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The application of the microalgae Chlorella spp. as a supplement in broiler feed

Published online by Cambridge University Press:  05 April 2019

S.A. ABDELNOUR ,
M.E. ABD EL-HACK Open the ORCID record for M.E. ABD EL-HACK [Opens in a new window] ,
M. ARIF ,
A.F. KHAFAGA  and
A.E. TAHA
S.A. ABDELNOUR
Affiliation:
Department of Animal Production, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt
M.E. ABD EL-HACK*
Affiliation:
Department of Poultry, Faculty of Agriculture, Zagazig University, Zagazig 44511, Egypt
M. ARIF
Affiliation:
Department of Animal Sciences, College of Agriculture, University of Sargodha 40100, Pakistan
A.F. KHAFAGA
Affiliation:
Department of Pathology, Faculty of Veterinary Medicine, Alexandria University, Edfina 22758, Egypt
A.E. TAHA
Affiliation:
Department of Animal Husbandry and Animal Wealth Development, Faculty of Veterinary Medicine, Alexandria University, Edfina 22578, Egypt
*
Corresponding author: m.ezzat@zu.edu.eg; dr.mohamed.e.abdalhaq@gmail.com
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Abstract

Chlorella (vulgaris spp.; CLV) is a genus of unicellular freshwater microalgae that are fit for human consumption and are used as additives with high nutritional value in feed for agriculturally important animals. Chlorella spp. are characterised by their simple cultivation, high productivity and levels of protein and other nutrients. Investigations have shown that the growth performance of broilers can be positively affected by the addition of very low amounts of CLV biomass (0.5-1.0% of the diet) to feed. The effect of CLV on growth and development is considered to stem from its high protein content (60.6%) and nutritional value. Results have shown enhanced body weight gain (2.7%), better feed conversion ratio (lowered by 2.8%), meat colour and breast muscle weight (20.1%) in CLV-supplemented chicks compared to control birds (control breast weight 19.1%). Additionally, a significant decrease in drip loss (2.26%) from breast muscle was observed with CLV supplementation and levels of blood total protein, albumin, and high-density lipoprotein (HDL) cholesterol significantly increased (P<0.05), while the levels of liver enzymes indicative of oxidative damage (alanine aminotransferase, ALT) decreased by 23.2%, indicating better liver function. In terms of immunity, blood lymphocytes were increased in broilers fed a diet supplemented with liquid CLV (17.9 x 103/µl) compared with birds supplemented with dry CLV (13.5 x 103/µl). Additionally, the levels of IgA, IgG, and IgM were elevated by 29.7%, 69.1%, and 32.3%, respectively, in broilers that consumed feed containing CLV. Similarly, the intestinal diversity and abundance of Lactobacillus spp. were significantly increased (9.9 ± 1.88 and 8.99 log10 CFU/g, respectively) by dietary supplementation with liquid CLV compared to that in non-treated chicks (8.7 ± 1.22 and 8.51 log10 CFU/g, respectively). Energy digestibility was increased significantly by 1.29% in CLV-treated chicks compared to the control chicks. This review highlights the findings associated with the utilisation of CLV biomass as a feed supplement and its effect on broiler growth and health.

Keywords

Chlorella vulgarismicroalgaebroilerfeedgrowthmeat qualityimmunity
Type
Review
Information
World's Poultry Science Journal , Volume 75 , Issue 2 , June 2019 , pp. 305 - 318
Copyright
Copyright © World's Poultry Science Association 2019 

