba, Canada, R3T 2N2
Kelly J. Beaulieu
Affiliation:
Dep. Plant Sci., Univ. Manitoba, Winnipeg, Manitoba, Canada, R3T 2N2
Ian N. Morrison
Affiliation:
Dep. Plant Sci., Univ. Manitoba, Winnipeg, Manitoba, Canada, R3T 2N2
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Abstract

A seed bioassay was developed and tested for the rapid identification of aryloxyphenoxypropionate (APP) and cyclohexanedione (CHD) resistance in wild oat. Two susceptible (S) genotypes, UM5 and Dumont, were treated with fenoxaprop-P and sethoxydim over a range of dosages on filter paper and agar. The former is a wild oat line and the latter a tame oat cultivar. Within 5 d, shoot and root development of both genotypes were completely inhibited by 10 μM fenoxaprop-P and 5 μM sethoxydim. These dosages were then tested to determine if they were suitable for distinguishing between resistant (R) and susceptible (S) plants. Agar medium was preferred over filter paper because of the ease of preparation and maintenance. Four known R wild oat populations were included in the tests. Those with high levels of resistance produced significantly longer coleoptiles and roots than S genotypes, but those with moderate or low levels of resistance could not be separated statistically from S biotypes based on quantitative measurements. However, after exposing the germinating, treated seeds to light for 24 to 48 h, all the R populations produced green coleoptiles and initiated a first leaf, unlike the S genotypes which did not turn green or produce any new growth. This procedure proved useful in discriminating between R and S genotypes and in ranking populations in terms of relative levels of resistance.

Keywords

Fenoxaprop-p-ethyl,(R)-2-[4-[(6-chloro-2-benzoxazolyl)oxy]phenoxy]propanoic acid, ethyl estersethoxydim, 2-[1-(ethoxyimino)butyl]-5-[2-(ethylthio)propyl]-3-hydroxy-2-cyclohexen-1-onewild oat, Avena fatua L. #3 AVEFAoat, Avena sativa L. # AVESAACCase inhibitorherbicide resistancefenoxaprop-p-ethylsethoxydimaryloxyphenoxypropionatecyclohexanedioneAVEFA
Type
Research
Information
Weed Technology , Volume 10 , Issue 1 , March 1996 , pp. 85 - 89
Copyright
Copyright © 1996 by the Weed Science Society of America 

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References

Literature Cited

1. Beckie, H. J., Friesen, L. F., Nawolsky, K. M., and Morrison, I. N. 1990. A rapid bioassay to detect trifluralin-resistant green foxtail (Setaria viridis). Weed Technol. 4:505–508.CrossRefGoogle Scholar
2. Brain, P. and Cousens, R., 1989. An equation to describe dose responses where there is stimulation of growth at low doses. Weed Res. 29:93–96.Google Scholar
3. Freund, R. J. and Littell, R. C. 1986. p. 133–141 in SAS System for Regression. SAS Inst. Inc., Cary, NC.Google Scholar
4. Gomez, K. A. and Gomez, A. A. 1984. p. 187–207 in Statistical Procedures for Agricultural Research. 2nd ed. John Wiley & Sons, New York.Google Scholar
5. Heap, I. M. 1994. Identification and documentation of herbicide resistance. Phytoprotection 75(Suppl.)85–90.Google Scholar
6. Heap, I. M. and Morrison, I. N. 1996. Resistance to aryloxyphenoxypropionate and cyclohexanedione herbicides in green foxtail (Setaria viridis). Weed Sci. (in press).Google Scholar
7. Heap, I. M., Murray, B. G., Loeppky, H. A., and Morrison, I. N. 1993. Resistance to aryloxyphenoxypropionate and cyclohexanedione herbicides in wild oat (Avena fatua). Weed Sci. 41:232–238.CrossRefGoogle Scholar
8. Koutsoyiannis, A. 1977. p. 81–91 in Theory of Econometrics. 2nd ed. MacMillan Education Ltd., London, U.K. CrossRefGoogle Scholar
9. Morrison, I. N. and Devine, M. D. 1994. Herbicide resistance in the Canadian prairie provinces: Five years after the fact. Phytoprotection 75(Suppl.): 5–16.CrossRefGoogle Scholar
10. Weed Science Society of America. 1994. Herbicide Handbook. 7th ed. Weed Sci. Soc. Am., Champaign, IL. p. 266–268.Google Scholar