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Sex identification in avian species using DNA typing methods

Published online by Cambridge University Press:  14 March 2007

H. CERIT  and
K. AVANUS
H. CERIT*
Affiliation:
Istanbul University Veterinary Faculty Department of Animal Breeding and Genetics, Istanbul, Turkey
K. AVANUS
Affiliation:
Istanbul University Veterinary Faculty Department of Animal Breeding and Genetics, Istanbul, Turkey
*
*Corresponding author: hcerit@istanbul.edu.tr
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Abstract

Many avian species are considered sexually monomorphic. In monomorphic bird species, especially in young birds, sex is difficult to identify based on an analysis of their external morphology. For nestlings, sex identification is usually impossible by morphological examination. Sex identification in avian species is one of the key points of avian breeding and evolutionary studies. Through the knowledge of sex identification genes, poultry breeding programmes can be applied more successfully. Vent sexing, laparoscopy, steroid sexing and karyotyping are methods for sex identification used in monomorphic birds. However these methods are unreliable, time-consuming, and expensive while some of them could be painful and even lifethreatening for birds. The sex of an individual is established from the genes located on sex chromosomes. Female birds carry one copy of both Z and W, and male birds carry two copies of Z avian sex chromosomes. However, there are many DNA techniques, the most reliable one is amplifying sex specific CHD1 gene by P2 – P8 primer pairs.

Keywords

sex identificationavian speciesavian sex chromosomes, CHDZ geneCHDW gene
Type
Review Article
Information
World's Poultry Science Journal , Volume 63 , Issue 1 , March 2007 , pp. 91 - 100
Copyright
Copyright © World's Poultry Science Association 2007

