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Survival of Macrobrachium amazonicum embryos submitted to cooling

Published online by Cambridge University Press:  12 April 2017

Arthur Vinícius Lourenço Ferreira ,
Moisés Fernandes Martins ,
Míriam Luzia Nogueira Martins de Sousa ,
Aldeney Andrade Soares Filho  and
Célia Maria de Souza Sampaio
Arthur Vinícius Lourenço Ferreira*
Affiliation:
Department of Biology, State University of Ceará, Fortaleza, CE, 60740–000, Brazil.
Moisés Fernandes Martins
Affiliation:
Postgraduate Program in Veterinary Science, State University of Ceará—UECE, Fortaleza, CE, Brazil.
Míriam Luzia Nogueira Martins de Sousa
Affiliation:
Postgraduate Program in Veterinary Science, State University of Ceará—UECE, Fortaleza, CE, Brazil.
Aldeney Andrade Soares Filho
Affiliation:
Department of Fishing Engineering, Federal University of Ceará, Fortaleza, CE, Brazil.
Célia Maria de Souza Sampaio
Affiliation:
Department of Biology, State University of Ceará, Fortaleza, CE, Brazil.
*
All correspondence to: Arthur Vinícius Lourenço Ferreira. Department of Biology, State University of Ceará, Fortaleza, CE, 60740–000, Brazil. Tel:/Fax: +85 3101 9927. E-mail: arthurvinicius.lf@gmail.com
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Summary

Cooling techniques have several applications for reproduction in aquaculture. However, few studies have sought to create protocols for cooling and cryopreservation of Macrobrachium amazonicum embryos. Thus, the objective of this work was to verify the survival of M. amazonicum embryos and the correlation between embryonic volume and mortality of M. amazonicum embryos after cooling. Embryo pools were collected from three females and divided into two treatment groups: dimethyl sulfoxide (DMSO) 3% and ethylene glycol (EG) 0.5%, both of them associated with 2 M sucrose. Positive and negative control groups consisted of seawater 10%. Aliquots of 10 µg of embryos were placed in Falcon® tubes containing a cryoprotectant solution and submitted directly to the test temperature of 2°C for 2 and 6 h of cooling. Further analysis of survival and embryonic volume were performed under a stereoscopic microscope. Data were subjected to analysis of variance (ANOVA), and means were compared using the Tukey test at 5%. The highest embryonic survival rate was observed after the shortest storage time for both the DMSO 3% and the 0.5% EG groups, with survival rates of 84.8 ± 3.9 and 79.7 ± 2.8%, respectively. There was a reduction in survival after 24 h, with the DMSO 3% group presenting a survival rate of 71.7 ± 6.6%, and the EG 0.5% group, 66 ± 6.9%. Survival showed a statistically significant difference when compared with the positive controls after 2 h and 24 h of cooling, with 99 ± 0.5% and 95.8 ± 1.5% survival rates, respectively. There was no significant statistical difference in the embryonic volume, but it was possible to observe a change in the appearance of the embryos, from a translucent coloration to an opaque white or brownish coloration, after 24 h in incubators. Thus, it can be concluded that survival is inversely proportional to storage time and that, although there was no change in the embryonic volume after cooling, a change in the appearance of embryos could be observed.

Keywords

Amazonian prawnCryopreservationCryoprotectantsProtocol
Type
Research Article
Information
Zygote , Volume 25 , Issue 3 , June 2017 , pp. 288 - 295
Copyright
Copyright © Cambridge University Press 2017 

