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Fibroblast growth factor-2 promotes in vitro activation of cat primordial follicles

Published online by Cambridge University Press:  13 April 2022

M.C. Müller ,
A.P.O. Monte ,
T.L.B.G. Lins ,
T.J.S. Macedo ,
V.R.P. Barros ,
V.C. Ferreira ,
D. Baraúna Jr. ,
C.R.O. Santos ,
A.R. Silva  and
M.H.T. Matos Open the ORCID record for M.H.T. Matos [Opens in a new window]
M.C. Müller
Affiliation:
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil
A.P.O. Monte
Affiliation:
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil
T.L.B.G. Lins
Affiliation:
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil
T.J.S. Macedo
Affiliation:
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil
V.R.P. Barros
Affiliation:
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil
V.C. Ferreira
Affiliation:
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil
D. Baraúna Jr.
Affiliation:
University Veterinary Clinic, Department of Veterinary Medicine, Federal University of São Francisco Valley, Petrolina, PE, Brazil
C.R.O. Santos
Affiliation:
University Veterinary Clinic, Department of Veterinary Medicine, Federal University of São Francisco Valley, Petrolina, PE, Brazil
A.R. Silva
Affiliation:
Laboratory of Animal Germplasm Conservation (LCGA), Universidade Federal Rural do Semi-Árido, Mossoró, RN, Brazil
M.H.T. Matos*
Affiliation:
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Federal University of São Francisco Valley, Petrolina, PE, Brazil
*
Author for correspondence: M.H.T. Matos. Universidade Federal do Vale do São Francisco (UNIVASF). Colegiado de Medicina Veterinária – Laboratório de Biologia Celular, Citologia e Histologia. Rodovia BR 407, Km 12, Lote 543 – Projeto de Irrigação Nilo Coelho – S/N, C1. CEP: 56300–990 – Petrolina – PE – Brasil. E-mail: helena.matos@univasf.edu.br
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Summary

This study evaluated the effect of fibroblast growth factor-2 (FGF-2) on the morphology, primordial follicle activation and growth after in vitro culture of domestic cat ovarian tissue. Ovaries (n = 12) from prepubertal domestic cats were collected and fragmented. One fragment was fixed for histological analysis (fresh control). The remaining fragments were incubated in control medium alone or with 10, 50 or 100 ng/ml FGF-2 for 7 days. After in vitro culture, the following endpoints were analyzed: morphology, activation by counting primordial and developing follicles, and growth (follicle and oocyte diameters). Treatment with 100 ng/ml FGF-2 maintained (P > 0.05) the percentage of normal follicles similar to fresh control. Follicle survival was greater (P < 0.05) after culture in 100 ng/ml FGF-2 than in 50 ng/ml FGF-2. The percentage of primordial follicles decreased (P < 0.05) and the percentage of developing follicles increased (P < 0.05) in all treatments compared with fresh tissue. The proportion of developing follicles increased (P < 0.05) in tissues incubated with 100 ng/ml FGF-2 compared with control medium and other FGF-2 concentrations. Furthermore, culture in 10 or 100 ng/ml FGF-2 resulted in increased (P < 0.05) follicle and oocyte diameters compared with fresh tissues and MEM+. In conclusion, FGF-2 at 100 ng/ml maintains follicle survival and promotes the in vitro activation and growth of cat primordial follicles.

Keywords

FelineFGF-2FolliculogenesisParacrine factorViability
Type
Short Communication
Information
Zygote , Volume 30 , Issue 5 , October 2022 , pp. 730 - 734
Copyright
© The Author(s), 2022. Published by Cambridge University Press

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