Hostname: page-component-cd9895bd7-8ctnn Total loading time: 0 Render date: 2024-12-26T06:09:34.105Z Has data issue: false hasContentIssue false
  • English
  • Français

Assessment of binder of sperm protein 1 (BSP1) and heparin effects on in vitro capacitation and fertilization of bovine ejaculated and epididymal sperm

Published online by Cambridge University Press:  10 August 2020

Paula Rodríguez-Villamil ,
Daiane Mentz ,
Felipe Ledur Ongaratto Open the ORCID record for Felipe Ledur Ongaratto [Opens in a new window] ,
Luis Henrique Aguiar ,
Jose Luiz Rodrigues ,
Marcelo Bertolini  and
Arlindo A. Moura Open the ORCID record for Arlindo A. Moura [Opens in a new window]
Paula Rodríguez-Villamil
Affiliation:
Animal Physiology Laboratory, Federal University of Ceará, Fortaleza, Brazil Embryology and Reproduction Biotechnology Laboratory, Federal University of Rio Grande do Sul, Porto Alegre, Brazil
Daiane Mentz
Affiliation:
Embryology and Reproduction Biotechnology Laboratory, Federal University of Rio Grande do Sul, Porto Alegre, Brazil
Felipe Ledur Ongaratto
Affiliation:
Embryology and Reproduction Biotechnology Laboratory, Federal University of Rio Grande do Sul, Porto Alegre, Brazil
Luis Henrique Aguiar
Affiliation:
Embryology and Reproduction Biotechnology Laboratory, Federal University of Rio Grande do Sul, Porto Alegre, Brazil
Jose Luiz Rodrigues
Affiliation:
Embryology and Reproduction Biotechnology Laboratory, Federal University of Rio Grande do Sul, Porto Alegre, Brazil
Marcelo Bertolini
Affiliation:
Embryology and Reproduction Biotechnology Laboratory, Federal University of Rio Grande do Sul, Porto Alegre, Brazil
Arlindo A. Moura*
Affiliation:
Animal Physiology Laboratory, Federal University of Ceará, Fortaleza, Brazil
*
Author for correspondence: Arlindo A. Moura. Department of Animal Science. Av. Mister Hull, s/n Campus do Pici. FortalezaCE60440-554, Brazil. Tel: +55 85 3366 9697. E-mail: arlindo.moura@gmail.com
Get access Rights & Permissions [Opens in a new window]

Summary

The present study evaluated the effect of binder of sperm protein 1 (BSP1) and/or heparin on in vitro bovine capacitation and fertilization rates using epididymal and ejaculated bovine sperm. Frozen–thawed sperm were selected and used in the following treatments. Control group: Fert-TALP medium without heparin; heparin (HEP) group: Fert-TALP with heparin (10 UI/ml); BSP1 group: Fert-TALP medium with BSP1 (10 µg/ml for ejaculated sperm; 40 µg/ml for epididymal sperm); HEP + BSP1 group: Fert-TALP medium with heparin (5 UI/ml) and BSP1 (5 µg/ml for ejaculated sperm; 20 µg/ml for epididymal sperm) and determined in vitro capacitation rates in different interval times (0, 15, 30 and 60 min) using the chlortetracycline fluorescence (CTC) method. Also, we evaluated the development rates of oocytes fertilized with ejaculated or epididymal sperm into the same treatments. Capacitation was greater and faster when ejaculated sperm were treated for 60 min with heparin compared with other treatments. However, developmental rates were similar in all treatments. For epididymal sperm, the treatments with BSP1 presented higher capacitation and fertilization rates compared with heparin (P < 0.05). The effects of heparin + BSP1 on capacitation and developmental rates did not cause any increase in capacitation or blastocyst rates compared with other groups for ejaculated or epididymal sperm. In conclusion, this study confirmed that either BSP1 and heparin can be used as capacitator agents for bovine ejaculated sperm during IVF. However, BSP1 seems to be more efficient compared with heparin for epididymal sperm. Furthermore, BSP1 and heparin have no synergic effects on sperm capacitation.

