Published online by Cambridge University Press: 12 June 2017
The action of trichloroacetate, a herbicide of grass-type plants such as quack grass and Johnson grass, has been studied for several years by observing the morphological responses of treated plants. Its presence in such plants has been indicated by analyses for the trichloromethyl group with a colorimetric test using pyridine and sodium hydroxide (1, 10). A review by Barrons and Hummer (1) indicates that root absorption is a much more important avenue of entry than foliar absorption. They also report differences in TCA content in the expressed sap of several species of treated plants. At 20 lbs. per acre they found “very little” TCA in corn and beans (both TCA-susceptible). At 10 lbs. per acre, they found “not a trace of TCA” in corn, “very little” in beans, but “a relatively high concentration of TCA” in peas (a relatively TCA-tolerant plant). However, Tibbitts and Holm (10) found 100 to 500 μg. of TCA per g. of fresh snap bean tissue from plants whose culture solutions contained 15 mg. of commercial grade 80% TCA as sodium salt per 150 ml. of nutrient. In order to check these observations, and to see whether TCA itself is present in the plants, and if so to determine the pattern of its distribution within the plants, as well as to look for possible metabolic products, C14-labeled TCA was synthesized and used in tracer experiments with corn and pea plants.