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Manipulation of egg yolk cholesterol: a physiologist's view

Published online by Cambridge University Press:  18 September 2007

H.D. Griffin
H.D. Griffin
Affiliation:
Department of Cellular and Molecular Biology, AFRC Institute of Animal Physiology and Genetics Research, Edinburgh Research Station, Roslin, Midlothian EH25 9PS, UK
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Abstract

Concern about the cholesterol content of the human diet has led to many attempts to reduce the cholesterol content of eggs. Although this has involved a number of different approaches, including genetic selection and nutritional or pharmacological manipulation, the overwhelming evidence is that egg yolk cholesterol level is very resistant to change. The present review argues that this is because of the particular mechanisms involved in yolk formation. Yolk precursors are synthesized in the liver of the laying hen and transported in the plasma to the ovary where they are taken up into the developing follicles by receptor-mediated endocytosis. As a consequence, the cholesterol content of the yolk is primarily dependent on the cholesterol content of triglyceride-rich lipoproteins. Studies in mammals have shown that inhibition of cholesterol synthesis can reduce the rate of synthesis and secretion of lipoproteins by the liver, but has little effect on the composition of the lipoproteins that are secreted. This response, and the key role of cholesterol in the synthesis of steroid hormones, would seem to preclude the possibility of any substantial reductions in egg yolk cholesterol levels through manipulation of cholesterol metabolism. Possible alternative approaches are briefly discussed.

Keywords

Eggcholesterolendocytosislipoprotein metabolismmanipulationyolkyolk formation
Type
Research Article
Information
World's Poultry Science Journal , Volume 48 , Issue 2 , July 1992 , pp. 101 - 112
Copyright
Copyright © Cambridge University Press 1992

