Hostname: page-component-78c5997874-ndw9j Total loading time: 0 Render date: 2024-11-10T20:46:14.272Z Has data issue: false hasContentIssue false
  • English
  • Français

Lower apoptosis rate in ovine preantral follicles from ovaries stored in supplemented preservation media

Published online by Cambridge University Press:  28 January 2015

R.J.S. Gonçalves ,
A.Y.P. Cavalcante ,
B.B. Gouveia ,
T.L.B. Lins ,
R.S. Barberino ,
V.G. Menezes ,
V.R.P. Barros ,
T.J.S. Macedo ,
J.R. Figueiredo  and
M.H.T. Matos
R.J.S. Gonçalves
Affiliation:
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Laboratory of Cell Biology, Cytology and Histology, Federal University of San Francisco Valley (UNIVASF), Petrolina, Brazil.
A.Y.P. Cavalcante
Affiliation:
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Laboratory of Cell Biology, Cytology and Histology, Federal University of San Francisco Valley (UNIVASF), Petrolina, Brazil.
B.B. Gouveia
Affiliation:
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Laboratory of Cell Biology, Cytology and Histology, Federal University of San Francisco Valley (UNIVASF), Petrolina, Brazil.
T.L.B. Lins
Affiliation:
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Laboratory of Cell Biology, Cytology and Histology, Federal University of San Francisco Valley (UNIVASF), Petrolina, Brazil.
R.S. Barberino
Affiliation:
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Laboratory of Cell Biology, Cytology and Histology, Federal University of San Francisco Valley (UNIVASF), Petrolina, Brazil.
V.G. Menezes
Affiliation:
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Laboratory of Cell Biology, Cytology and Histology, Federal University of San Francisco Valley (UNIVASF), Petrolina, Brazil.
V.R.P. Barros
Affiliation:
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Laboratory of Cell Biology, Cytology and Histology, Federal University of San Francisco Valley (UNIVASF), Petrolina, Brazil.
T.J.S. Macedo
Affiliation:
Nucleus of Biotechnology Applied to Ovarian Follicle Development, Laboratory of Cell Biology, Cytology and Histology, Federal University of San Francisco Valley (UNIVASF), Petrolina, Brazil.
J.R. Figueiredo
Affiliation:
Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocyte and Preantral Follicles (LAMOFOPA), State University of Ceara, 60740-000, Fortaleza-CE, Brazil.
M.H.T. Matos*
Affiliation:
Universidade Federal do Vale do São Francisco (UNIVASF), Colegiado de Medicina Veterinária – Laboratório de Biologia Celular, Citologia e Histologia, Rodovia BR 407, Km 12, Lote 543 – Projeto de Irrigação Nilo Coelho – S/N, C1 CEP: 56300-990 – Petrolina – PE–Brasil.
*
All correspondence to: M.H.T. Matos. Universidade Federal do Vale do São Francisco (UNIVASF), Colegiado de Medicina Veterinária – Laboratório de Biologia Celular, Citologia e Histologia, Rodovia BR 407, Km 12, Lote 543 – Projeto de Irrigação Nilo Coelho – S/N, C1 CEP: 56300-990 – Petrolina – PE–Brasil. Tel.: +55 87 2101 4839. e-mail: helena.matos@univasf.edu.br
Get access Rights & Permissions [Opens in a new window]

Summary

The aim of this study was to investigate the effect of ovarian tissue transportation conditions (medium and period of time) on the morphology, apoptosis and development of ovine preantral follicles cultured in vitro. Each ovarian pair was cut into nine slices, with one fragment being fixed immediately (fresh control). The remaining fragments were placed individually in cryotubes containing conservation medium (minimal essential medium (MEM) without supplementation or MEM+ – with supplementation) and stored at 35ºC for 6 or 12 h without (non-cultured) or with subsequent culture for 5 days. Then, the fragments were processed for histological and terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labelling (TUNEL) examination. Preservation of ovarian slices in MEM or MEM+ (non-cultured) resulted in similar percentages of normal follicles when compared with the fresh control. Nevertheless, compared with the fresh control, a decrease in the percentage of normal follicles was observed in tissues cultured for 5 days. Only for tissues preserved in supplemented medium (MEM+) for 6 h, the percentage of TUNEL positive cells was similar between non-cultured tissues and tissues cultured for 5 days. Follicular activation and growth (follicular and oocyte diameter) were higher in cultured tissues than in fresh control or non-cultured tissues, except those from fragments preserved for 6 h in MEM and then cultured for 5 days in which no growth was observed. In conclusion, ovine ovarian tissue was successfully preserved in supplemented medium (MEM+) at a temperature close to physiological values (35°C) for up to 6 h without affecting apoptosis in the ovarian follicles and their ability to develop in vitro.

