Hostname: page-component-78c5997874-m6dg7 Total loading time: 0 Render date: 2024-11-10T15:52:28.556Z Has data issue: false hasContentIssue false
  • English
  • Français

The efficacy of the well of the well (WOW) culture system on development of bovine embryos in a small group and the effect of number of adjacent embryos on their development

Published online by Cambridge University Press:  05 March 2014

Sung-Sik Kang ,
Sosuke Ofuji ,
Kei Imai ,
Weiping Huang ,
Keisuke Koyama ,
Yojiro Yanagawa ,
Yoshiyuki Takahashi  and
Masashi Nagano
Sung-Sik Kang
Affiliation:
Institute: Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060–0818, Japan.
Sosuke Ofuji
Affiliation:
Institute: Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060–0818, Japan.
Kei Imai
Affiliation:
National Livestock Breeding Center, Nishigo, Fukushima 961–8511, Japan.
Weiping Huang
Affiliation:
Institute: Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060–0818, Japan.
Keisuke Koyama
Affiliation:
Institute: Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060–0818, Japan.
Yojiro Yanagawa
Affiliation:
Institute: Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060–0818, Japan.
Yoshiyuki Takahashi
Affiliation:
Institute: Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060–0818, Japan. Genetics Hokkaido Association, Sapporo 060–0040, Japan.
Masashi Nagano*
Affiliation:
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita-ku Kita 18 Nishi 9, Sapporo 060–0818, Japan.
*
All correspondence to: M. Nagano. Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita-ku Kita 18 Nishi 9, Sapporo 060–0818, Japan. Tel:/Fax: +81 11 706 5231. e-mail: mnaga@vetmed.hokudai.ac.jp
Get access Rights & Permissions [Opens in a new window]

Summary

The aim of the present study was to clarify the efficacy of the well of the well (WOW) culture system for a small number of embryos and the effect of number of adjacent embryos in a WOW dish on blastocyst development. In conventional droplet culture, embryos in the small-number group (5–6 embryos/droplet) showed low blastocyst development compared with a control group (25–26 embryos/droplet). However, small and large numbers of embryos (5–6 and 25 embryos, respectively) in a WOW dish showed no significant differences in cleavage, blastocyst rates, and mean cell number in blastocysts compared with the control group (25–30 embryos/droplet). In addition, the number of adjacent embryos in a WOW dish did not affect the development to blastocysts and cell number in blastocysts. In conclusion, a WOW dish can provide high and stable blastocyst development in small group culture wherever embryos are placed in microwells of the WOW dish.