Keywords

BSP1HeparinIVFSperm capacitationSperm protein
Type
Research Article
Information
Zygote , Volume 28 , Issue 6 , December 2020 , pp. 489 - 494
Copyright
© The Author(s), 2020. Published by Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ax, RL and Lenz, RW (1987). Glycosaminoglycans as probes to monitor differences in fertility of bulls. J Dairy Sci 70, 1477–86.CrossRefGoogle ScholarPubMed
Córdoba, M, Santa-Coloma, TA, Beorlegui, NB and Beconi, MT (1997). Intracellular calcium variation in heparin-capacitated bovine sperm. Biochem Mol Biol Int 41, 725–33.Google ScholarPubMed
Fraser, LR (1995). Ionic control of sperm function. Reprod Fertil Dev 784, 905–25.CrossRefGoogle Scholar
Gadella, BM (2008). The assembly of a zona pellucida binding protein complex in sperm. Reprod Domest Anim 5, 12–9.CrossRefGoogle Scholar
Gadella, BM and Luna, C (2014). Cell biology and functional dynamics of the mammalian sperm surface. Theriogenology 81, 74–84 CrossRefGoogle ScholarPubMed
Harrison, RA and Gadella, BM (2005). Bicarbonate-induced membrane processing in sperm capacitation. Theriogenology 63, 342–51.CrossRefGoogle ScholarPubMed
Liberda, J, Ryslavá, H, Jelínková, P, Jonáková, V and Tichá, M (2002). Affinity chromatography of bull seminal proteins on mannan-Sepharose. J Chromatogr B 25, 231–9.CrossRefGoogle Scholar
Manjunath, P and Thérien, I (2002). Role of seminal plasma phospholipid-binding proteins in sperm membrane lipid modification that occurs during capacitation. J Reprod Immunol 53, 109–19.CrossRefGoogle ScholarPubMed
Manjunath, P, Sairam, MR and Uma, J (1987). Purification of four gelatin-binding proteins from bovine seminal plasma by affinity chromatography. Biosci Rep 7, 231–8.CrossRefGoogle ScholarPubMed
Manjunath, P, Bergeron, A, Lefebvre, J and Fan, J (2007). Seminal plasma proteins: functions and interaction with protective agents during semen preservation. Soc Reprod Fertil Suppl 65, 217–28.Google ScholarPubMed
Manjunath, P, Lefebvre, J, Jois, PS, Fan, J and Wright, MW (2009). New nomenclature for mammalian BSP genes. Biol Reprod 80, 394–7.CrossRefGoogle ScholarPubMed
Parrish, JJ (2014). Bovine in vitro fertilization: in vitro oocyte maturation and sperm capacitation with heparin. Theriogenology 81, 67–73.CrossRefGoogle ScholarPubMed
Parrish, JJ, Susko-Parrish, JL, Handrow, R, Sims, MM and First, NL (1989). Capacitation of bovine spermatozoa by oviduct fluid. Biol Reprod 40, 1020–5.CrossRefGoogle ScholarPubMed
Parrish, JJ, Krogenaes, A and Susko-Parrish, JL (1995). Effect of bovine sperm separation by either swim-up or Percoll method on success of in vitro fertilization and early embryonic development. Theriogenology 44, 859–69.CrossRefGoogle ScholarPubMed
Parrish, JJ, Susko-Parrish, J, Winer, MA and First, NL (1998). Capacitation of bovine sperm by heparin. Biol Reprod 38, 1171–80.CrossRefGoogle Scholar
Plante, G, Thérien, I and Manjunath, P (2012). Characterization of recombinant murine binder of sperm protein homolog 1 and its role in capacitation. Biol Reprod 87, 1–11.CrossRefGoogle ScholarPubMed
Plante, G, Thérien, I, Lachance, C, Leclerc, P, Fan, J and Manjunath, P (2014). Implication of the human Binder of SPerm Homolog I (BSPHI) protein in capacitation. Mol Hum Reprod 20, 409–21.CrossRefGoogle Scholar
Plante, G, Prud’homme, B, Fan, J, Lafleur, M and Manjunath, P (2016). Evolution and function of mammalian binder of sperm proteins. Cell Tissue Res., 363, 105–27.CrossRefGoogle ScholarPubMed
