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Mitotic index and morphological characteristics of ovarian small follicles from goats submitted to nutritionally unbalanced regimens

Published online by Cambridge University Press:  24 August 2017

Davide Rondina ,
Vicente J.F. Freitas ,
Jamily B. Bruno ,
Juliana J.H. Celestino  and
Regiane R. Santos
Davide Rondina*
Affiliation:
Faculdade de Veterinária, Universidade Estadual do Ceará, Av. Silas Munguba, 1700. Campus do Itaperi, 60714–903, Fortaleza, Ceará, Brazil.
Vicente J.F. Freitas
Affiliation:
Faculdade de Veterinária, Universidade Estadual do Ceará, Fortaleza, Ceará, Brazil.
Jamily B. Bruno
Affiliation:
Faculdade de Veterinária, Universidade Estadual do Ceará, Fortaleza, Ceará, Brazil.
Juliana J.H. Celestino
Affiliation:
Institute of Health Sciences, Universidade da Integração Internacional da Lusofonia Afro-Brasileira. Acarape-CE, Brazil.
Regiane R. Santos
Affiliation:
Schothorst Feed Research, Lelystad, The Netherlands.
*
All correspondence to: Davide Rondina. Faculdade de Veterinária, Universidade Estadual do Ceará, Av. Silas Munguba, 1700. Campus do Itaperi, 60714–903, Fortaleza, Ceará, Brazil. Tel: +55 85 31019858. Fax: +55 85 31019840. E-mail: davide.rondina@uece.br
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Summary

The objective of this study was to assess the influence of nutritional regimens such as adequate feeding, restricted feeding, and underfeeding–refeeding on the follicle growth and development from caprine ovaries. Goats were divided into three different groups (n = 5 per group). For 24 weeks, goats received elephant grass plus concentrate to provide 1.5 (n = 5) and 0.72 (n = 10) times the energy requirements for maintenance of live weight. Underfed goats were subsequently refed for 6 weeks with the diet of the nourished group (1.5 times the energetic requirements of maintenance). Follicular morphology and morphometry, as well as granulosa cells mitotic index were assessed. Ovarian follicles were classified as small or large preantral follicles, or as small or large antral follicles. Ovarian volume was smaller in animals from both underfed and refed groups than in those animals from fed group. Although no difference in the total number of normal follicles was observed among the nutritional groups, underfed animals presented higher percentages of atretic preantral and small antral follicles when compared with fed animals. Large antral follicles from underfed and refed goats presented a lower mitotic index when compared with fed ones. In conclusion, ovaries from goats challenged with prolonged undernutrition will be functionally compromised, which is characterized by atresia of preantral and small antral follicles and decreased mitotic index of large antral follicles. Refeeding those animals will not recover ovarian function to a same level experienced by goats fed a diet with adequate energy requirements.

Keywords

MitosisMorphologyOvarySmall ruminantsUndernourishment
Type
Research Article
Information
Zygote , Volume 25 , Issue 5 , October 2017 , pp. 567 - 574
Copyright
Copyright © Cambridge University Press 2017 

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