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Role of Mael in early oogenesis and during germ-cell differentiation from embryonic stem cells in mice in vitro

Published online by Cambridge University Press:  15 February 2013

I. Bahena ,
E. Xu ,
M. Betancourt ,
E. Casas ,
Y. Ducolomb ,
C. González  and
E. Bonilla
I. Bahena
Affiliation:
Department of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, México DF 09340, México.
E. Xu
Affiliation:
Feinberg School of Medicine, Northwestern University, Chicago IL 60611, USA.
M. Betancourt
Affiliation:
Department of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, México DF 09340, México.
E. Casas
Affiliation:
Department of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, México DF 09340, México.
Y. Ducolomb
Affiliation:
Department of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, México DF 09340, México.
C. González
Affiliation:
Department of Health Sciences, Universidad Autónoma Metropolitana-Iztapalapa, México DF 09340, México.
E. Bonilla*
Affiliation:
Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco # 186, Col Vicentina, México DF 09340, México. Feinberg School of Medicine, Northwestern University, Chicago IL 60611, USA.
*
All correspondence to: Edmundo Bonilla. Departamento de Ciencias de la Salud, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco # 186, Col Vicentina, México DF 09340, México. Tel: +52 55 58046557. Fax: +52 55 58044727. e-mail: mundo@xanum.uam.mx
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Summary

In a previous study, we have identified a set of conserved spermatogenic genes whose expression is restricted to testis and ovary and that are developmentally regulated. One of these genes, the transcription factor Mael, has been reported to play an essential role in mouse spermatogenesis. Nevertheless, the role of Mael in mouse oogenesis has not been defined. In order to analyse the role of Mael in mouse oogenesis, the expression of this gene was blocked during early oogenesis in mouse in vitro using RNAi technology. In addition, the role of Mael during differentiation of embryonic stem cells (ESC) into germ cells in vitro was analysed. Results show that downregulation of Mael by a specific short interfering RNA disrupted fetal oocyte growth and differentiation in fetal ovary explants in culture and the expression of several germ-cell markers in ESC during their differentiation. These results suggest that there is an important role for Mael in early oogenesis and during germ-cell differentiation from embryonic stem cells in mouse in vitro.

Keywords

ESCGerm cellsMaelOogenesisRNAisiRNA
Type
Research Article
Information
Zygote , Volume 22 , Issue 4 , November 2014 , pp. 513 - 520
Copyright
Copyright © Cambridge University Press 2013 

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