Keywords

BovineEmbryonic DevelopmentDropletIVCWOW
Type
Research Article
Information
Zygote , Volume 23 , Issue 3 , June 2015 , pp. 412 - 415
Copyright
Copyright © Cambridge University Press 2014 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Donnay, I., Van Langendonckt, A., Auquier, P., Grisart, B., Vansteenbrugge, A., Massip, A. & Dessy, F. (1997). Effects of co-culture and embryo number on the in vitro development of bovine embryos. Theriogenology 47, 1549–61.Google Scholar
Gopichandran, N. & Leese, H.J. (2006). The effect of paracrine/autocrine interactions on the in vitro culture of bovine preimplantation embryos. Reproduction 131, 269–77.Google Scholar
Hasler, J.F. (1998). The current status of oocytes recovery, in vitro embryo production, and embryo transfer in domestic animals, with an emphasis on the bovine. J. Anim. Sci. 76, 52–74.CrossRefGoogle Scholar
Ikeda, K., Takahashi, Y. & Katagiri, S. (2000). Effect of medium change on the development of in vitro matured and fertilized bovine oocytes cultured in medium containing amino acids. J. Vet. Med. Sci. 62, 121–3.Google Scholar
Krisher, R.L. & Wheeler, M.B. (2010). Towards the use of microfluidics for individual embryo culture. Reprod. Fertil. Dev. 22, 32–9.Google Scholar
Linder, G.M. & Wright, R.W. Jr. (1983). Bovine embryo morphology and evaluation. Theriogenology 20, 407–16.Google Scholar
Matoba, S., Fair, T. & Lonergan, P. (2010). Maturation, fertilisation and culture of bovine oocytes and embryos in an individually identifiable manner: a tool for studying oocyte developmental competence. Reprod. Fertil. Dev. 22, 839–51.Google Scholar
Merton, J.S., Ask, B., Onkundi, D.C., Mullaart, E., Colenbrander, B. & Nielen, M. (2009). Genetic parameters for oocyte number and embryo production within a bovine ovum pick-up-in vitro production embryo-production program. Theriogenology 72, 885–93.Google Scholar
Nagao, Y., Iijima, R. & Saeki, K. (2008). Interaction between embryos and culture conditions during in vitro development of bovine early embryos. Zygote 16, 127–33.Google Scholar
Senatore, E.M., Xu, J., Suárez Novoa, M.V., Gong, G., Lin, T., Bella, A., Moreno, J.F., Mannino, M.E., Tian, X., Presicce, G.A., Wu, S.-C. & Du, F. (2010). Improved in vitro development of OPU-derived bovine (Bos taurus) embryos by group culture with agarose-embedded helper embryos. Theriogenology 74, 1643–51.CrossRefGoogle ScholarPubMed
Stokes, P. J., Abeydeera, L. R. & Leese, H. J. (2005). Development of porcine in vivo and in vitro; evidence for embryo ‘cross talk’ in vitro . Dev. Biol. 284, 62–71.Google Scholar
Sugimura, S., Akai, T., Somfai, T., Hirayama, M., Aikawa, Y., Ohtake, M., Hattori, H., Kobayashi, S., Hashiyada, Y., Konishi, K. & Imai, K. (2010). Time lapse cinematography-compatible polystyrene-based microwell culture system: A novel tool for the development of individual bovine embryos. Biol. Reprod. 83, 970–8.CrossRefGoogle ScholarPubMed
Sugimura, S., Akai, M., Hashiyada, Y., Aikawa, Y., Ohtake, M., Matsuda, H., Kobayashi, S., Kobayashi, E., Konishi, K. & Imai, K. (2013). Effect of embryo density on in vitro development and gene expression in bovine in vitro-fertilized embryos cultured in a microwell system. J. Reprod. Dev. 59, 115–22.Google Scholar
Swain, J.E. & Smith, G.D. (2011). Advances in embryo culture platforms: novel approaches to improve preimplantation embryo development through modifications of the microenvironment. Hum. Reprod. Update 17, 541–57.CrossRefGoogle ScholarPubMed
Takahashi, Y. & First, N.L. (1992). In vitro development of bovine one-cell embryos: influence of glucose, lactate, pyruvate, animo acids and vitamins. Theriogenology 37, 963–78.CrossRefGoogle Scholar
Takahashi, Y. & Kanagawa, Y. (1998). Effects of glutamine, glycine and taurine on the development of in vitro fertilized bovine zygotes in a chemically defined medium. J. Vet. Med. Sci. 60, 433–7.CrossRefGoogle Scholar
Takahashi, Y., Hishinuma, M., Matsui, M., Tanaka, H. & Kanagawa, H. (1996). Development of in vitro matured/fertilized bovine embryos in a chemically defined medium: influence of oxygen concentration in the gas atmosphere. J. Vet. Med. Sci. 58, 897–902.Google Scholar
Vajta, G. (2010). Embryo culture: can we perform better than nature?. Reprod. Biomed. Online 20, 453–69.Google Scholar
Vajta, G., Peura, T.T., Holm, P., Paldi, A., Greve, T., Trounson, A.O. & Challensen, H. (2000). New method for culture of zona-included or zona-free embryos: the well of the well (WOW) system. Mol. Reprod. Dev. 55, 256–64.Google Scholar
Ward, F. A., Lonergan, P., Enright, B. P. & Boland, M. P. (2000). Factors affecting recovery and quality of oocytes for bovine embryo production in vitro using ovum pick-up technology. Theriogenology 54, 433–46.Google Scholar