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References

ALBERTS, B., JOHNSON, A., LEWIS, J., RAFF, M., ROBERTS, K. and WALTER, P. (2002) Manipulating proteins, DNA, and RNAIn: Molecular Biology of the Cell; Garland Science, New York, 4th Edition, pp: 508–509.Google Scholar
ARCHAWARANON, M. (2004) Rapid sexing hill mynah Gracula religiosa by sex chromosomes. Biotechnology 3: 160–164.Google Scholar
BIRKHEAD, T.R., HATCHWELL, B.J. and LINDNER, R. (2001) Extra-pair paternity in the common murre. The Cooper Ornithological Society 103: 158–162.Google Scholar
BRAMWELL, R.K. (2003) Sexing chicks in the backyard flock. Avian Advice 5: 4–5.Google Scholar
BOUTETTE, J.B., RAMSAY, E.C., POTGIETER, L.N.D. and KANIA, S.A. (2002) An improved polymerase chain reaction–restriction fragment length polymorphism assay for gender identification in birds. Journal of Avian Medicine and Surgery 16: 198–202.Google Scholar
CANON, N.R., TELL, L.A., NEEDHAM, M.L. and GARDNER, A.A. (2000) Flow cytometric analysis of nuclear DNA for sex identification in three psittacine species. American Journal of Veterinary Research 61: 847–850.Google Scholar
CERIT, H. and AVANUS, K. (2006a) Sex identification of parrots from feather DNA using CHDW and CHDZ genes of avian sex chromosomes. Turkish Journal of Veterinary and Animal Sciences. In press.Google Scholar
CERIT, H. and AVANUS, K. (2006b) Sex identification by CHDW and CHDZ genes of avian sex chromosomes in Nymphicus hollandicus. World's Poultry Science Journal Supplement, XII European Poultry Conference 10–14 September 62: 216.Google Scholar
CHENG, Y.H., KUO, T.F., LEE, D.N. and WENG, C.F. (2006) Sex identification of the black-faced spoonbill (Platalea minor). Zoological Studies 45: 104–113.Google Scholar
CHRISTIDIS, L. (1985) A rapid procedure for obtaining chromosome preparations from birds. Auk 102: 892–893.Google Scholar
CLINTON, M. (1994) A rapid protocol for sexing chick embryos. Animal Genetics 25: 361–362.CrossRefGoogle ScholarPubMed
CANON, N.R., TELL, L.A., NEEDHAM, M.L. and GARDNER, A.A. (2000) Flow cytometric analysis of nuclear DNA for sex identification in three psittacine species. American Journal of Veterinary Research 61: 847–850.CrossRefGoogle ScholarPubMed
DAWSON, D.A., DARBY, S., HUNTER, F.M., KRUPA, A.P., JONES, I.L. and BURKE, T. (2001) A critique of avian CHD-based molecular sexing protocols illustrated by a Z-chromosome polymorphism detected in auklets. Molecular Ecology Notes 1: 201–204.CrossRefGoogle Scholar
ELLEGREN, H. (1991) DNA typing of museum birds. Nature 354: 113.CrossRefGoogle ScholarPubMed
ELLEGREN, H. (2001) Hens, cocks and avian sex identification. Aquest for genes on Z or W? EMBO Reports 2: 192–196.Google Scholar
ELLEGREN, H. and SHELDON, B.C. (1997) New tools for sex identification and the study of sex allocation in birds. Trends in Ecology and Evolution 12: 255–259.CrossRefGoogle Scholar
FRIDOLFSSON, A.K., CHENG, H., COPELAND, N.G., JENKINS, N.A. and LIU, H. (1998) Evolution of the avian sex chromosomes from an ancestral pair of autosomes. Proceedings of the National Academy of Sciences 95: 8147–8152.CrossRefGoogle ScholarPubMed
FRIDOLFSSON, A.K. and ELLEGREN, H. (1999) Molecular evolution of the avian CHD1 genes on the Z and W sex chromosomes. Genetics 155: 1903–1912.CrossRefGoogle Scholar
GARCIA-MORENO, J. and MINDELL, D.P. (2000) Rooting a phylogeny with homologous genes on opposite sex chromosomes (gametologs): A case study using avian CHD. Molecular Biology Evolution 17: 1826–1832.CrossRefGoogle ScholarPubMed
GRAVES, J., ORTEGA-RUANO, J. and SLATER, P.J.B. (1993) Sex ratio of chicks in the Shag Phalacrocorax aristotelis determined by a female-specific band in DNA fingerprinting. Ibis 135: 470–472.CrossRefGoogle Scholar
GRIFFITHS, R., DOUBLE, M.C., ORR, K. and DAWSON, R.J. (1998) A DNA test to sex most birds. Molecular Ecology 7: 1071–1075.Google Scholar
GRIFFITHS, R. and KORN, R.M. (1997) A CHD1 gene is Z chromosome linked in the chicken Gallus domesticus. Gene 197: 225–229.CrossRefGoogle ScholarPubMed
GRIFFITHS, R. and ORR, K. (1999) The use of amplified fragment length polymorphism (AFLP) in the isolation of sex-specific markers. Molecular Ecology 8: 671–674.Google Scholar
GRIFFITHS, R. and TIWARI, B. (1993) The isolation of molecular genetic markers for the identification of sex. Proceedings of the National Academy of Sciences 90: 8324–8326.Google Scholar
GRIFFITHS, R. and TIWARI, B. (1995) Sex of the last wild Spix's macaw. Nature 375: 454.Google ScholarPubMed
HONKATUKIA, M., KULMALA, J. and SÖDERBACK, P. (2003) Isolation of genomic DNA from chicken feathers using QuickPickTM gDNA kit. Technical Note TN51000–009. Bio-Nobile Oy pp 1–2.Google Scholar