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References

Arad, Y., Ramakrishnan, R. and Ginsberg, H.N. (1990) Lovastatin therapy reduces low density lipoprotein apo-B levels in subjects with combined hyperlipidemia by reducing the production of apo-B containing lipoproteins: implications for the pathophysiology of apo-B production. Journal of Lipid Research 31: 567–582CrossRefGoogle Scholar
Bacon, W.L. (1990) Metabolism and ovarian follicular uptake of the major yolk precursor in laying turkey hens. Poultry Science 65: 1184–1192CrossRefGoogle Scholar
Bacon, W.L., Leclercq, B. and Blum, J.-C. (1978) Differences in metabolism of very low density lipoprotein from laying chicken hens in comparison to immature chicken hens. Poultry Science 57: 1675–1686CrossRefGoogle ScholarPubMed
Bagdade, J.D., Lane, J.T., Stone, N., Ritter, M.C. and Subbaiah, P.V. (1990) Persistent abnormalities in lipoprotein composition and cholesteryl ester transfer following lovastatin treatment. Journal of Lipid Research 31: 1263–1269CrossRefGoogle ScholarPubMed
Beyer, R.S. and Jensen, L.S. (1989) Cholesterol content in commercially produced eggs in Georgia. Poultry Science 68: 1703–1706CrossRefGoogle Scholar
Bjorkem, I. and Skrede, S. (1989) Familial disease with storage of sterols other than cholesterol: cerebrotendinous xanthomatosis and phytosterolemia. In: The Metabolic Basis of Inherited Disease (Eds. Scriver, C.R., Beaudet, A.L., Sly, W.S. and Valle, D.), McGraw-Hill New York, pp. 1293–1302Google Scholar
Brown, W.V. (1990) Dietary recommendations to prevent coronary heart disease. Annals of the New York Academy of Science 598: 376–388CrossRefGoogle ScholarPubMed
Burgess, T.L., Burgess, C. and Wilson, J.D. (1962) Effect of MER-29 on egg production in chickens. Proceedings of the Society for Experimental Biology and Medicine 109: 218–221CrossRefGoogle Scholar
Cannon, G. (1990) Healthy Eating: The Experts Agree. HMSO, LondonGoogle Scholar
Cianflone, K.M., Vu, H., Bilodeau, M., Davignon, J. and Sniderman, A.D. (1990a) Modulation of chylomicron remnant metabolism by an HMG-CoA reductase inhibitor. Metabolism 39: 274–280CrossRefGoogle Scholar
Cianflone, K.M., Yasruel, Z., Rodriguez, M.A., Vas, D. and Sniderman, A.D. (1990b) Regulation of apo-B secretion from HepG2 cells: evidence for a critical role for cholesteryl ester synthesis in the response to a fatty acid challenge. Journal of Lipid Research 31: 2045–2055CrossRefGoogle ScholarPubMed
Clarkson, T.B. and Lofland, H.B. (1961) Effect of cholesterol-fat diets on pigeons susceptible and resistant to atherosclerosis. Circulation Research 9: 106–109CrossRefGoogle ScholarPubMed
Cook, W.H. (1968) Macromolecular components of egg yolk. In: Egg Quality: A Study of the Hen's Egg (Ed. Carter, T.C.), Oliver and Boyd, Edinburgh, pp. 109–132Google Scholar
Cunningham, D.L., Krueger, W.F., Fanguy, R.C. and Bradley, J.W. (1974) Preliminary results of bidirectional selection for yolk cholesterol in laying hens. Poultry Science 53: 384–391CrossRefGoogle ScholarPubMed
Elkin, R.G. and Rogler, J.C. (1989) Effect of lovastatin on laying hen performance and egg cholesterol content. Poultry Science 68 (Suppl. 1): 49Google Scholar
George, R., Barber, D.L. and Schneider, W.J. (1987) Characterisation of the chicken oocyte receptor for low and very low density lipoproteins. Journal of Biological Chemistry 262: 16838–16847CrossRefGoogle ScholarPubMed
Griffin, H.D. and Perry, M.M. (1985) Exclusion of plasma lipoproteins of intestinal origin from avian egg yolk because of their size. Comparative Biochemistry and Physiology 82B 321–325Google Scholar
Griffin, H.D., Perry, M.M. and Gilbert, A.B. (1985) Yolk formation. In: Physiology and Biochemistry of the Domestic Fowl (Ed. Freeman, B.), Academic Press, London, pp. 345–380Google Scholar
Hamilton, R.A., Moorehouse, A. and Havel, R.J. (1991) Isolation and properties of nascent lipoproteins from highly purified rat hepatocytic Golgi fractions. Journal of Lipid Research 32: 529–543CrossRefGoogle ScholarPubMed
Hargis, P.S. (1988) Modifying egg yolk cholesterol – a review. World's Poultry Science Journal 44: 17–29CrossRefGoogle Scholar
Harris, P.C. and Wilcox, F.H. (1963) Studies on egg yolk cholesterol. 3. Effect of dietary cholesterol. Poultry Science 42: 186–189CrossRefGoogle Scholar
Hayashi, K., Nimpf, J. and Schneider, W.J. (1989) Chicken oocytes and fibroblasts express different apolipoprotein-B-specific receptors. Journal of Biological Chemistry 264: 3131–3139CrossRefGoogle Scholar
Hidaka, H., Nakamura, T., Aoki, T. et al. (1990) Increased plasma plant sterol levels in heterozygotes with sitosterolemia and xanthomatosis. Journal of Lipid Research 31: 881–888CrossRefGoogle ScholarPubMed
Kahn, B., Wilcox, H.B. and Heimberg, M. (1989) Cholesterol is required for secretion of very low density lipoprotein by rat liver. Biochemical Journal 258: 807–816CrossRefGoogle Scholar
Lambson, R.O. (1980) An electron microscopic study of the endodermal cells of the yolk sac of the chick during incubation and after hatching. American Journal of Anatomy 129: 1–20CrossRefGoogle Scholar
