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OPS vitrification of mouse immature oocytes before or after meiosis: the effect on cumulus cells maintenance and subsequent development

Published online by Cambridge University Press:  01 February 2009

Lun Suo ,
Guang-Bin Zhou ,
Qing-Gang Meng ,
Chang-Liang Yan ,
Zhi-Qiang Fan ,
Xue-Ming Zhao ,
Xiang-Wei Fu ,
Yan-Ping Wang ,
Qing-Jing Zhang  and
Shi-En Zhu
Lun Suo
Affiliation:
Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural University, Beijing 100093, P.R. China.
Guang-Bin Zhou
Affiliation:
Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural University, Beijing 100093, P.R. China.
Qing-Gang Meng
Affiliation:
Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural University, Beijing 100093, P.R. China.
Chang-Liang Yan
Affiliation:
Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural University, Beijing 100093, P.R. China.
Zhi-Qiang Fan
Affiliation:
Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural University, Beijing 100093, P.R. China.
Xue-Ming Zhao
Affiliation:
Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural University, Beijing 100093, P.R. China.
Xiang-Wei Fu
Affiliation:
Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural University, Beijing 100093, P.R. China.
Yan-Ping Wang
Affiliation:
Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural University, Beijing 100093, P.R. China.
Qing-Jing Zhang
Affiliation:
Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural University, Beijing 100093, P.R. China.
Shi-En Zhu*
Affiliation:
Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural University, No. 2 West Yuanmingyuan Road, Haidian District, Beijing 100093, P.R. China. Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural University, Beijing 100093, P.R. China. State Key Laboratories for Agrobiotechnology, China Agricultural University, Beijing 100093, PR China.
*
All correspondence to: Shi-En Zhu. Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural University, No. 2 West Yuanmingyuan Road, Haidian District, Beijing 100093, P.R. China. Tel:/Fax: +86 10 6273 1767. e-mail: zhushien@cau.edu.cn
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Summary

Cryopreservation can cause cumulus cell damage around the immature oocytes, which may result in poor subsequent development. To evaluate the effect of the meiosis stage on the cumulus cell cryoinjury and determine the suitable stage for cryopreservation in immature oocytes, mouse oocytes at germinal vesicle (GV) and germinal vesicle breakdown (GVBD) stages were vitrified using open pulled straw (OPS) method. Cumulus cells damage was scored immediately after thawing by double-fluorescent staining. The survival rate of the oocytes was evaluated and the subsequent development of oocytes was assessed through in vitro culture (IVC) and in vitro fertilization (IVF) separately. After vitrification, a higher proportion of cumulus cells of GV oocytes were damaged than those of GVBD and untreated control groups. The survival rate of vitrified GVBD oocytes (94.1%) was significantly higher (p < 0.05) than that of GV oocytes (85.4%). Oocytes vitrified at GVBD stage (55.7%) showed similar cleavage rate compared to those at GV stage (49.2%), but significantly higher (p < 0.05) blastocyst rate (40.9% vs. 27.4%). These results demonstrate that oocytes at GVBD stage remain better cumulus membrane integrity and developmental ability during vitrification than those at GV stage, indicating they are more suitable for immature oocytes cryopreservation in mice.

Keywords

Cumulus cellsGerminal vesicleMouseOocyteVitrification
Type
Research Article
Information
Zygote , Volume 17 , Issue 1 , February 2009 , pp. 71 - 77
Copyright
Copyright © Cambridge University Press 2008

