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Nitric-oxide-dependent activation of pig oocytes: the role of the cGMP-signalling pathway

Published online by Cambridge University Press:  24 March 2006

J. Petr ,
R. Rajmon ,
E. Chmelíková ,
M. Tománek ,
V. Lánská ,
M. Přibáňová  and
F. Jílek
J. Petr
Affiliation:
Research Institute of Animal Production, Přátelství 815, Prague 10, Czech Republic.
R. Rajmon
Affiliation:
Czech University of Agriculture in Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Veterinary Sciences, Kamýcká 129, Prague 6, Czech Republic.
E. Chmelíková
Affiliation:
Czech University of Agriculture in Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Veterinary Sciences, Kamýcká 129, Prague 6, Czech Republic.
M. Tománek
Affiliation:
Research Institute of Animal Production, Přátelství 815, Prague 10, Czech Republic.
V. Lánská
Affiliation:
Czech University of Agriculture in Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Veterinary Sciences, Kamýcká 129, Prague 6, Czech Republic.
M. Přibáňová
Affiliation:
Research Institute of Animal Production, Přátelství 815, Prague 10, Czech Republic.
F. Jílek
Affiliation:
Czech University of Agriculture in Prague, Faculty of Agrobiology, Food and Natural Resources, Department of Veterinary Sciences, Kamýcká 129, Prague 6, Czech Republic.
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Abstract

Pig oocytes matured in vitro were parthenogenetically activated (78%) after treatment with 2 mM nitric oxide-donor (±)-S-nitroso-N-acetylpenicillamine (SNAP) for 24 h. Inhibition of soluble guanylyl cyclase with the specific inhibitors 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) or 6-anilino-5,8-quinolinequinone (LY83583) suppressed the SNAP-induced activation in a dose-dependent manner (23% of activated oocytes after treatment with 400 μM ODQ; 12% of activated oocytes after treatment with 40 μM LY83583). 8-Bromo-cyclic guanosine monophosphate (8-Br-cGMP), a phosphodiesterase-resistant analogue of cGMP, enhances the effect of suboptimal doses (0.1 or 0.5 mM) of the NO donor SNAP. DT3, a specific inhibitor of cGMP-dependent protein kinase (PKG, PKG), is also able to inhibit the activation of pig oocytes after NO donor treatment. Involvement of the cGMP-dependent signalling pathway is specific for NO-induced oocyte activation, because both the guanylyl cyclase inhibitor ODQ and the PKG inhibitor DT3 are unable to inhibit activation in oocytes treated with the calcium ionophore A23187. These data indicate that the activation of pig oocytes with an NO donor is cGMP-dependent and that PKG plays an important role in this mode of oocyte activation.

Keywords

ActivationcGMPNitric oxidePig oocyte
Type
Research Article
Information
Zygote , Volume 14 , Issue 1 , February 2006 , pp. 9 - 16
Copyright
Cambridge University Press 2006

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