Keywords

GrowthOvaryPreservationSheepSurvival
Type
Research Article
Information
Zygote , Volume 23 , Issue 6 , December 2015 , pp. 943 - 950
Copyright
Copyright © Cambridge University Press 2015 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abd-Allah, S.M. (2010). Effects of storage conditions of dromedary camel ovaries on the morphology, viability and development of antral follicular oocytes. Anim. Reprod. 7, 65–9.Google Scholar
Abedelahi, A., Salehnia, M., Allameh, A.A. & Davoodi, D. (2010). Sodium selenite improves the in vitro follicular development by reducing the reactive oxygen species level and increasing the total antioxidant capacity and glutathione peroxide activity. Hum. Reprod. 25, 977–85.Google Scholar
Andrade, E.R., Rodrigues, A.P.R., Amorim, C.A., Carvalho, F.C.A., Dode, M.A.N. & Figueiredo, J.R. (2002). Short term maintenance of sheep preantral follicles in situ in 0.9% saline and Braun-Collins solution. Small Rum. Res. 41, 141–9.Google Scholar
Byrd, S.R., Flores-Foxworth, G. & Westhusin, M.E. (1997). In vitro maturation of ovine oocyte in a portable incubator. Theriogenology 47, 857–64.Google Scholar
Celestino, J.J.H., Santos, R.R., Martins, F.S., Matos, M.H.T., Figueiredo, J.R., Costa, S.H.F., Silva, J.R.V. & Rodrigues, A.P.R. (2007). Conservação de folículos pré-antrais bovinos em solução salina 0,9% ou TCM 199. Arq. Bras. Med. Vet. Zoot. 59, 591–9.Google Scholar
Chaves, R.N., Martins, F.S., Saraiva, M.V.A., Celestino, J.J.H., Lopes, C.A.P., Correia, J.C., Lima Verde, I.B., Matos, M.H.T., Báo, S.N., Name, K.P.O., Campello, C.C. & Figueiredo, J.R. (2008). Chilling ovarian fragments during transportation improves viability and growth of goat preantral follicles cultured in vitro . Reprod. Fertil. Dev. 20, 640–7.Google Scholar
Evecen, M., Cirit, U., Demir, K., Ozdas, O.B., Tas, M., Birler, S. & Pabuccuiglu, S. (2010). Effects of estrous cycle stage and transport temperature of ovaries on in vitro maturation of canine oocytes. Anim. Reprod. Sci. 117, 160–5.Google Scholar
García-Álvarez, O., Maroto-Morales, A., Berlinguer, F., Fernández-Santos, M.R., Esteso, M.C., Mermillod, P., Ortiz, J.Á., Ramon, M., Pérez-Guzmán, M.D., Garde, J.J. & Soler, A.J. (2011). Effect of storage temperature during transport of ovaries on in vitro embryo production in Iberian red deer (Cervus elaphus hispanicus). Theriogenology 75, 65–72.Google Scholar
Love, L.B., Choi, Y.H., Love, C.C., Varner, D.D. & Hinrichs, K. (2003). Effect of ovary storage and oocyte transport method on maturation rate of horse oocytes. Theriogenology 59, 765–74.Google Scholar
Martins, F.S., Van den Hurk, R., Santos, R.R., Silva, J.R.V., Matos, M.H.T., Celestino, J.J.H., Rodrigues, A.P.R., Pessoa, C., Ferreira, F.V.A. & Figueiredo, J.R. (2005). Development of goat primordial follicles after in vitro culture of ovarian tissue in Minimal Essential Medium supplemented with coconut water. Anim. Reprod. 2, 106–13.Google Scholar
Matos, M.H.T., Andrade, E.R., Lucci, C.M., Báo, S.N., Silva, J.R.V., Santos, R.R., Ferreira, M.A.L., Costa, S.H.F., Celestino, J.J.H. & Figueiredo, J.R. (2004). Morphological and ultrastructural analysis of sheep primordial follicles preserved in 0.9% saline solution and TCM 199. Theriogenology 62, 65–80.Google Scholar
