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Aldehyde dehydrogenase in fresh primordial germ cells as a marker of cell ‘stemness’

Published online by Cambridge University Press:  01 February 2019

Andrea Svoradová Open the ORCID record for Andrea Svoradová [Opens in a new window] ,
Jaromír Vašíček ,
Alexander Ostró  and
Peter Chrenek
Andrea Svoradová*
Affiliation:
Constantine the Philosopher University in Nitra, Faculty of Natural Sciences, Department of Zoology and Anthropology, Tr. A. Hlinku 1, 949 74 Nitra, Slovak Republic
Jaromír Vašíček
Affiliation:
Research Institute for Animal Production in Nitra, NPPC, Lužianky, Slovak Republic Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Science, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic
Alexander Ostró
Affiliation:
Pavol Jozef Šafárik University in Košice, Faculty of Medicine, Trieda SNP 1, 040 11, Košice, Slovak Republic
Peter Chrenek
Affiliation:
Research Institute for Animal Production in Nitra, NPPC, Lužianky, Slovak Republic Slovak University of Agriculture in Nitra, Faculty of Biotechnology and Food Science, Tr. A. Hlinku 2, 949 76 Nitra, Slovak Republic University of Science and Technology, Department of Animal Biochemistry and Biotechnology, Al. prof. S. Kaliskiego 7, 85-796, Bydgoszcz, Poland
*
*Address for correspondence: A. Svoradová et al. (2018) Constantine the Philosopher University in Nitra, Faculty of Natural Sciences, Department of Zoology and Anthropology, Tr. A. Hlinku 1, 949 74 Nitra, Slovak Republic. Tel: +421 37 640 8720. E-mail: andrea.svoradova@ukf.sk
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Summary

Chicken primordial germ cells (PGCs) are the primary pluripotent stem cell types that will differentiate towards germ cells. High aldehyde dehydrogenase (ALDH) activity is considered as a functional marker for the detection of cell ‘stemness’. In our study the ALDEFLUOR™ kit was used for determination of ALDH activity in PGCs. PGCs were co-stained with diethylaminobenzaldehyde (DEAB) and ALDH and analyzed by flow cytometry. Our results showed a small cell population (8.0 ± 3.3%) upon preincubation of the cells with the specific inhibitor DEAB, however cells without inhibitor staining showed a fluorescence shift as an ALDH-positive population (70.5 ± 1.6%). These findings indicate higher expression of ALDH in PGCs and ALDH activity can therefore be used as a new functional marker for the detection of cell ‘stemness’ in chicken PGCs. These results may have importance for characterization of PGCs as a potential genetic resource in poultry. Further research is necessary to elucidate the role of this functional marker in these cells.

Keywords

ALDEFLUORALDHChickenFlow cytometryPrimordial germ cells
Type
Short Communication
Information
Zygote , Volume 27 , Issue 1 , February 2019 , pp. 46 - 48
Copyright
© Cambridge University Press 2019 

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