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Enhancement of histone acetylation by trichostatin A during in vitro fertilization of bovine oocytes affects cell number of the inner cell mass of the resulting blastocysts

Published online by Cambridge University Press:  01 August 2009

Shuntaro Ikeda ,
Atsuhiro Tatemizo ,
Daisaku Iwamoto ,
Shunji Taniguchi ,
Yoichiro Hoshino ,
Tomoko Amano ,
Kazuya Matsumoto ,
Yoshihiko Hosoi ,
Akira Iritani  and
Kazuhiro Saeki
Shuntaro Ikeda
Affiliation:
Department of Genetic Engineering, Kinki University, Wakayama, Japan.
Atsuhiro Tatemizo
Affiliation:
Department of Genetic Engineering, Kinki University, Wakayama, Japan.
Daisaku Iwamoto
Affiliation:
Department of Genetic Engineering, Kinki University, Wakayama, Japan.
Shunji Taniguchi
Affiliation:
Wakayama Prefecture Livestock Experimental Station, Wakayama, Japan.
Yoichiro Hoshino
Affiliation:
Gifu Prefectural Livestock Research Institute, Gifu, Japan.
Tomoko Amano
Affiliation:
Department of Genetic Engineering, Kinki University, Wakayama, Japan.
Kazuya Matsumoto
Affiliation:
Department of Genetic Engineering, Kinki University, Wakayama, Japan.
Yoshihiko Hosoi
Affiliation:
Department of Genetic Engineering, Kinki University, Wakayama, Japan.
Akira Iritani
Affiliation:
Department of Genetic Engineering, Kinki University, Wakayama, Japan.
Kazuhiro Saeki*
Affiliation:
Department of Genetic Engineering, Kinki University, Wakayama, 6496493, Japan. Department of Genetic Engineering, Kinki University, Wakayama, Japan.
*
All correspondence to: Kazuhiro Saeki. Department of Genetic Engineering, Kinki University, Wakayama, 6496493, Japan. Tel: +81 736 77 3888. Fax: +81 736 77 4754. e-mail: saeki@waka.kindai.ac.jp
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Summary

Histone acetylation is one of the major mechanisms of epigenetic reprogramming of gamete genomes after fertilization to establish a totipotent state for normal development. In the present study, the effects of trichostatin A (TSA), an inhibitor of histone deacetylase, during in vitro fertilization (IVF) of bovine oocytes on subsequent embryonic development were investigated. Cumulus-enclosed oocytes obtained from slaughterhouse bovine ovaries were matured in vitro and subjected to IVF in a defined medium supplemented with 0 (control), 5, 50, and 500 nM TSA for 18 h. After IVF, presumptive zygotes were cultured in modified synthetic oviductal fluid (mSOF) medium until 168 h postinsemination (hpi). Some oocytes were immunostained using antibody specific for histone H4-acetylated lysine 5 at 10 hpi. Cleavage, blastocyst development and cell number of inner cell mass (ICM) and trophectoderm (TE) of blastocysts were assessed. TSA treatment enhanced histone acetylation that was prominent in decondensed sperm nuclei. TSA did not affect the postfertilization cleavage, blastocyst rates, and TE cell number. However, it significantly enhanced ICM cell number (p < 0.05). These results indicate that TSA treatment during IVF of bovine oocytes does not affect blastocyst development but alters the cell number of ICM, suggesting that overriding epigenetic modification of the genome during fertilization has a carryover effect on cell proliferation and differentiation in preimplantation embryos. Thus, further environmental quality controls in assisted reproductive technologies are needed in terms of factors which affect chromatin remodelling.

Keywords

BlastocystsChromatin remodellingHistone acetylationIn vitro fertilizationTrichostatin A
Type
Research Article
Information
Zygote , Volume 17 , Issue 3 , August 2009 , pp. 209 - 215
Copyright
Copyright © Cambridge University Press 2009

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