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Viability and in Vitro Germination of Johnsongrass (Sorghum Halepense) Pollen

Published online by Cambridge University Press:  20 January 2017

Ian C. Burke ,
John W. Wilcut  and
Nina S. Allen
Ian C. Burke*
Affiliation:
Crop Science Department, North Carolina State University, Raleigh, NC 27695-7620
John W. Wilcut
Affiliation:
Crop Science Department, North Carolina State University, Raleigh, NC 27695-7620
Nina S. Allen
Affiliation:
Botany Department, Box 7620, North Carolina State University, Raleigh, NC 27695-7620
*
Corresponding author's E-mail: icburke@wsu.edu
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Abstract

A high proportion of viable pollen grains must germinate to study the physiology of pollen growth to reduce the confounding effects of environmental influences on pollen germination. The objectives of this study were to evaluate the nuclear state and develop a suitable medium and culture method for in vitro germination of johnsongrass pollen. Johnsongrass pollen was trinucleate, and in vitro tests for pollen viability using Alexander's stain and a fluorochromatic reaction method (FCR) indicated johnsongrass pollen was viable (92.6 to 98.4%). A factorial treatment arrangement of four concentrations of sucrose, two concentrations of boric acid, and two concentrations of calcium nitrate were used to determine the optimum pollen-germination medium composition in suspension culture, agar culture, and cellophane membrane culture. Germination was highest in a suspension culture with a medium containing 0.3 M sucrose, 2.4 mM boric acid, and 3 mM calcium nitrate. Pollen germination using this medium was 78.9% when anthers were harvested just before anthesis.

Keywords

Johnsongrass, Sorghum halepense (L.) Pers. SORHAPollen testsagar culturesuspension culturecellophane membrane culture
Type
Research
Information
Weed Technology , Volume 21 , Issue 1 , March 2007 , pp. 23 - 29
Copyright
Copyright © Weed Science Society of America 

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