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Male pronuclear formation and sperm mitochondria in porcine oocytes following intracytoplasmic injection of pig or mouse sperm

Published online by Cambridge University Press:  19 June 2002

Youn-Jeong Lee ,
Bong-Ki Kim ,
Xiang Shun Cui  and
Nam-Hyung Kim
Youn-Jeong Lee
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheong Ju, Chungbuk, Korea
Bong-Ki Kim
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheong Ju, Chungbuk, Korea
Xiang Shun Cui
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheong Ju, Chungbuk, Korea
Nam-Hyung Kim
Affiliation:
Department of Animal Sciences, Chungbuk National University, Cheong Ju, Chungbuk, Korea Research Center for Bioresource and Health, Chungbuk National University, Cheong Ju, Chungbuk, Korea
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Abstract

In the present study we determined the chromatin organization and fate of introduced mitochondria in porcine embryos following intracytoplasmic injection of pig or mouse sperm cells. At 3, 6, 9 and 12 h following injection of pig or mouse spermatozoa or isolated sperm heads, the oocytes were fixed and stained with propidium iodide. Between 3 and 6 h following injection, both porcine and murine sperm chromatin developed into pronuclei. The male and female pronuclei were apposed within 12 h in porcine oocytes following sperm injection from either source. We also introduced foreign mitochondria from either mouse or pig sperm midpiece into porcine oocytes following sperm injection. While porcine sperm mitochondria rapidly disappeared from the actively developing porcine oocytes, mouse sperm mitochondria remained in the embryos until the 8-cell stage. These results suggest that pronuclear formation and movement occur between 6 and 12 h following sperm incorporation into the cytoplasm, and that foreign mitochondria are selectively removed in a species-specific manner.

Keywords

FertilisationMitochondriaMousePigPronucleus
Type
Research Article
Information
Zygote , Volume 10 , Issue 2 , May 2002 , pp. 117 - 122
Copyright
2002 Cambridge University Press

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