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References

Alfaro, J., Komen, J. & Huisman, E.A. (2001). Cooling, cryoprotectant and hypersaline sensitivity of penaeid shrimp embryos and nauplius larvae. Aquaculture 195, 353–66.CrossRefGoogle Scholar
Bambozzi, A.F., Mattos, L.A., Mello, M.R.B. & Oshiro, L.M.Y. (2014). Criopreservação do espermatóforo e da massa espermática do camarão branco litopenaeus schmitti post mortem. B. Inst. Pesca 40, 49–60.Google Scholar
Castro, S.V., Carvalho, A.D.A., Silva, C.G., Faustino, L.R., Figueiredo, J.R. & Rodrigues, A.R. (2011). Agentes crioprotetores intracelulares: características e utilização na criopreservação de tecido ovariano e oócitos. Acta Sci. Vet. 39, 957.Google Scholar
Chapelle, S. (1986). Aspects of phospholipid metabolism in crustaceans as related to changes in environmental temperatures and salinities. Comp. Biochem. Physiol. 84B, 423–39.Google Scholar
Diwan, A.D., Ayyappan, S., Lal, K.K. & Lakra, W.S. (2010). Cryopreservation of fish gametes and embryos. Indian J. Anim. Sci. 80, 109–24.Google Scholar
Ferreira, A.V.L., Castro, E.J.T., Barbosa, M.A.S., Sousa, M.L.N.S., Paiva, M.A.N., Soares Filho, A.A. & Sampaio, C.M.S. (2015). Toxicity of cryoprotectants agents in freshwater prawn embryos of Macrobrachium amazonicum . Zygote 26, 813–20.CrossRefGoogle Scholar
Fornari, D. C., Streit, D.P. Jr, Tonissi, L.B., Povh, J.A., Sirol, R.N. & Ribeiro, P.R. (2014). Crioprotetores para o resfriamento de embriões de Piracanjuba Brycon orbignyanus (Valenciennes, 1850). Semina 35, 2809–16.Google Scholar
Harrison, K.E. (1990). The role of nutrition in maturation, reproduction and embryonic development of decapod crustaceans: a review. J. Shellfish Res. 9, 1–28.Google Scholar
Hays, L.M., Crowe, J.H., Wolkers, W. & Rudenko, S. (2001). Factors affecting leakage of trapped solutes from phospholipid vesicles during thermotropic phase transitions. Cryobiology 42, 88–102.CrossRefGoogle ScholarPubMed
Huang, X., Zhuang, P., Zhang, L., Feng, G., Liu, J., Zhang, T. & Zhao, F. (2013). Effects of different vitrificant solutions on the embryos of the Chinese Mitten Crab Eriocheir sinensis (Decapoda, Brachyura). Crustaceana 86, 1–15.CrossRefGoogle Scholar
Lin, C., Chiao-Chuan, H. & Tsai, S. (2013). Effect of thermal injury on embryos of banded coral shrimp (Stenopus hispidus) under hypothermal conditions. Cryobiology 66, 3–7.CrossRefGoogle ScholarPubMed
Liu, B. & Li, X. (2008). Preliminary studies on cryopreservation of Sydney Rock Oyster (Saccostrea glomerata) larvae. J. Shellfish Res. 27, 1125–8.CrossRefGoogle Scholar
Maciel, C.R. & Valenti, W.C. (2009). Biology, fisheries, and aquaculture of the amazon river prawn Macrobrachium amazonicum: a review. Nauplius 17, 61–79.Google Scholar
Mazur, P., Schneider, U. & Mahowald, A.P. (1992). Characteristics and kinetics of subzero chilling injury in Drosophila embryos. Cryobiology 29, 39–68.CrossRefGoogle ScholarPubMed
Miliorini, A.B. (2012). Resfriamento e congelamento de embriões de dourado (Salminus brasiliensis), piracanjuba (Brycon orbignyanus) e piapara (Leporinus obtusidens). 2012. 138f. Tese (Doutorado em Ciências Veterinárias)—Universidade Federal de Lavras, Lavras, Minas Gerais.Google Scholar
Odinetz-Collart, O. & Rabelo, H. (1996). Variation in egg size of the fresh-water prawn Macrobrachium amazonicum (Decapoda: Palaemonidae). J. Crust. Biol. 16, 684–8.CrossRefGoogle Scholar
Ravid, T., Tietz, A., Khayat, M., Boehm, E., Michelis, R. & Lubzens, E. (1999). Lipid accumulation in the ovaries of a marine shrimp Penaeus semisulcatus (De Haan). J. Exp. Biol. 202, 1819–29.CrossRefGoogle ScholarPubMed
Seymour, J. (1994). Freezing time at the zoo. New Scientist 1, 21–3.Google Scholar
Subramoniam, T. & Arun, R. (1999). Cryopreservation and aquaculture: a case study with penaeid shrimp larvae. Curr. Sci. 76, 361–8.Google Scholar
Suquet, M., Labbe, C., Puyo, S., Mingant, C., Quittet, B., Boulais, M., Queau, I., Ratiskol, D., Diss, B. & Haffray, P. (2014). Survival, growth and reproduction of larvae from a marine invertebrate, the Pacific Oyster (Crassostrea gigas).PLoS ONE 9, e93486.CrossRefGoogle ScholarPubMed
Tsai, S, Lin, C. (2012). Advantages and applications of cryopreservation in fisheries science. Braz. Arch. Boil. Technol. 55, 425–34.CrossRefGoogle Scholar
Wada, H., Gombos, Z. & Murata, N. (1990). Enhancement of chilling tolerance of a cyanobacterium by genetic manipulation of fatty acid desaturation. Nature 347, 200–3.CrossRefGoogle ScholarPubMed
Wang, H., Li, X., Wang, M., Clarke, S., Gluis, M. & Zhang, Z. (2011). Effects of larval cryopreservation on subsequente development of the blue mussels, Mytilus galloprovincialis Lamarck. Aquac. Res. 42, 1816–23.CrossRefGoogle Scholar
Zhang, T. & Rawson, D.M. (1996). Feasability studies on vitrification of intact Zebrafish (Brachydanio rerio) embryos. Cryobiology 33, 1–13.CrossRefGoogle Scholar