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Evaluation of two sperm antigens, rSMP-B and YWK-II, as targets for immunocontraception

Published online by Cambridge University Press:  14 May 2001

Masaya Takikawa ,
Masaharu Kamada ,
Masahiko Maegawa ,
Shuji Yamano ,
Minoru Irahara ,
Toshihiro Aono ,
Siroh Futaki ,
Yasukazu Ohmoto  and
Samuel S. Koide
Masaya Takikawa
Affiliation:
Department of Obstetrics and Gynecology, School of Medicine, The University of Tokushima, Tokushima, Japan.
Masaharu Kamada
Affiliation:
Department of Obstetrics and Gynecology, School of Medicine, The University of Tokushima, Tokushima, Japan.
Masahiko Maegawa
Affiliation:
Department of Obstetrics and Gynecology, School of Medicine, The University of Tokushima, Tokushima, Japan.
Shuji Yamano
Affiliation:
Department of Obstetrics and Gynecology, School of Medicine, The University of Tokushima, Tokushima, Japan.
Minoru Irahara
Affiliation:
Department of Obstetrics and Gynecology, School of Medicine, The University of Tokushima, Tokushima, Japan.
Toshihiro Aono
Affiliation:
Department of Obstetrics and Gynecology, School of Medicine, The University of Tokushima, Tokushima, Japan.
Siroh Futaki
Affiliation:
Pharmaceutical Science, The University of Tokushima, Tokushima, Japan.
Yasukazu Ohmoto
Affiliation:
Cellular Technology Institute, Otsuka Pharmaceutical Co., Ltd, Tokushima, Japan.
Samuel S. Koide
Affiliation:
Center for Biomedical Research, The Population Council, New York, USA.
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Abstract

To determine whether sperm membrane components, rSMP-B and YWK-II, are suitable candidates as immunocontraceptives in humans, antifertility activities of the antibodies to the peptide fragments, rSMP-229 and rSMP-230 of rSMP-B and YAL-198 of YWK-II, were examined. In a previous report, anti-rSMP-230 antibody was shown to immobilise human sperm and to block human fertilisation, and the antigen (rSMP-230) to interact with antisperm antibodies found in sera of infertile women. Antibody to the second synthetic peptide, rSMP-229, corresponding to a different segment of rSMP-B, mimicked the biological activities of the anti-rSMP-230 antibody. Anti-YAL-198 antibody significantly, although weakly, inhibited human fertilisation. In the murine model, the anti-rSMP-B antibodies blocked in vitro fertilisation of mouse eggs but had no influence on embryo growth. Anti-YAL-198 antibody, however, arrested the growth of zygotes. In conclusion, rSMP-B, a human sperm protein, is a promising candidate in the development of an immunocontraceptive for human application. A second sperm protein, YWK-II, is effective as an antifertility immunogen in experimental animals.

Keywords

Antisperm antibodyContraceptionEmbryo growthFertilisationHuman sperm protein
Type
Research Article
Information
Zygote , Volume 9 , Issue 2 , May 2001 , pp. 145 - 151
Copyright
2001 Cambridge University Press

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