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The efficacy of the well of the well (WOW) culture system on development of bovine embryos in a small group and the effect of number of adjacent embryos on their development

Published online by Cambridge University Press:  05 March 2014

Sung-Sik Kang ,
Sosuke Ofuji ,
Kei Imai ,
Weiping Huang ,
Keisuke Koyama ,
Yojiro Yanagawa ,
Yoshiyuki Takahashi  and
Masashi Nagano
Sung-Sik Kang
Affiliation:
Institute: Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060–0818, Japan.
Sosuke Ofuji
Affiliation:
Institute: Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060–0818, Japan.
Kei Imai
Affiliation:
National Livestock Breeding Center, Nishigo, Fukushima 961–8511, Japan.
Weiping Huang
Affiliation:
Institute: Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060–0818, Japan.
Keisuke Koyama
Affiliation:
Institute: Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060–0818, Japan.
Yojiro Yanagawa
Affiliation:
Institute: Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060–0818, Japan.
Yoshiyuki Takahashi
Affiliation:
Institute: Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060–0818, Japan. Genetics Hokkaido Association, Sapporo 060–0040, Japan.
Masashi Nagano*
Affiliation:
Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita-ku Kita 18 Nishi 9, Sapporo 060–0818, Japan.
*
All correspondence to: M. Nagano. Laboratory of Theriogenology, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita-ku Kita 18 Nishi 9, Sapporo 060–0818, Japan. Tel:/Fax: +81 11 706 5231. e-mail: mnaga@vetmed.hokudai.ac.jp
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Summary

The aim of the present study was to clarify the efficacy of the well of the well (WOW) culture system for a small number of embryos and the effect of number of adjacent embryos in a WOW dish on blastocyst development. In conventional droplet culture, embryos in the small-number group (5–6 embryos/droplet) showed low blastocyst development compared with a control group (25–26 embryos/droplet). However, small and large numbers of embryos (5–6 and 25 embryos, respectively) in a WOW dish showed no significant differences in cleavage, blastocyst rates, and mean cell number in blastocysts compared with the control group (25–30 embryos/droplet). In addition, the number of adjacent embryos in a WOW dish did not affect the development to blastocysts and cell number in blastocysts. In conclusion, a WOW dish can provide high and stable blastocyst development in small group culture wherever embryos are placed in microwells of the WOW dish.

Keywords

BovineEmbryonic DevelopmentDropletIVCWOW
Type
Research Article
Information
Zygote , Volume 23 , Issue 3 , June 2015 , pp. 412 - 415
Copyright
Copyright © Cambridge University Press 2014 

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