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Assessment of successful pregnancy using granular oocytes in ICSI treatments

Published online by Cambridge University Press:  15 March 2019

P. Tulay Open the ORCID record for P. Tulay [Opens in a new window] ,
H. Arslan ,
A. Buran  and
Y. Koprulu
P. Tulay*
Affiliation:
Near East University, Faculty of Medicine, Department of Medical Genetics, Nicosia North, Cyprus Near East University, Research Center of Experimental Health Sciences (DESAM), Nicosia North, Cyprus
H. Arslan
Affiliation:
Faruk Medical City, Department of Embryology, Suleymaniyah, Iraq
A. Buran
Affiliation:
Elazig Medical Park Hospital, Department of Embryology, Elazig, Turkey
Y. Koprulu
Affiliation:
Faruk Medical City, Department of Urology, Suleymaniyah, Iraq
*
Address for correspondence: Pinar Tulay. Near East University, Faculty of Medicine, Department of Medical Genetics, Near East Boulvard, Nicosia North, Cyprus. E-mail: pinar.tulay@neu.edu.tr
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Summary

Different parameters affect the success of assisted reproduction technology (ART) treatments. One of the advantages of using intracytoplasmic sperm injection (ICSI) is that it also enables the assessment of the oocyte morphology. To date there has not been a clear conclusion on aberrant oocyte morphology and its consequences on the success of ART treatments. Therefore, in this study, we aimed to investigate the fertilization, embryo development and pregnancy rates in patients who have oocytes with granular cytoplasm. Additionally, we investigated if there were more aneuploid embryos obtained from abnormal cytoplasmic morphology. In total, 5704 oocytes were collected and, of these, 4036 were metaphase II (MII) oocytes. The morphology of these oocytes was assessed following denudation and 970 oocytes were observed to have granular cytoplasm. There was no difference in the fertilization rates between the oocytes with normal cytoplasm (89%) and oocytes with granular cytoplasm (72%). Cleavage of embryos and the number of embryos that reached the blastocyst stage were also similar in these two groups. The aneuploidy rates between the two groups were also similar. However, clinical pregnancies were significantly lower in embryos obtained from oocytes with granular cytoplasm (37.5% vs 70%, P<0.05). Therefore, the morphology of the oocyte is as important as morphology of the sperm. Even though normal fertilization and cleavage were achieved from oocytes with granular cytoplasm, their implantation potential was significantly compromised.

Keywords

FertilizationGranular oocytesICSIPregnancy
Type
Research Article
Information
Zygote , Volume 27 , Issue 2 , April 2019 , pp. 97 - 100
Copyright
© Cambridge University Press 2019 

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