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References

AN, B.K., KIM, K.E., JEON, J.Y. and LEE, K.W. (2016) Effect of dried CLV vulgaris and CLV growth factor on growth performance, meat qualities and humoral immune responses in broiler chickens. Springer Plus 5: 718.Google Scholar
AN, H.J., RIM, H.K., JEONG, H.J., HONG, S.H., UM, J.Y. and KIM, H.M. (2010) Hot water extracts of CLV vulgaris improve immune function in proteindeficient weanling mice and immune cells. Immunopharmacol Immunotoxicol 32: 585-592.Google Scholar
AN, H.J., RIM, H.K., LEE, J.H., SEO, M.J. and HONG, J.W. (2008) Effect of CLV vulgaris on immuneenhancement and cytokine production in vivo and in vitro. Food Science and Biotechnology 17: 953-958.Google Scholar
ANDRADE, L.M., ANDRADE, C.J., DIAS, M., NASCIMENTO, C.A.O. and MENDES, M.A. (2018) CLV and Spirulina Microalgae as Sources of Functional Foods, Nutraceuticals, and Food Supplements; an Overview. Food Processing and Technology 6 (1): 00144.Google Scholar
BALTAZAR, M.T., DINIS-OLIVEIRA, R.J., MARTINS, A., BASTOS MDE, L., DUARTE, J.A., GUILHERMINO, L. and CARVALHO, F. (2014) Lysine acetylsalicylate increases the safety of a paraquat formulation to freshwater primary producers: A case study with the microalga CLV vulgaris. Aquatic Toxicology 146: 137-143.Google Scholar
BECKER, E.W. (2004) The nutritional value of microalgae for aquaculture, in: RICHMOND, A. (Ed) Handbook of microalgal mass cultures, pp. 380-391 (CRC Press Inc. Boca Raton, Florida).Google Scholar
BERRI, C., BESNARD, J. and RELANDEAU, C. (2008) Increasing dietary lysine increases final pH and decreases drip loss of broiler breast meat. Poultry Science 87: 480-484.Google Scholar
CHO, J.H. and KIM, I.H. (2014) Effects of lactulose supplementation on performance, blood profiles, excreta microbial shedding of Lactobacillus and Escherichia coli, relative organ weight and excreta noxious gas contents in broilers. Journal of Animal Physiology and Animal Nutrition (Berlin) 98: 424-430.Google Scholar
CHOI, H., JUNG, S.K., KIM, J.S., KIM, K.W., OH, K.B., LEE, P.Y. and BYUN, S.J. (2017) Effects of dietary recombinant CLV supplementation on growth performance, meat quality, blood characteristics, excreta microflora, and nutrient digestibility in broilers. Poultry Science 96 (3): 710-716.Google Scholar
COMBS, G.F. (1952) Algae (CLV) as a source of nutrients for the chick. Science 116: 453-454.Google Scholar
DIAZ, G.J., ROLDÁN, L.P. and CORTÉS, A. (2003) Intoxication of Crotalaria pallida seeds to growing broiler chicks. Veterinary and Human Toxicology 45: 187-189.Google Scholar
DLOUHA, G., SEVCIKOVA, S., DOKOUPILOVA, A., ZITA, L., HEINDL, J. and SKRIVAN, M. (2008) Effect of dietary selenium sources on growth performance, breast muscle selenium, glutathione peroxidase activity and oxidative stability in broilers. Czech Journal of Animal Science 53: 265-269.Google Scholar
DOUCHA, J. (1998) The CLV Programme in the Czech Republic. Inst Microbiology Academic Science of the Czech Republic, Třeboň, 16.Google Scholar
DOUCHA, J. and LÍVANSKÝ, K. (2008) Influence of processing parameters on disintegration of CLV cells in various types of homogenisers. Applied Microbiology Biotechnology 81: 431-440.Google Scholar
DOUCHA, J. and LÍVANSKÝ, K. (2014) High density microalgal culture, in: BAJPAL, R., PROKOP, A. & ZAPPI, M. (Eds) Algal biorefineries, vol 1, pp. 147-171 (Springer Science, Dordrecht).Google Scholar
GRAU, C.R. and KLEIN, N.W. (1957) Sewage-grown algae as a feedstuff for chicks. Poultry Science 36: 1046-1051.Google Scholar
HIDAKA, S., OKAMOTO, Y. and ARITA, M. (2004) A hot water extract of CLV pyrenoidosa reduces body weight and serum lipids in ovariectomized rats. Phytotherapy Research 18: 164-168.Google Scholar
IWAMOTO, H. (2004) Industrial production of microalgal cell-mass and secondary products major industrial species CLV, in: RICHMOND, A. (Ed) Handbook of microalgal mass cultures, pp. 255-263 (CRC Press Inc, Boca Raton).Google Scholar
JANCZYK, P., HALLE, B. and SOUFFRANT, W.B. (2009) Microbial community composition of the crop and ceca contents of laying hens fed diets supplemented with CLV vulgaris. Poultry Science 88: 2324-2332.Google Scholar
JENSEN, G.S., GINSBERG, D.I. and DRAPEAU, C. (2001) Blue-green algae as an immuno-enhancer and biomodulator. Journal of American Nutraceutical Association 3: 24-30.Google Scholar
KANG, H.K. and KIM, C.H. (2016) Effects of dietary supplementation with rice bran oil on the growth performance, blood parameters, and immune response o broiler chickens. Journal of Animal Science and Technology 58: 12.Google Scholar