JENSEN, T., PERNASETTI, F.M. and DURRANT, B. (2003) Conditions for rapid sex determination in 47 avian species by PCR of genomic DNA from blood, shell-membrane blood vessels and feathers. Zoo Biology 22: 561–567.CrossRefGoogle Scholar
KAHN, N.W., JOHN, J. and QUINN, T. (1998) Chromosome-specific intron size differences in the Avian CHD gene provide an efficient method for sex identification in birds. The Auk 115: 1074–1078.Google Scholar
KLUG, W.S. and CUMMMINGS, M.R. (2000) Construction and analyses of DNA clones. In: Concepts of genetics Eds:, Prentice Hall Inc.: New Jersey, pp: 515–517.Google Scholar
LESSELLS, C. and MATEMAN, A. (1998) Sexing birds using random amplified polymorphic DNA (RAPD) markers. Molecular Ecology 7: 187–195.CrossRefGoogle Scholar
MALAGÓ, W. Jr, HEITOR, M.F., MATHEUCCI, E. Jr, MEDAGLIA, A. and HENRIQUE-SILVA, F. (2002) Large scale sex typing of ostriches using DNA extracted from feathers. BMC biotechnology 2: 19 (http://biomedcentral.com/1472-6750/2/19).CrossRefGoogle ScholarPubMed
MILLAR, C.D., LAMBERT, D.M., BELLAMY, A.R., STAPLETON, P.M. and YOUNG, E.C. (1992) Sexspecific restriction fragments and sex ratios revealed by DNA fingerprinting in the brown skua. The American Genetic Association 83: 350–355.Google Scholar
MILLAR, C.D., LAMBERT, D.M., ANDERSON, S. and HALVERSON, J.L. (1996) Molecular sexing of communally breeding pukeko: An important ecological tool. Molecular Ecology 5: 289.CrossRefGoogle ScholarPubMed
MILLS, L.S., CITTA, J.J., LAIR, K.P., SCHWARTZ, M.K. and TALLMON, D.A. (2000) Estimating animal abundance using noninvasive DNA sampling: Promise and pittfalls. Ecological Applications 10: 283–294.CrossRefGoogle Scholar
MIYAKI, C.Y., DUARTE, M.B., CAPARROZ, R., NUNES, A.V. and WAJNTAL, A. (1997) Sex identification of South American Parrots (Psittacidae, Aves) using the human minisatellite probe 33.15. The Auk 114: 516–520.CrossRefGoogle Scholar
NESJE, M. and RØED, K. (2000) Sex identification in falcons using microsatellite DNA markers. Hereditas 132: 261–263.CrossRefGoogle ScholarPubMed
OGAWA, A., SOLOVEI, I., HUTCHISON, N., SAITOH, Y., IKEDA, J.E., MACGREGOR, H. and MIZUNO, S. (1997) Molecular characterization and cytological mapping of a non-repetitive DNA sequence region from the W chromosome of chicken and its use as a universal probe for sexing Carinitae birds. Chromosome Research 5: 93–101.Google Scholar
QUINN, T.W., COOKE, F. and BRADLEY, N.W. (1990) Molecular sexing of geese using a cloned Z chromosomal sequence with homology to the Wchromosome. Auk 107: 199–202.Google Scholar
PETITE, J.N. and KEGELMEYER, A.E. (1992) Sex Determination of chick embryos using a W chromosome-specific oligonucleotide probe and PCR. Proceedings of the XIX World's Poultry Congress Amsterdam 531.Google Scholar
PETRIE, M., SCHWABL, H., BRANDE-LAVRIDSEN, N. and BURKE, T. (2001) Sex differences in avian yolk hormone levels. Nature 412: 498.CrossRefGoogle ScholarPubMed
RUSSEL, P.J. (2002) iGenetics. Eds: Benjamin Cummings, CA. pp 190–192, 223–224, 644–645.Google Scholar
SAITOH, Y., SAITOH, H., OHTOMO, K. and MIZUNO, S. (1991) Occupancy of the majority of DNA in the chicken W-chromosome by bent-repetitive sequences. Chromosoma 101: 32–40.CrossRefGoogle ScholarPubMed
SHEPHARD, J.M., CATTERALL, C.P. and HUGHES, J.M. (2004) Discrimination of sex in the whitebellied sea-eagle, Haliaeetus leucogaster, using genetic and morphometric techniques. Emu 104: 83–87.CrossRefGoogle Scholar
SOUTH, J.M. and WRIGHT, T.M. (2002) Nestling sex ratios in the yellow-naped amazon: no evidence for adaptive modification. The Condor 104: 437–440.Google Scholar
SWENGEL, S.R. (1996) Special techniques, C: Sex determinationIn: Cranes: Their Biology, Husbandry, and Conservation; Ellis, D.H.; Gee, G.F.; Mirande, C.M. Eds.; National Biological Service/International Crane Foundation: United States of America, pp223–231.Google Scholar
WELSH, J. and MC CLEALLAND, M. (1990) Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acid Research 18: 7213–7218.CrossRefGoogle ScholarPubMed
WILLIAMS, J.K.G., RUBELIK, A.R., LIVAK, K.J., RAFALSKI, J.A. and TINGLEY, S.V. (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Research 18: 6531–6535.Google Scholar
WINK, M., SAUER-GURTH, H., MARTINEZ, F., DOVAL, G., BLANCO, G. and HATZOVE, O. (1998) The use of (GACA)4 PCR to sex old world vultures (Aves: Accipitridae). Molecular Ecology 7: 779–782.CrossRefGoogle Scholar
Von ENGELHARDT, N. and GROOTHIUS, T.G.G. (2005) Measuring steroid hormones in avian eggs. Annals of the New York Academy of Sciences 1046: 181–192.CrossRefGoogle ScholarPubMed