Peng, X., Yang, M., Wang, L., Tong, C. & Guo, Z. (2010). In vitro culture of sheep lamb ovarian cortical tissue in a sequential culture medium. J. Assist. Reprod. Genet. 27, 247–57.CrossRefGoogle Scholar
Quan, F., Zhiping, Z., Zhixing, A., Song, H. Min, W., Xiao'e, Zhao & Yong, Z. (2010). Effect of transporting donor or recipient does and their embryos on the outcome of fresh embryo transfer in Boer goats. Small Rumin. Res. 88, 1–5.Google Scholar
Rodrigues, G.Q., Silva, C.M.G., Faustino, L.R., Bruno, J.B., Magalhães, D.M., Campello, C.C. & Figueiredo, J.R. (2010). Bovine serum albumin improves in vitro development of caprine preantral follicles. Anim. Reprod. 7, 382–8.Google Scholar
Saha, S., Shimizu, M., Geshi, M. & Izaike, Y. (2000). In vitro culture of bovine preantral follicles. Anim. Reprod. Sci. 63, 27–39.Google Scholar
Santos, J.M.S., Menezes, V.G., Barberino, R.S., Macedo, T.J.S., Lins, T.L.B., Gouveia, B.B., Barros, V.R.P., Santos, L.P., Gonçalves, R.J.S. & Matos, M.H.T. (2014). Immunohistochemical localization of fibroblast growth factor-2 in the sheep ovary and its effects on pre-antral follicle apoptosis and development in vitro . Reprod. Dom. Anim. 49, 522–8.Google Scholar
Shirazi, A., Ostad-Hosseini, S., Ahmadi, E., Heidari, B. & Shams-Esfandabadi, N. (2009). In vitro developmental competence of ICSI derived activated ovine embryos. Theriogenology 71, 342–8.Google Scholar
Silva, J.R.V., Lucci, C.M., Carvalho, F.C.A., Báo, S.N., Costa, S.H.F., Santos, R.R. & Figueiredo, J.R. (2000). Effect of coconut water and Braun-Collins solutions at different temperatures and incubation times on the morphology of goat preantral follicles preserved in vitro . Theriogenology 54, 809–22.CrossRefGoogle ScholarPubMed
Silva, J.R.V., van den Hurk, R., Costa, S.H.F., Andrade, E.R., Nunes, A.P.A., Ferreira, F.V.A., Lôbo, R.N.B. & Figueiredo, J.R. (2004). Survival and growth of goat primordial follicles after in vitro culture of ovarian cortical slices in media containing coconut water. Anim. Reprod. Sci. 81, 273–86.Google Scholar
Songsasen, N., Spindler, R.E. & Wildt, D.E. (2007). Requirement for, and patterns of, pyruvate and glutamine metabolism in the domestic dog oocyte in vitro . Mol. Reprod. Dev. 74, 870–7.Google Scholar
Sreejalekshmi, P., Raghavendra, B.S., Subramani, T.S., Murthy, V.C., Jamuna, K.V., Prasad, R.V., Ravindra, J.P. & Selvaraju, S. (2011). Detection of follicular apoptosis in water buffalo (Bubalus bubalis) ovary by histology and nick end labelling technique. Reprod. Domest. Anim. 46, 59–65.Google Scholar
Tellado, M.N, Alvarez, G.M., Dalvit, G.C. & Cetica, P.D. (2014). The conditions of ovary storage affect the quality of porcine oocytes. Adv. Reprod. Sci. 2, 57–67.Google Scholar
Wan, P.C., Hao, Z.D., Zhou, P., Wu, Y., Yang, L., Cui, M.S., Lui, S.R. & Zeng, S.M. (2009). Effects of SOF and CR1 media on developmental competence and cell apoptosis of ovine in vitro fertilization embryos. Anim. Reprod. Sci. 114, 279–88.CrossRefGoogle ScholarPubMed
Wongsrikeao, P., Otoi, T., Karja, N.W.K., Agung, B., Nii, M. & Nagai, T. (2005). Effects of ovary time and temperature on DNA fragmentation and development of porcine oocytes. J. Reprod. Dev. 51, 87–97.Google Scholar