KANG, H.K., SALIM, H.M., AKTER, N., KIM, D.W., KIM, J.H., BANG, H.T., KIM, M.J., NA, J.C., HWANGBO, J., CHOI, H.C. and SUH, O.S. (2013) Effects of various forms of dietary CLV supplementation on growth performance, immune characteristics, and intestinal microflora population of broiler chickens. Journal of Applied Poultry Science 22: 100-108.Google Scholar
KANOUCHI, H., OKABE, M., DOI, S., YAMADA, H., TACHIBANA, H. and YAMADA, K. (2001) Dietary effect of CLV pyrenoidosa powder on immunoglobulin productivity of Sprague-Dawley rats. Journal of the Japanese Society for Food Science and Technology 48: 634-636.Google Scholar
KEIJIRO, U. (2011) Method for producing CLV fermented food. United States Patent. Patent No.: US 7,914,832 B2.Google Scholar
KOTRBÁČEK, V., DOUBEK, J. and DOUCHA, J. (2015) The chlorococcalean alga CLV in animal nutrition: A review. Journal of Applied Phycology 27: 2173-2180.Google Scholar
KOTRBÁČEK, V., HALOUZKA, R., JURAJDA, V., KNOTKOVÁ, Z. and FILKA, J. (1994) Enhancement of defence mechanisms in broilers after administration of biological feed supplements. Veterinarni Medicina 39: 321-328.Google Scholar
KOTRBÁČEK, V., SKRIVAN, M., KOPECKY, J., PENKAVA, O., HUDEČKOVÁ, P., UHRÍKOVÁ, I. and DOUBEK, J. (2013) Retention of carotenoids in egg yolks of laying hens supplemented with heterotrophic CLV. Czech Journal of Animal Science 58: 193-200.Google Scholar
LI, H.B., JIANG, Y. and CHEN, F. (2002) Isolation and purification of lutein from the microalga CLV vulgaris by extraction after saponification. Journal of Agricultural and Food Chemistry 50 (5): 1070-1072.Google Scholar
LIPSTEIN, B. and HURWITZ, S. (1981) The nutritional value of sewage-grown, alum-flocculated Micractinium algae in broiler and layer diets. Poultry Science 60: 2628-2638.Google Scholar
MASON, R. (2001) CLV and Spirulina: Green supplements for balancing the body. Alternative and Complementary Therapies 7 (3): 161-165.Google Scholar
NAKANO, S., TAKEKOSHI, H. and NAKANO, M. (2007) CLV (CLV pyrenoidosa) supplementation decreases dioxin and increases immunoglobulin A concentrations in breast milk. Journal of Medicinal Food 10: 134-142.Google Scholar
OH, S.T., ZHENG, L., KWON, H.J., CHOO, Y.K., LEE, K.W., KANG, C.W. and AN, B.K. (2015) Effects of dietary fermented CLV vulgaris (CBT®) on growth performance, relative organ weights, cecal microflora, tibia bone characteristics, and meat qualities in Pekin ducks. Asian-Australasian Journal of Animal Sciences 28: 95-101.Google Scholar
PEIRETTI, P.G. and MEINERI, G. (2008) Effects of diets with increasing levels of Spirulina platensis on the performance and apparent digestibility in growing rabbits. Livestock Science 118: 173-177.Google Scholar
PUGH, N., ROSS, S.A., ELSOHLY, H.N., ELSOHLY, M.A. and PASCO, D.S. (2001) Isolation of three high molecular weight polysaccharide preparations with potent immunostimulatory activity from Spirulina platensis, Aphanizomenon flos-aquae and CLV pyrenoidosa. Planta Medica 67: 737-742.Google Scholar
QURESHI, M.A., GARLICH, J.D. and KIDD, M.T. (1996) Dietary Spirulina platensis enhances humoral and cell-mediated immune functions in chickens. Immunopharmacology and Immunotoxicology 18: 465-476Google Scholar
RYU, N.H., LIM, Y., PARK, J.E., KIM, J.H., KIM, J.Y., KWON, S.W. and KWON, O. (2014) Impact of daily CLV consumption on serum lipid and carotenoid profiles in mildly hypercho lesterolemic adults: a doubleblinded, randomized, placebocontrolled study. Nutrition Journal 13: 57-64.Google Scholar
ŠEVČÍKOVÁ, S., SKŘIVAN, M., DLOUHÁ, G. and KOUCKÝ, M. (2006) The effect of selenium source on the performance and meat quality of broiler chickens. Czech Journal of Animal Science 51: 449-457.Google Scholar
SKŘIVAN, M., ŠIMÁNĚ, J., DLOUHÁ, G. and ŠEVČÍKOVÁ, S. (2008) Dietary selenium increases vitamin E contents of egg yolk and chicken meat. British Poultry Science 49: 482-486.Google Scholar
TAKEKOSHI, H., SUZUKI, G. and CHUBACHI, H. (2005) Effect of CLV pyrenoidosa on fecal excretion and liver accumulation of polychlorinated dibenzo-p-dioxin in mice. Chemosphere 59: 297-304.Google Scholar
WANG, J.P., ZHANG, Z.F., YAN, L. and KIM, I.H. (2016) Effects of dietary supplementation of emulsifier and carbohydrase on the growth performance, serum cholesterol and breast meat fatty acids profile of broiler chickens. Animal Science Journal 87: 250-256.Google Scholar
YAMADA, K., TOKUNAGA, Y., IKEDA, A., OHKURA, K., KAKU-OHKURA, S., MAMIYA, S., LIM, B.O. and TACHIBANA, H. (2003) Effect of dietary fiber on the lipid metabolism and immune function of aged Sprague-Dawley rats. Bioscience, Biotechnology and Biochemistry 67: 429-433.Google Scholar
ZHENG, L., OH, S.T., JEON, J.Y., MOON, B.H., KWON, H.S., LIM, S.U., AN, B.K. and KANG, C.W. (2012) The dietary effects of fermented CLV vulgaris (CBT) on production performance, liver lipids and intestinal microflora in laying hens. Asian-Australian Journal of Animal Science 25: 261-266.Google Scholar