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References

Agca, Y., Liu, J., Peter, A.T., Critser, E.S. & Critser, J.K. (1998). Effect of developmental stage on bovine oocyte plasma membrane water and cryoprotectant permeability characteristics. Mol. Reprod. Dev. 49, 408–15.3.0.CO;2-R>CrossRefGoogle ScholarPubMed
Akiyama, T., Nagata, M. & Aoki, F. (2006). Inadequate histone deacetylation during oocyte meiosis causes aneuploidy and embryo death in mice. PNAS 103, 7339–44.CrossRefGoogle ScholarPubMed
Alworth, A.E. & Albertini, D.F. (1993). Meiotic maturation in cultured bovine oocytes is accompanied by remodeling of the cumulus cell cytoskeleton. Dev. Biol. 158, 101–12.CrossRefGoogle Scholar
Aman, R.R. & Parks, J.E. (1994). Effects of cooling and rewarming on the meiotic spindle and chromosomes of in vitro matured bovine oocytes. Biol. Reprod. 50, 103–10.CrossRefGoogle ScholarPubMed
Anderson, E. & Albertini, D.F. (1976). Gap junctions between the oocytes and companion follicle cells in the mammalian ovary. J. Cell Biol. 71, 680–6.CrossRefGoogle ScholarPubMed
Aono, N., Abe, Y., Hara, K., Sasada, H., Sato, E. & Yoshida, H. (2005). Production of live offspring from mouse germinal vesicle–stage oocytes vitrified by a modified stepwise method, SWEID, Fertil. Steril. 84 (Suppl. 2), 1078–82.CrossRefGoogle Scholar
Barnes, F.L., Daniani, P., Looney, C.R. & Duby, R.T. (1997). The meiotic stage affects subsequent development of cooled bovine oocytes. Theriogenology 47, 183.CrossRefGoogle Scholar
Byskov, A.G., Andersen, C.Y., Nordholm, L., Thogersen, H., Xia, G., Wassmann, O., Andersen, J.V., Guddal, E. & Roed, T. (1995). Chemical structure of sterols that activate oocyte meiosis. Nature 374, 559–62.CrossRefGoogle ScholarPubMed
Carmen, D., Paloma, D., Enrique, G., Carlos, O.H., Carolina, T., Aida, R., Lina, F., Santiago de la, V., Alba, F., Nieves, F. & Maite, C. (2005). Bovine oocyte vitrification before or after meiotic arrest: effects on ultrastructure and developmental ability. Theriogenology 64, 317–33.Google Scholar
Chen, S.U., Lien, Y.R., Chen, H.F., Chao, K.H., Ho, H.N. & Yang, Y.S. (2001). Open pulled straws for vitrification of mature mouse oocytes preserve patterns of meiotic spindles and chromosomes better than conventional straws. Hum. Reprod. 16, 1777–9.CrossRefGoogle Scholar
Chian, R.C., Niwa, K. & Sirard, M.A. (1994). Effects of cumulus cells on male pronuclear formation and subsequent early development of bovine oocytes in vitro. Theriogenology 41, 1499–508.CrossRefGoogle ScholarPubMed
Eppig, J.J. (1994). Oocyte–somatic cell communication in the ovarian follicles of mammals. Semin. Dev. Biol. 5, 51–9.CrossRefGoogle Scholar
Eroglu, A., Toner, M., Leykin, L. & Toth, T.L. (1998). Cytoskeleton and polyploidy after maturation and fertilization of cryopreserved germinal vesicle-stage mouse oocytes. J. Assist. Reprod. Genet. 15 (7), 447–54.CrossRefGoogle ScholarPubMed
Fagbohun, C.F. & Downs, S.M. (1991). Metabolic coupling and ligand stimulated meiotic maturation in the mouse oocyte cumulus cell complex. Biol. Reprod. 45, 851–9.CrossRefGoogle ScholarPubMed
Fuku, E., Xia, L. & Downey, B.R. (1995). Ultrastructural changes in bovine oocytes cryopreserved by vitrification. Cryobiology 32, 139–56.CrossRefGoogle ScholarPubMed
Gilchrist, R.B., Ritter, L.J. & Armstrong, D.T. (2004). Oocyte–somatic cell interactions during follicle development in mammals. Anim. Reprod. Sci. 82, 431–46.CrossRefGoogle ScholarPubMed
Goud, A.P., Goud, P.T., Qian, C., Van Der, E.J., Maele, G.V. & Dhont, M. (2000). Cryopreservation of human germinal vesicle stage and in vitro matured MII oocytes: influence of cryopreservation media on the survival, fertilisation, and early cleavage divisions. Fertil. Steril. 74, 487–94.CrossRefGoogle Scholar
