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The effect of Xenopus laevis egg extracts with/without BRG1 on the development of preimplantation cloned mouse embryos

Cite this article: Chien-Yueh Chiang et al. (2019) The effect of Xenopus laevis egg extracts with/without BRG1 on the development of preimplantation cloned mouse embryos. Zygote27: 143–152. doi: 10.1017/S0967199419000091

Published online by Cambridge University Press:  11 June 2019

Chien-Yueh Chiang ,
Xin-Yu Chen ,
Chun-Ting Lin  and
Pin-Chi Tang Open the ORCID record for Pin-Chi Tang [Opens in a new window]
Chien-Yueh Chiang
Affiliation:
Department of Animal Science, National Chung Hsing University, Taichung, 402, Taiwan
Xin-Yu Chen
Affiliation:
Department of Animal Science, National Chung Hsing University, Taichung, 402, Taiwan
Chun-Ting Lin
Affiliation:
Department of Animal Science, National Chung Hsing University, Taichung, 402, Taiwan
Pin-Chi Tang*
Affiliation:
Department of Animal Science, National Chung Hsing University, Taichung, 402, Taiwan The iEGG and Animal Biotechnology Center, National Chung Hsing University, Taichung, 4027, Taiwan Center for the Integrative and Evolutionary Galliformes Genomics, National Chung Hsing University, Taichung, 402, Taiwan
*
*Address for correspondence: Pin-Chi Tang. National Chung Hsing University, Taichung, 402, Taiwan. Tel: +886 4 22857109. Fax: +886 4 22860265. E-mail: pctang@dragon.nchu.edu.tw
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Summary

Much effort has been devoted to improving the efficiency of animal cloning. The aim of this study was to investigate the effect of BRG1 contained in Xenopus egg extracts on the development of cloned mouse embryos. The results showed that mouse NIH/3T3 cells were able to express pluripotent genes after treatment with egg extracts, indicating that the egg extracts contained reprogramming factors. After co-injection of Xenopus egg extracts and single mouse cumulus cells into enucleated mouse oocytes, statistically higher pronucleus formation and development rates were observed in the egg Extract− co-injected group compared with those in the no egg extract-injected (NT) group (38–66% vs 18–34%, P<0.001). Removal of BRG1 protein from Xenopus egg extracts was conducted, and the BRG1-depleted extracts were co-injected with single donor cells into recipient oocytes. The results showed that the percentages of pronucleus formation were significantly higher in both BRG1-depleted and BRG1-intact groups than that in the nuclear transfer (NT) group (94, 64% vs 50%, P<0.05). Furthermore, percentages in the BRG1-depleted group were even higher than in the BRG1-intact group (94% vs 64%). More confined expression of Oct4 in the inner cell mass (ICM) was observed in the blastocyst derived from the egg extract-injected groups. However, Nanog expression was more contracted in the ICM of cloned blastocysts in the BRG1-depleted group than in the BGR1-intact group. Based on the present study, BRG1 might not play an essential role in reprogramming, but the factors enhancing pronucleus formation and development of cloned mouse embryos are contained in Xenopus egg extracts.

Keywords

BRG1InjectionReprogrammingSomatic cell nuclear transferXenopus egg extracts
Type
Research Article
Information
Zygote , Volume 27 , Issue 3 , June 2019 , pp. 143 - 152
Copyright
© Cambridge University Press 2019 

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Footnotes

*

These authors contributed equally to this work.

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