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Estimation by radiation inactivation of the minimum functional size of acrosome-reaction-including substance (ARIS) in the starfish, Asterias amurensis

Published online by Cambridge University Press:  26 September 2008

Akira Ushiyama ,
Kazuyoshi Chiba ,
Akihiro Shima  and
Motonori Hoshi
Akira Ushiyama
Affiliation:
Tokyo Insitute of Technology, and University of Tokyo, Tokyo, Japan.
Kazuyoshi Chiba
Affiliation:
Tokyo Insitute of Technology, and University of Tokyo, Tokyo, Japan.
Akihiro Shima
Affiliation:
Tokyo Insitute of Technology, and University of Tokyo, Tokyo, Japan.
Motonori Hoshi*
Affiliation:
Tokyo Insitute of Technology, and University of Tokyo, Tokyo, Japan.
*
Motonori Hoshi, Department of Life Science, Tokyo Institute of Technology, Midori-ku, Yokohama 226, Japan. Telephone: 81-45-924-5720. Fax: 81-45-924-5777. e-mail: mhoshi@bio.titech.ac.jp.
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Summary

In the starfish Asterias amurensis, the jelly coat of the eggs contains a glycoprotein essential for the induction of the acrosome reaction in homologous spermatozoa that is termed the acrosome-reaction-inducing substance (ARIS).ARIS is a highly sulphated and fucose-rich glycoprotein of extremely high molecular mass(>104 kDa). ARIS was irradiated with high-energy eletrons in order to estimate the minimum size required for its biological activity. The minimum functional unit or target size of ARIS was estimated to be c. 14 kDa by target size analysis. ARIS was significantly disintegrated by the irradiation, yet the total sugar content was not apparently reduced. The binding of 125I-labelled ARIS to spermatozoa competed with that of irradiated ARIS, although the affinity of ARIS was much reduced after irradiation.

Keywords

Acrosome reactionEgg-coatSpermTarget analysis
Type
Article
Information
Zygote , Volume 3 , Issue 4 , November 1995 , pp. 351 - 355
Copyright
Copyright © Cambridge University Press 1995

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