Hochi, S., Ito, K., Hirabayashi, M., Ueda, M., Kimura, K. & Hanada, A. (1997). Effect of nuclear stages during in vitro maturation on the survival of bovine oocytes following vitrification. Theriogenology 47, 345.CrossRefGoogle Scholar
Hong, S.W., Chung, H.M., Lim, J.M., Ko, J.J., Yoon, T.K., Yee, B. & Cha, K.Y. (1999). Improved human oocytes development after vitrification: a comparision of thawing methods. Fertil. Steril. 72, 142–6.CrossRefGoogle Scholar
Huang, W.T. & Holtz, W. (2002). Effects of meiotic stages, cryoprotectants, cooling and vitrification on the cryopreservation of porcine oocytes. Asian–Australasian Journal of Animal Sciences. Asian–Australasian Association of Animal Production Societies, Kyunggi-do, Korea Republic 15 (4), 485–93.Google Scholar
Katayama, K.P., Stehlik, J., Kuwayama, M., Kato, O. & Stehlik, E. (2003). High survival rate of vitrified human oocytes results in clinical pregnancies. Fertil. Steril. 80, 223–4.CrossRefGoogle Scholar
Liu, R.H., Sun, Q.Y., Li, Y.H., Jiao, L.H. & Wang, W.H. (2003). Maturation of porcine oocytes after cooling at the germinal vesicle stage. Zygote 11, 299–305.CrossRefGoogle ScholarPubMed
Luna, H.S., Ferrari, I. & Rumpf, R. (2001). Influence of stage of maturation of bovine oocytes at time of vitrification on the incidence of diploid metaphase II at completion of maturation. Anim. Reprod. Sci. 68, 23–8.CrossRefGoogle ScholarPubMed
Men, H., Monson, R.L. & Rutledge, J.J. (2002). Effect of meiotic stages and maturation protocols on bovine oocyte's resistance to cryopreservation. Theriogenology 57, 1095–103.CrossRefGoogle ScholarPubMed
Men, H., Monson, R.L., Parrish, J.J. & Rutledge, J.J. (2003). Detection of DNA damage in bovine metaphase II oocytes resulting from cryopreservation. Mol. Reprod. Dev. 64, 245–50.CrossRefGoogle ScholarPubMed
Mori, T., Amano, T. & Shimizu, H. (2000). Roles of gap junctional communication of cumulus cells in cytoplasmic maturation of porcine oocytes cultured in vitro. Biol. Reprod. 62, 913–9.CrossRefGoogle ScholarPubMed
Paynter, S.J. & Fuller, B.J. (2004). Cryopreservation of the female reproductive cells: current concepts and controversies. Biol. Reprod. 1, 1–27.Google Scholar
Quinn, P., Kerin, J.F. & Warnes, G.M. (1985). Improved pregnancy rate in human in vitro fertilization with the use of a medium based on the composition of human tubal fluid. Fertil. Steril. 44, 493–8.CrossRefGoogle Scholar
Rodriguez, K.F. & Farin, C.E. (2004). Gene transcription and regulation of oocyte maturation. Reprod. Fertil. Dev. 16, 55–67.CrossRefGoogle ScholarPubMed
Ruppert-Lingham, C.J., Paynter, S.J., Godfrey, J., Fuller, B.J. & Shaw, R.W. (2003). Developmental potential of murine germinal vesicle stage cumulus–oocyte complexes following exposure to dimethylsulphoxide or cryopreservation: loss of membrane integrity of cumulus cells after thawing. Hum. Reprod. 18, 392–8.CrossRefGoogle ScholarPubMed
Ruppert-Lingham, C.J., Paynter, S.J., Godfrey, J., Fuller, B.J. & Shaw, R.W. (2006). Membrane integrity and development of immature murine cumulus–oocyte complexes following slow cooling to –60°C: the effect of immediate rewarming, plunging into LN2 and two-controlled-rate-stage cooling. Cryobiology 52 (2), 219–27.CrossRefGoogle Scholar
Sharma, G.T. & Loganathasamy, K. (2007). Effect of meiotic stages during in vitro maturation on the survival of vitrified-warmed buffalo oocytes. Vet. Res. Commun. 31, 881–93.CrossRefGoogle ScholarPubMed
Stephen, M. D. (2001). A gap-junction-mediated signal rather than an external paracrine factor predominates during meiotic induction in isolated mouse oocytes. Zygote 9, 71–82.Google Scholar
Vajta, G., Holm, P., Kuwayama, M., Booth, P.J., Jacobsen, H., Greve, T. & Callesen, H. (1998). Open pulled straw (OPS) vitrification: a new way to reduce cryoinjuries of bovine ova and embryos. Mol. Reprod. Dev. 51, 53–8.3.0.CO;2-V>CrossRefGoogle ScholarPubMed