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Sperm penetration in parthenogenetic mouse embryos triggers a plasma membrane block to polyspermy

Published online by Cambridge University Press:  26 September 2008

Marek Maleszewski  and
Anna Bielak
Marek Maleszewski*
Affiliation:
Institute of Zoology, University of Warsaw, Warsaw, Poland
Anna Bielak
Affiliation:
Institute of Zoology, University of Warsaw, Warsaw, Poland
*
Marek Maleszewski, Department of Embryology, Institute of Zoology, University of Warsaw, 00-927 Warsaw 64, Poland. Fax: (48 22) 26 86 24.
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Summary

Mouse oocytes activated parthenogenetically do not generate a plasma membrane block against spermatozoa over the first three cell cycles. We show that they lose this fusibility spontaneously at the 8-cell stage. Insemination of 1-cell parthenogenetic embryos induces loss of fusibility earlier, at the 2-cell stage. This observation suggests that incorporation of the sperm cell membrane components into the oolemma may be responsible for the development of the membrane block.

Keywords

Block to polyspermyIn vitro fertilisationEgg plasma membraneParthenogenetic embryoSperm fusion
Type
Review Article
Information
Zygote , Volume 1 , Issue 3 , August 1993 , pp. 237 - 242
Copyright
Copyright © Cambridge University Press 1993

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