Rego, JPA, Crisp, JM, Moura, AA, Nouwens, AS, Li, Y; Venus, B, Corbet, NJ, Corbet, DH, Burns, BM, Boe-Hansen, GB and McGowan, MR (2014). Seminal plasma proteome of electroejaculated Bos indicus bulls. Anim Reprod Sci 148, 1–17.CrossRefGoogle ScholarPubMed
Robertson, I and Nelson, R (2007). Manual of the International Embryo Transfer Society : Certification and identification of the embryo. USA: Savory, Hill: International Embryo Transfer Society.Google Scholar
Rodriguez-Martinez, H, Kvist, U, Ernerudh, J, Sanz, L and Calvete, JJ (2011). Seminal plasma proteins: what role do they play? Am J Reprod Immunol 66, 11–22.CrossRefGoogle ScholarPubMed
Rodríguez-Villamil, P, Wei, H, Moreira, G, Caccia, M, Fernandez Taranco, M and Bó, GA (2012). Fertilization rates and in vitro embryo production using sexed or non-sexed semen selected with a silane-coated silica colloid or Percoll. Theriogenology 78, 165–71.CrossRefGoogle ScholarPubMed
Rodríguez-Villamil, P, Marulanda, VH, Martins, JAM, Oliveira, AN and Aguiar, LH (2016). Purification of binder of sperm protein 1 (BSP1) and its effects on bovine in vitro embryo development after fertilization with ejaculated and epididymal sperm. Theriogenology 85, 540–54.CrossRefGoogle ScholarPubMed
Sankhala, RS, Damai, RS, Anbazhagan, V, Kumar, CS, Bulusu, G and Swamy, MJ (2011). Biophysical investigations on the interaction of the major bovine seminal plasma protein, PDC-109, with heparin. J Phys Chem B 115, 12954–62.CrossRefGoogle ScholarPubMed
Souza, CE, Moura, AA, Monaco, E and Killian, GJ (2008). Binding patterns of bovine seminal plasma proteins A1/A2, 30 kDa and osteopontin on ejaculated sperm before and after incubation with isthmic and ampullary oviductal fluid. Anim Reprod Sci 105, 72–89.CrossRefGoogle ScholarPubMed
Thérien, I, Bleau, G and Manjunath, P (1995). Phosphatidylcholine-binding proteins of bovine seminal plasma modulate capacitation of spermatozoa by heparin. Biol Reprod 52, 1372–9.CrossRefGoogle ScholarPubMed
Thérien, I, Moreau, R and Manjunath, P (1999). Bovine seminal plasma phospholipid-binding proteins stimulate phospholipid efflux from epididymal sperm. Biol Reprod 61, 590–8.CrossRefGoogle ScholarPubMed
Thérien, I, Bergeron, A, Bousquet, D and Manjunath, P (2005). Isolation and characterization of glycosaminoglycans from bovine follicular fluid and their effect on sperm capacitation. Mol Reprod Dev 71, 97–106.CrossRefGoogle ScholarPubMed
Therrien, A, Manjunath, P and Lafleur, M (2013). Chemical and physical requirements for lipid extraction by bovine binder of sperm BSP1. Biochim Biophys Acta Biomemb 2, 543–51.CrossRefGoogle Scholar
Turmo, D, Mondéjar, I, Grullón, L, Calvete, J, Manjunath, P, Coy, P and Aviles, M (2009). Binder of sperm 1 is involved in the sperm binding to the zona pellucida in the bovine species. Presented at the XIII National Meeting of the Spanish Cell Biology Society (SEBC). Murcia, Spain p. 225.Google Scholar
Visconti, PE and Kopf, GS (1998). Regulation of protein phosphorylation during sperm capacitation. Biol Reprod 59, 1–6.CrossRefGoogle ScholarPubMed
Visconti, PE, Krapf, D, de la Vega-Beltran, JL, Acevedo, JJ and Darszon, A (2011). Ion channels, phosphorylation and mammalian sperm capacitation. Asian J Androl 13, 395–405.CrossRefGoogle ScholarPubMed
Xia, J and Ren, D (2009). The BSA-induced Ca2+ influx during sperm capacitation is CATSPER channel-dependent. Reprod Biol Endocrinol 7, 119.CrossRefGoogle ScholarPubMed
Yanagimachi, R (1994). The Physiology of Reproduction: Mammalian Fertilization (eds Knobil, E. and Neill, J.D.) New York.Google Scholar