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Modification of membrane cholesterol and its impact on frozen–thawed chicken sperm characteristics

Published online by Cambridge University Press:  03 May 2016

Agnieszka Partyka ,
Dorota Bonarska-Kujawa ,
Marta Sporniak ,
Maciej Strojecki  and
Wojciech Niżański
Agnieszka Partyka*
Affiliation:
Wroclaw University of Environmental and Life Sciences, Faculty of Veterinary Medicine, Department of Reproduction and Clinic of Farm Animals, pl. Grunwaldzki 49, 50–366 Wrocław, Poland.
Dorota Bonarska-Kujawa
Affiliation:
Wroclaw University of Environmental and Life Sciences, Faculty of Life Sciences and Technology, Department of Physics and Biophysics, ul. Norwida 25, 50–375 Wroclaw, Poland.
Marta Sporniak
Affiliation:
Wroclaw University of Environmental and Life Sciences, Faculty of Veterinary Medicine, Department of Reproduction and Clinic of Farm Animals, pl. Grunwaldzki 49, 50–366 Wroclaw, Poland.
Maciej Strojecki
Affiliation:
Wroclaw University of Environmental and Life Sciences, Faculty of Veterinary Medicine, Department of Reproduction and Clinic of Farm Animals, pl. Grunwaldzki 49, 50–366 Wroclaw, Poland.
Wojciech Niżański
Affiliation:
Wroclaw University of Environmental and Life Sciences, Faculty of Veterinary Medicine, Department of Reproduction and Clinic of Farm Animals, pl. Grunwaldzki 49, 50–366 Wroclaw, Poland.
*
All correspondence to: Agnieszka Partyka. Wroclaw University of Environmental and Life Sciences, Faculty of Veterinary Medicine, Department of Reproduction and Clinic of Farm Animals, pl. Grunwaldzki 49, 50–366 Wrocław, Poland. Tel: +48 71 32 05 300. Fax: +48 71 32 01 006. E-mail address: partykaagnieszka@gmail.com
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Summary

This study was conducted to determine the changes in chicken sperm plasma membranes fluidity and polarity as lipid packing arrangement induced by cholesterol-loaded cyclodextrin (CLC) and 2-hydroxypropyl-β-cyclodextrin (HBCD) and how sperm cryopreservation outcomes are improved by these changes. Treatment with 2 mg HBCD supported the highest (P < 0.01) percentage of viable spermatozoa compared with the control and CLCs groups after cryopreservation. The percentage of post-thaw progressive and rapid sperm motility was highest in 2 mg HBCD (P < 0.01). After thawing, sperm treated with 1 or 2 mg CLC showed the highest anisotropy at 5, 21, 25 and 40°C (P < 0.01). At 25°C, the lowest anisotropy was observed in the thawed semen from the control group. The highest value (P < 0.01) of generalized polarization (GP) (0.5) at 5°C was observed in the 1 mg CLC treated sample. After 2 h of incubation, the highest percentage of viable spermatozoa was observed in the HBCD group in relation to the other treatments (P < 0.01). Exposure to 1 mg or 2 mg of CLC significantly decreased the percentage of live spermatozoa after thawing (P < 0.01). In conclusion, HBCD appears to play a role in the modification of sperm membranes, increasing their fluidity and preventing them against membrane phase transition to gel, thus minimizing freezing-thaw sperm damage. HBCD treatment enhances chicken sperm viability and motility after cryopreservation and subsequent storage. This novel procedure may be useful for improving the technology for cryopreservation of fowl spermatozoa.

Keywords

Chicken sperm cholesterolCryopreservationCyclodextrinSemen analysisSperm membrane fluidity
Type
Research Article
Information
Zygote , Volume 24 , Issue 5 , October 2016 , pp. 714 - 723
Copyright
Copyright © Cambridge University Press 2016 

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