Marks, H.L. and Washburn, K.W. (1977) Divergent selection for yolk cholesterol in laying hens. British Poultry Science 18: 179–188CrossRefGoogle ScholarPubMed
Miller, K.W. and Lane, M.D. (1984) Estradiol-induced alteration of very-low-density-lipoprotein assembly. Journal of Biological Chemistry 259: 15277–15286CrossRefGoogle ScholarPubMed
Mooney, R.A. and Lane, M.D. (1981) Formation and turnover of triglyceride-rich vesicles in chick liver cell. Journal of Biological Chemistry 256: 11724–11733CrossRefGoogle ScholarPubMed
Naber, E.C. (1976) The cholesterol problem, the egg and lipid metabolism in the laying hen. Poultry Science 55: 14–30CrossRefGoogle ScholarPubMed
Nimpf, J., George, R. and Schneider, W.J. (1988) Apolipoprotein specificity of the chicken oocyte receptor for low and very low density lipoproteins: lack of recognition of apolipoprotein VLDL-II. Journal of Lipid Research 29: 657–667CrossRefGoogle ScholarPubMed
Nimpf, J., Radosavljevic, M. and Schneider, W.J. (1989) Specific postendocytic proteolysis of apolipoprotein B in oocytes does not abolish receptor recognition. Proceedings of the National Academy of Science, USA 86: 906–910CrossRefGoogle Scholar
Noble, R.C. (1987) Egg lipids. In: Egg Quality – Current Problems and Recent Advances (Eds. Wells, R.G. and Belyavin, C.J.), Butterworths, London, pp. 159–177Google Scholar
Noble, R.C. and Cocchi, M. (1990) Lipid metabolism in the neonatal chicken. Progress in Lipid Research 29: 107–140CrossRefGoogle ScholarPubMed
Overturf, M.L., Smith, S.A., Gotto, A.M. Jr. et al. (1990) Dietary cholesterol absorption, and sterol and bile acid excretion in hypercholesterolemic-resistant white rabbits. Journal of Lipid Research 31: 2019–2027CrossRefGoogle ScholarPubMed
Page, T.G., Southern, L.L., Hebert, J.A., Ward, T.L. and Achee, V.N. (1991) Effect of chromium picolinate on serum cholesterol, egg production, egg cholesterol and egg quality of laying hens. Poultry Science 70: 91.Google Scholar
Parks, J.S., Wilson, M.D., Johnson, F.L. and Rudel, L.L. (1989) Fish oil decreases hepatic cholesteryl ester secretion but not apo-B secretion in African green monkeys. Journal of Lipid Research 30: 1535–1544CrossRefGoogle Scholar
Perry, M.M. and Gilbert, A.B. (1979) Yolk transport in the ovarian follicle of the hen (Gallus domesticus): lipoprotein like particles at the periphery of the oocyte in the rapid growth phase. Journal of Cell Science 39: 257–272CrossRefGoogle ScholarPubMed
Perry, M.M., Griffin, H.D. and Gilbert, A.B. (1985) The binding of very low density and low density lipoproteins to the plasma membrane of the hen's oocyte. A morphological study. Experimental Cell Research 151: 433–446CrossRefGoogle Scholar
Phillips, M.C., Johnson, W.J. and Rothblat, G.H. (1987) Mechanisms and consequences of cellular cholesterol exchange and transfer. Biochimica Biophysica Acta 906: 223–276CrossRefGoogle ScholarPubMed
Reinhart, M.P. (1990) Intracellular sterol trafficking. Experentia 46: 599–611CrossRefGoogle ScholarPubMed
Schneider, W.J., Carroll, R., Severson, D.L. and Nimpf, J. (1990) Apolipoprotein VLDL-II inhibits lipolysis of triglyceride-rich lipoproteins in the laying hen. Journal of Lipid Research 31: 507–513CrossRefGoogle ScholarPubMed
Stifani, S., George, R. and Schneider, W.J. (1987) Solubilisation and characterisation of the chicken oocyte vitellogenin receptor. Biochemical Journal 250: 467–475CrossRefGoogle Scholar
Stifani, S., Barber, D.L., Nimpf, J. and Schneider, W.J. (1990) A single chicken oocyte plasma membrane protein mediates uptake of very low density lipoprotein and vitellogenin. Proceedings of the National Academy of Science, USA 87: 1955–1959CrossRefGoogle ScholarPubMed
Tarugi, P., Regianni, D., Ottaviani, E., Ferrari, S., Tiozzo, R. and Calandra, S. (1989) Plasma lipoproteins, tissue cholesterol overload and skeletal muscle apolipoprotein A-I synthesis in the developing chick. Journal of Lipid Research 30: 9–22CrossRefGoogle ScholarPubMed
Tobert, J.A. (1987) New developments in lipid lowering therapy: the role of inhibitors of hydroxymethylglutaryl-coenzyme A reductase. Circulation 76: 534–538CrossRefGoogle ScholarPubMed
Vance, J.E. and Vance, D.E. (1990) The assembly of lipids into lipoproteins during secretion. Experentia 46: 560–569CrossRefGoogle ScholarPubMed
Van Elswyk, M.E., Schake, L.S. and Hargis, P.S. (1991) Evaluation of two extraction methods for the determination of egg yolk cholesterol. Poultry Science 70: 1258–1260CrossRefGoogle ScholarPubMed
Van Meer, G. (1989) Lipid traffic in animal cells. Annual Review of Cell Biology 5: 247–275CrossRefGoogle ScholarPubMed
Washburn, K.W. and Marks, H.L. (1977) Changes in fitness traits associated with selection for divergence in egg yolk cholesterol concentration. British Poultry Science 18: 188–199CrossRefGoogle Scholar
Wiskocil, R., Bensky, P., Dower, W., Goldberger, R.F., Gordon, J.I. and Deeley, R.G. (1980) Co-ordinated regulation of two estrogen-specific genes in avian liver. Proceedings of the National Academy of Science, USA 77: 4474–4478CrossRefGoogle Scholar
Yaffee, M., Schutz, H., Stone, J., Bokhari, S. and Zeidler, G. (1991) Consumer perception and utilisation of eggs and egg products. Poultry Science 70: 188Google Scholar